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Literature summary extracted from

  • Chen, C.Y.; Dion, S.B.; Kim, C.M.; Benovic, J.L.
    Beta-adrenergic receptor kinase. Agonist-dependent receptor binding promotes kinase activation (1993), J. Biol. Chem., 268, 7825-7831.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.15 beta2-adrenergic receptor interaction of enzyme with human agonist-occupied receptor specifically and significantly enhances peptide phosphorylation, at lower concentrations, enhances both the affinity and catalytic efficiency for peptide phosphorylation Bos taurus
2.7.11.15 rhodopsin interaction of enzyme with light-activated rhodopsin or truncated rhodopsin lacking its C-terminal phosphorylation sites activates peptide phosphorylation, at lower concentrations, enhances both the affinity and catalytic efficiency for peptide phosphorylation, but intact light-activated rhodopsin slightly inhibits the phosphorylation of RRREEEEESAAA Bos taurus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.15 beta-ARK 1 is cloned and expressed in SF9 cells using the baculovirus expression system Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.15 rhodopsin intact light-activated rhodopsin slightly inhibits phosphorylation of RRREEEEESAAA Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.15 additional information
-
 additional information kinetics of peptide phosphorylation, influence of activated rhodopsin and beta2-AR on the Km value for different peptide substrates Bos taurus
2.7.11.15 0.72
-
 RRREEEEESAAA pH 7.4, 30°C, recombinant beta-ARK 1 Bos taurus
2.7.11.15 3.3
-
 RRRASAAASAA pH 7.4, 30°C, recombinant beta-ARK 1 Bos taurus
2.7.11.15 3.4
-
 RRRASpASAA pH 7.4, 30°C, recombinant beta-ARK 1 Bos taurus
2.7.11.15 4.6
-
 RRRASpAAASAA pH 7.4, 30°C, recombinant beta-ARK 1 Bos taurus
2.7.11.15 5.1
-
 RRRAEASAA pH 7.4, 30°C, recombinant beta-ARK 1 Bos taurus
2.7.11.15 5.4
-
 RRRASASAA pH 7.4, 30°C, recombinant beta-ARK 1 Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.15 ATP + beta-adrenergic receptor Bos taurus natural substrate: beta2-adrenergic receptor ADP + phospho-beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + beta-adrenergic receptor Bos taurus agonist-occupied form of the receptor ADP + phospho-beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + beta-adrenergic receptor Bos taurus involved in homologous desensitization of beta-adrenergic receptor ADP + phospho-beta-adrenergic receptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.15 Bos taurus
-
 beta-ARK 1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.15 recombinant beta-ARK 1 expressed in Sf9 cells Bos taurus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.15 1
-
 about, recombinant beta-ARK 1, expressed in SF9 cells Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.15 ATP + beta-adrenergic receptor specifically phosphorylates the agonist-occupied form of the receptor Bos taurus ADP + phospho-beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + beta-adrenergic receptor human beta2-adrenergic receptor Bos taurus ADP + phospho-beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + beta-adrenergic receptor beta-ARK 1, substrate specificity, the overall topological structure of the activated receptor plays a key role in regulating signal-dependent receptor phosphorylation Bos taurus ADP + phospho-beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + beta-adrenergic receptor natural substrate: beta2-adrenergic receptor Bos taurus ADP + phospho-beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + beta-adrenergic receptor agonist-occupied form of the receptor Bos taurus ADP + phospho-beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + beta-adrenergic receptor involved in homologous desensitization of beta-adrenergic receptor Bos taurus ADP + phospho-beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + peptide beta-ARK 1 prefers peptides containing acidic residues on the N-terminal side of a serine or threonine, presence of activated receptor enhances peptide phosphorylation Bos taurus ADP + phosphopeptide
-
 ?
2.7.11.15 ATP + peptide synthetic Bos taurus ADP + phosphopeptide
-
 ?
2.7.11.15 ATP + rhodopsin light-dependent, actual substrate: light-bleached rhodopsin Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.15 ATP + rhodopsin in form of bovine rod outer segments Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.15 ATP + RRRAEAAASAAA
-
 Bos taurus ADP + RRRAEAAApSAAA
-
 ?
2.7.11.15 ATP + RRRAEASAA poor peptide substrate Bos taurus ADP + RRRAEApSAA
-
 ?
2.7.11.15 ATP + RRRASAAASAA poor peptide substrate Bos taurus ?
-
 ?
2.7.11.15 ATP + RRRASASAA poor peptide substrate Bos taurus ?
-
 ?
2.7.11.15 ATP + RRRASpAAASAA poor peptide substrate, higher catalytic efficiency than RRRASAAASAA Bos taurus ADP + RRRASpAAASpAA
-
 ?
2.7.11.15 ATP + RRRASpASAA poor peptide substrate Bos taurus ?
-
 ?
2.7.11.15 ATP + RRREEEEESAAA good peptide substrate, but the activated receptor is a much better substrate Bos taurus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.15 30
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.15 7.4
-
 assay at Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.15 ATP
-
 Bos taurus