BRENDA - Enzyme Database

Activation of rat liver AMP-activated protein kinase by kinase kinase in a purified, reconstituted system. Effects of AMP and AMP analogues

Weekes, J.; Hawley, S.A.; Corton, J.; Shugar, D.; Hardie, D.G.; Eur. J. Biochem. 219, 751-757 (1994)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.11.3
8-Aza-9-deaza-AMP
activation, i.e. formycin A-5'-monophosphate, can replace AMP
Rattus norvegicus
2.7.11.3
8-Aza-9-deaza-IMP
slight activation, i.e. formycin B-5'-monophosphate
Rattus norvegicus
2.7.11.3
additional information
no activation by formycin A or B
Rattus norvegicus
2.7.11.31
Reductase kinase kinase
activation, i.e. EC 2.7.1.110, in the presence of MgATP2-
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.11.3
adenosine(5')tetraphospho(5')adenosine
i.e. AP4A, inhibits in the presence of AMP
Rattus norvegicus
2.7.11.31
adenosine-5'-tetraphospho-5'-adenosine
i.e. AP4A, inhibits in the presence of AMP
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.11.3
cytosol
-
Rattus norvegicus
5829
-
2.7.11.31
cytosol
-
Rattus norvegicus
5829
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.11.3
Mg2+
requirement, actual substrate: MgATP
Rattus norvegicus
2.7.11.31
Mg2+
requirement, actual substrate: MgATP2-
Rattus norvegicus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.11.3
ATP + dephospho-[[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
Rattus norvegicus
phosphorylation activates EC 2.7.1.109
ADP + [[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
-
Rattus norvegicus
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.11.3
Rattus norvegicus
-
-
-
2.7.11.31
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.11.3
partial
Rattus norvegicus
2.7.11.31
partial
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.11.3
liver
-
Rattus norvegicus
-
2.7.11.31
liver
-
Rattus norvegicus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.11.3
ATP + dephospho-[[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
i.e. AMP-activated protein kinase
644972
Rattus norvegicus
ADP + [[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
-
644972
Rattus norvegicus
-
2.7.11.3
ATP + dephospho-[[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
phosphorylates catalytic subunit of EC2.7.1.109
644972
Rattus norvegicus
ADP + [[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
-
644972
Rattus norvegicus
-
2.7.11.3
ATP + dephospho-[[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
phosphorylation activates EC 2.7.1.109
644972
Rattus norvegicus
ADP + [[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
-
644972
Rattus norvegicus
-
2.7.11.31
ATP + [hydroxymethylglutaryl-CoA reductase (NADPH)]
-
644972
Rattus norvegicus
ADP + [hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate
-
-
-
?
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.7.11.3
30
-
-
Rattus norvegicus
2.7.11.31
30
-
assay at
Rattus norvegicus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.7.11.3
AMP
allosteric activator, the allosteric effect and the promotion of phosphorylation and activation by the kinase kinase are due to binding of AMP to a single site on the kinase
Rattus norvegicus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.11.3
8-Aza-9-deaza-AMP
activation, i.e. formycin A-5'-monophosphate, can replace AMP
Rattus norvegicus
2.7.11.3
8-Aza-9-deaza-IMP
slight activation, i.e. formycin B-5'-monophosphate
Rattus norvegicus
2.7.11.3
additional information
no activation by formycin A or B
Rattus norvegicus
2.7.11.31
Reductase kinase kinase
activation, i.e. EC 2.7.1.110, in the presence of MgATP2-
Rattus norvegicus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.7.11.3
AMP
allosteric activator, the allosteric effect and the promotion of phosphorylation and activation by the kinase kinase are due to binding of AMP to a single site on the kinase
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.11.3
adenosine(5')tetraphospho(5')adenosine
i.e. AP4A, inhibits in the presence of AMP
Rattus norvegicus
2.7.11.31
adenosine-5'-tetraphospho-5'-adenosine
i.e. AP4A, inhibits in the presence of AMP
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.11.3
cytosol
-
Rattus norvegicus
5829
-
2.7.11.31
cytosol
-
Rattus norvegicus
5829
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.11.3
Mg2+
requirement, actual substrate: MgATP
Rattus norvegicus
2.7.11.31
Mg2+
requirement, actual substrate: MgATP2-
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.11.3
ATP + dephospho-[[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
Rattus norvegicus
phosphorylation activates EC 2.7.1.109
ADP + [[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
-
Rattus norvegicus
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.11.3
partial
Rattus norvegicus
2.7.11.31
partial
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.11.3
liver
-
Rattus norvegicus
-
2.7.11.31
liver
-
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.11.3
ATP + dephospho-[[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
i.e. AMP-activated protein kinase
644972
Rattus norvegicus
ADP + [[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
-
644972
Rattus norvegicus
-
2.7.11.3
ATP + dephospho-[[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
phosphorylates catalytic subunit of EC2.7.1.109
644972
Rattus norvegicus
ADP + [[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
-
644972
Rattus norvegicus
-
2.7.11.3
ATP + dephospho-[[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
phosphorylation activates EC 2.7.1.109
644972
Rattus norvegicus
ADP + [[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
-
644972
Rattus norvegicus
-
2.7.11.31
ATP + [hydroxymethylglutaryl-CoA reductase (NADPH)]
-
644972
Rattus norvegicus
ADP + [hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate
-
-
-
?
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.7.11.3
30
-
-
Rattus norvegicus
2.7.11.31
30
-
assay at
Rattus norvegicus