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Literature summary extracted from

  • Ahmad, S.; Scopes, R.K.
    Isolation and properties of a constitutive D-xylulokinase from a novel thermophilic Saccharococcus caldoxylosilyticus DSM 12041 (ATCC 700356) (2002), Enzyme Microb. Technol., 30, 627-632.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.17 0.09
-
 xylulose pH 8.0, 25°C Parageobacillus caldoxylosilyticus
2.7.1.17 0.16
-
 ATP pH 8.0, 25°C Parageobacillus caldoxylosilyticus
2.7.1.17 0.16
-
 ATP ATP in form of MgATP2- Parageobacillus caldoxylosilyticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.17 54000
-
 2 * 54000, SDS-PAGE Parageobacillus caldoxylosilyticus
2.7.1.17 110000 115000 gel filtration Parageobacillus caldoxylosilyticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.17 additional information Parageobacillus caldoxylosilyticus enzyme is constitutively expressed ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.17 Parageobacillus caldoxylosilyticus
-
 DSM 12041
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.17
-
 Parageobacillus caldoxylosilyticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.17 60
-
 at 25°C Parageobacillus caldoxylosilyticus
2.7.1.17 185
-
 at 65-75°C Parageobacillus caldoxylosilyticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.17 ATP + D-xylulose
-
 Parageobacillus caldoxylosilyticus ADP + D-xylulose 5-phosphate
-
 r
2.7.1.17 additional information enzyme is constitutively expressed Parageobacillus caldoxylosilyticus ?
-
 ?
2.7.1.17 UTP + D-xylulose 15% of the activity with ATP Parageobacillus caldoxylosilyticus UDP + D-xylulose 5-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.17 dimer 2 * 54000, SDS-PAGE Parageobacillus caldoxylosilyticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.17 65 75
-
 Parageobacillus caldoxylosilyticus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.1.17 30 77 30°C: about 50% of maximal activity, 77°C: about 65% of maximal activity Parageobacillus caldoxylosilyticus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.17 65
-
 30 min, Tris buffer, stable up to Parageobacillus caldoxylosilyticus
2.7.1.17 70
-
 30 min, 50% loss of activity Parageobacillus caldoxylosilyticus
2.7.1.17 75
-
 8 mM xylulose, 55% loss of activity after 30 min, 15% loss of activity after 5 min Parageobacillus caldoxylosilyticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.17 8
-
-
 Parageobacillus caldoxylosilyticus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.17 6.7 9 pH 6.7: about 50% of maximal activity, pH 9.0: about 75% of maximal activity Parageobacillus caldoxylosilyticus