Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Levinson, S.L.; Jacobs, L.H.; Krulwich, T.A.; Li, H.C.
    Purification and characterization of a polyphosphate kinase from Arthrobacter atrocyaneus (1975), J. Gen. Microbiol., 88, 65-74.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.4.1 basic protein activation Sinomonas atrocyanea
2.7.4.1 basic protein can substitute for histone Sinomonas atrocyanea
2.7.4.1 bovine serum albumin activation, can substitute for histone only in the absence of phosphate Sinomonas atrocyanea
2.7.4.1 casein activation, can substitute for histone only in the absence of phosphate Sinomonas atrocyanea
2.7.4.1 histone required for activity Sinomonas atrocyanea
2.7.4.1 phosphate
-
 Sinomonas atrocyanea

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.1 ADP 4 mM, product inhibition Sinomonas atrocyanea
2.7.4.1 ATP free form, above 0.008 mM Sinomonas atrocyanea
2.7.4.1 diphosphate 1 mM, complete inhibition Sinomonas atrocyanea
2.7.4.1 F- 20 mM, complete inhibition Sinomonas atrocyanea
2.7.4.1 Mg2+ free form, above 6 mM, activating below Sinomonas atrocyanea
2.7.4.1 Mn2+ free form, above 2 mM, activating below Sinomonas atrocyanea
2.7.4.1 MnATP2- at high concentrations, above 0.6 mM, activating below Sinomonas atrocyanea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.1 0.53
-
 ATP pH 7.0, 30°C Sinomonas atrocyanea
2.7.4.1 0.53
-
 ATP ATP in form of MnATP2- Sinomonas atrocyanea
2.7.4.1 1.67
-
 phosphate pH 7.0, 30°C Sinomonas atrocyanea

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.1 Mg2+ required for activity Sinomonas atrocyanea
2.7.4.1 Mg2+ inhibitory above 6 mM Sinomonas atrocyanea
2.7.4.1 Mg2+ active substrate: MgATP Sinomonas atrocyanea
2.7.4.1 Mn2+ activation, can replace Mg2+ Sinomonas atrocyanea
2.7.4.1 Mn2+ maximal activity at 1.0-2.0 mM, inhibition above 2 mM Sinomonas atrocyanea
2.7.4.1 Mn2+ active substrate: MnATP Sinomonas atrocyanea

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.1 Sinomonas atrocyanea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.1 ammonium sulfate, DEAE-cellulose, Sephadex G-200 Sinomonas atrocyanea

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.1 0.82
-
-
 Sinomonas atrocyanea

Storage Stability

EC Number Storage Stability Organism
2.7.4.1 5°C, at least 6 months Sinomonas atrocyanea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.1 ATP + (phosphate)n incorporates gamma-phosphate of ATP into long-chain polyphosphate molecules Sinomonas atrocyanea ADP + (phosphate)n+1
-
 ?
2.7.4.1 ATP + phosphate
-
 Sinomonas atrocyanea ADP + diphosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.4.1 30
-
 assay at Sinomonas atrocyanea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.1 6 7
-
 Sinomonas atrocyanea

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.4.1 5 8 approx. half-maximal activity at pH 5.0 and pH 8.0 Sinomonas atrocyanea