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Literature summary extracted from

  • Damuni, Z.; Reed, L.J.
    Purification and properties of a protamine kinase and a type II casein kinase from bovine kidney mitochondria (1988), Arch. Biochem. Biophys., 262, 574-584.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.11.1 diluted solutions are unstable Bos taurus
2.7.11.1 repeated freeze-thawing inactivates Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.1 Ca2+
-
 Bos taurus
2.7.11.1 additional information heparin, Ca2+/calmodulin, phosphatidylserine and/or diolein (in the absence or presence of Ca2+); protein inhibitor of cAMP-dependent protein kinase (protamine as substrate), monoclonal antibodies to catalytic domain of protein kinase C from rat brain Bos taurus
2.7.11.1 NaCl bovine serum albumin, not protamine as substrate Bos taurus
2.7.11.1 O-phosphoprotamine bovine serum albumin, not protamine as substrate Bos taurus
2.7.11.1 spermine bovine serum albumin, not protamine as substrate Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.1 mitochondrion
-
 Bos taurus 5739
-
2.7.11.1 soluble
-
 Bos taurus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.1 Mg2+ 1.5 mM (mitochondrial kinase) Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.1 45000
-
 gel filtration Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.1 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.1 mitochondrial kinase Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.1 kidney
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.1 5.11
-
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
2.7.11.1 -20°C, in 0.05 M imidazole chloride, pH 7.3, 10% glycerol, 1 mM DTT, protease inhibitors, 1 mM EDTA, at least 6 months Bos taurus
2.7.11.1 0-4°C, in 0.05 M imidazole chloride, pH 7.3, 10% glycerol, 1 mM DTT, protease inhibitors, 1 mM EDTA, up to 3 weeks Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.1 ATP + bovine serum albumin phosphorylated at 40-70% the rate of protamine Bos taurus ?
-
 ?
2.7.11.1 ATP + glycogen synthase phosphorylated at 15% the rate of protamine Bos taurus ?
-
 ?
2.7.11.1 ATP + histone H1 phosphorylated at 80% the rate of protamine Bos taurus ?
-
 ?
2.7.11.1 ATP + protamine preferred substrate Bos taurus ADP + O-phosphoprotamine
-
 ?
2.7.11.1 ATP + protamine phosphorylates little, if any activity with branched-chain alpha-keto acid dehydrogenase, pyruvate dehydrogenase, casein, ovalbumin or histone H2B Bos taurus ADP + O-phosphoprotamine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.1 monomer 1 * 45000, SDS-PAGE Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.1 30
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.1 6.1 8.2 protamine as substrate Bos taurus
2.7.11.1 6.7
-
 bovine serum albumin as substrate Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.11.1 5.8 8.8 about 85% of maximal activity at pH 5.8 and about 80% of maximal activity at pH 8.8, protamine as substrate Bos taurus
2.7.11.1 6.6 6.8 about half-maximal activity at pH 6.6 and pH 6.8, bovine serum albumin as substrate Bos taurus