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Literature summary extracted from

  • Damuni, Z.; Amick, G.D.; Sneed, T.R.
    Purification and properties of a distinct protamine kinase from the cytosol of bovine kidney cortex (1989), J. Biol. Chem., 264, 6412-6416.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.1 3',5'-AMP not Bos taurus
2.7.11.1 3',5'-AMP i.e. cAMP, activation Bos taurus
2.7.11.1 additional information no activation by cGMP Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.1 Ca2+ mitochondrial kinase, not cytosolic Bos taurus
2.7.11.1 KCl not Bos taurus
2.7.11.1 additional information cGMP, heat-stable inhibitor of cAMP-dependent protein kinase; EGTA, calmodulin; heparin, Ca2+/calmodulin, phosphatidylserine and/or diolein (in the absence or presence of Ca2+); no inhibition by cAMP Bos taurus
2.7.11.1 NaCl not Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.1 cytosol two mitochondrial kinases I and II and a cytosolic kinase Bos taurus 5829
-
2.7.11.1 mitochondrion two mitochondrial kinases I and II and a cytosolic kinase Bos taurus 5739
-
2.7.11.1 soluble
-
 Bos taurus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.1 Mg2+ requirement Bos taurus
2.7.11.1 Mg2+ Km-values: 1.8 mM (cytosolic kinase) and 0.04 mM (mitochondrial kinase) Bos taurus
2.7.11.1 Mg2+ 1.5 mM (mitochondrial kinase) Bos taurus
2.7.11.1 Mg2+ 10 mM (cytosolic kinase) Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.1 43000
-
 gel filtration Bos taurus
2.7.11.1 45000
-
 1 * 45000, SDS-PAGE Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.1 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.1 cytosolic Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.1 kidney cortex Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.1 17.38
-
-
 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.1 ATP + bovine serum albumin mitochondrial protamine kinases Bos taurus ?
-
 ?
2.7.11.1 ATP + bovine serum albumin not cytosolic kinase Bos taurus ?
-
 ?
2.7.11.1 ATP + bovine serum albumin phosphorylated at 48% the rate of protamine Bos taurus ?
-
 ?
2.7.11.1 ATP + casein not mitochondrial protamine kinases Bos taurus ?
-
 ?
2.7.11.1 ATP + casein phosphorylated at 5% the rate of protamine, cytosolic kinase Bos taurus ?
-
 ?
2.7.11.1 ATP + glycogen synthase from rabbit skeletal muscle Bos taurus ?
-
 ?
2.7.11.1 ATP + glycogen synthase mitochondrial protamine kinases Bos taurus ?
-
 ?
2.7.11.1 ATP + glycogen synthase not cytosolic kinase Bos taurus ?
-
 ?
2.7.11.1 ATP + glycogen synthase phosphorylated at 15% the rate of protamine Bos taurus ?
-
 ?
2.7.11.1 ATP + histone H1 mitochondrial protamine kinases Bos taurus ?
-
 ?
2.7.11.1 ATP + histone H1 not cytosolic Bos taurus ?
-
 ?
2.7.11.1 ATP + histone IIB phosphorylated at 8% the rate of protamine, cytosolic kinase Bos taurus ?
-
 ?
2.7.11.1 ATP + histone IIB not mitochondrial protamine kinases Bos taurus ?
-
 ?
2.7.11.1 ATP + protamine preferred substrate Bos taurus ADP + O-phosphoprotamine
-
 ?
2.7.11.1 ATP + protamine no substrates are histone H4, ovalbumin, synthetic peptide poly(Glu,Tyr) (4:1) Bos taurus ADP + O-phosphoprotamine
-
 ?
2.7.11.1 ATP + protamine no substrates are GTP Bos taurus ADP + O-phosphoprotamine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.1 monomer 1 * 45000, SDS-PAGE Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.1 30
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.1 5.7 9 broad, protamine as substrate Bos taurus