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Literature summary extracted from

  • Amick, G.D.; Reddy, S.A.G.; Damuni, Z.
    Purification and properties of a protamine kinase from bovine kidney microsomes (1992), Arch. Biochem. Biophys., 297, 80-85.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.11.1 Triton X-100 stabilizes during freeze-thawing procedures Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.1 Ca2+ not Bos taurus
2.7.11.1 Catalytic subunit of protein phosphatase 2A
-
 Bos taurus
2.7.11.1 KCl not Bos taurus
2.7.11.1 additional information EGTA, calmodulin; heparin, Ca2+/calmodulin, phosphatidylserine and/or diolein (in the absence or presence of Ca2+) Bos taurus
2.7.11.1 NaCl not Bos taurus
2.7.11.1 Protein phosphatase 2A 2A1 and 2; ATP; okadaic acid, microcystin-LR; phosphate or NaF prevents; specific protamine kinase inhibitors Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.1 cytosol
-
 Bos taurus 5829
-
2.7.11.1 microsome microsomal kinase is a form of the cytosolic kinase Bos taurus
-
-
2.7.11.1 soluble
-
 Bos taurus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.1 Mg2+
-
 Bos taurus
2.7.11.1 Mn2+ can replace Mg2+ with 20% efficiency Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.1 45000
-
 gel filtration Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.1 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.1 microsomal Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.1 kidney
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.1 7.53
-
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
2.7.11.1 -70°C, in 0.025 M Tris-HCl, pH 7.3, 10% glycerol, 1 mM EDTA, 1 mM benzamidine, 0.1 mM PMSF, 14 mM 2-mercaptoethanol, 0.1% Triton X-100, 12 months Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.1 ATP + casein phosphorylated at 5% the rate of protamine, cytosolic kinase Bos taurus ?
-
 ?
2.7.11.1 ATP + histone H1 not microsomal kinases Bos taurus ?
-
 ?
2.7.11.1 ATP + histone IIB phosphorylated at 8% the rate of protamine, cytosolic kinase Bos taurus ?
-
 ?
2.7.11.1 ATP + protamine no substrates are GTP Bos taurus ADP + O-phosphoprotamine
-
 ?
2.7.11.1 ATP + several 40S ribosomal polypeptides e.g. S6, 2.5 mol phosphate per mol S6 Bos taurus ?
-
 ?
2.7.11.1 ATP + synthetic peptide Arg-Arg-Leu-Ser-Ser-Leu-Arg-Ala Bos taurus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.1 monomer 1 * 45000, SDS-PAGE Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.1 30
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.1 6 9
-
 Bos taurus