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Literature summary extracted from

  • Beg, Z.H.; Stonik, J.A.; Brewer, H.B.
    Phosphorylation and modulation of the enzymic activity of native and protease-cleaved purified hepatic 3-hydroxy-3-methylglutaryl-coenzyme A reductase by a calcium/calmodulin-dependent protein kinase (1987), J. Biol. Chem., 262, 13228-13240.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.11.31 highly labile enzyme Rattus norvegicus
2.7.11.31 protease inhibitors stabilizes enzyme Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.31 EGTA
-
 Rattus norvegicus
2.7.11.31 Inhibitor W-7 specific Ca2+/calmodulin-dependent kinase inhibitor Rattus norvegicus
2.7.11.31 Trifluperazine specific Ca2+/calmodulin-dependent kinase inhibitor Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.31 0.00085
-
 hydroxymethylglutaryl-CoA reductase pH 6.5, 30°C, low-MW kinase Rattus norvegicus
2.7.11.31 0.0029
-
 histone H1 pH 6.5, 30°C, low-MW kinase Rattus norvegicus
2.7.11.31 0.028
-
 ATP pH 6.5, 30°C, low-MW kinase Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.31 cytosol
-
 Rattus norvegicus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.31 Ca2+
-
 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.31 110000 125000 low-MW kinase, gel filtration Rattus norvegicus
2.7.11.31 560000 600000 high-MW kinase, gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.31 ATP + [hydroxymethylglutaryl-CoA reductase (NADPH)] Rattus norvegicus inactivates EC 1.1.1.34 by phosphorylation ADP + [hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.31 Rattus norvegicus
-
 Sprague-Dawley
-
2.7.11.31 Rattus norvegicus Sprague-Dawley
-
 Sprague-Dawley
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.31 low-MW kinase Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.31 brain
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.31 0.292
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.31 ATP + casein relative kinase activity for low-MW kinase 8%, high MW-kinase 48% Rattus norvegicus ADP + casein phosphate
-
 ?
2.7.11.31 ATP + casein relative kinase activity for low-MW kinase 8%, high MW-kinase 48% Rattus norvegicus Sprague-Dawley ADP + casein phosphate
-
 ?
2.7.11.31 ATP + dephospho-alpha,beta-tubulin relative kinase activity high MW-kinase 15% Rattus norvegicus ADP + [alpha,beta-tubulin] phosphate
-
 ?
2.7.11.31 ATP + glycogen synthase relative kinase activity for low-MW kinase 7%, high MW-kinase 87% Rattus norvegicus ADP + [glycogen synthase] phosphate
-
 ?
2.7.11.31 ATP + heavy meromyosin relative kinase activity for low-MW kinase 2%, high MW-kinase 100% Rattus norvegicus ADP + [heavy meromyosin] phosphate
-
 ?
2.7.11.31 ATP + histone H1
-
 Rattus norvegicus ADP + phosphohistone H1
-
 ?
2.7.11.31 ATP + histone H1 (IIIS) histones are better substrates for high-MW kinase than hydroxymethylglutaryl-CoA reductase, relative kinase activity for low-MW kinase 275%, high MW-kinase 103% Rattus norvegicus ADP + [histone H1 (IIIS)] phosphate
-
 ?
2.7.11.31 ATP + histone II-S relative kinase activity for low-MW kinase 38%, high MW-kinase 159% Rattus norvegicus ADP + [histone II-S] phosphate
-
 ?
2.7.11.31 ATP + histone VIIIS relative kinase activity for low-MW kinase 65%, high MW-kinase 141% Rattus norvegicus ADP + [histone VIIIS] phosphate
-
 ?
2.7.11.31 ATP + MAP-2 relative kinase activity for low-MW kinase 14%, high MW-kinase 566% Rattus norvegicus ADP + MAP-2 phosphate
-
 ?
2.7.11.31 ATP + myelin basic protein moderate substrate for low-MW kinase, better than hydroxymethylglutaryl-CoA reductase for high-MW kinase, relative kinase activity for low-MW kinase 36%, high MW-kinase 238% Rattus norvegicus ADP + [myelin basic protein] phosphate
-
 ?
2.7.11.31 ATP + myosin mixed light chains relative kinase activity for low-MW kinase 4%, high MW-kinase 27% Rattus norvegicus ADP + [myosin mixed light chains] phosphate
-
 ?
2.7.11.31 ATP + phosphorylase B relative kinase activity high MW-kinase 12% Rattus norvegicus ADP + [phosphorylase B] phosphate
-
 ?
2.7.11.31 ATP + phosvitin relative kinase activity for low-MW kinase 2%, high MW-kinase 2% Rattus norvegicus ADP + phosvitin phosphate
-
 ?
2.7.11.31 ATP + phosvitin relative kinase activity for low-MW kinase 2%, high MW-kinase 2% Rattus norvegicus Sprague-Dawley ADP + phosvitin phosphate
-
 ?
2.7.11.31 ATP + protamine relative kinase activity for low-MW kinase 24%, high MW-kinase 38% Rattus norvegicus ADP + protamine phosphate
-
 ?
2.7.11.31 ATP + protamine relative kinase activity for low-MW kinase 24%, high MW-kinase 38% Rattus norvegicus Sprague-Dawley ADP + protamine phosphate
-
 ?
2.7.11.31 ATP + synapsin 1 as good substrate as hydroxymethylglutaryl-CoA reductase, relative kinase activity for low-MW kinase 151%, high MW-kinase 103% Rattus norvegicus ADP + [synapsin 1] phosphate
-
 ?
2.7.11.31 ATP + [hydroxymethylglutaryl-CoA reductase (NADPH)]
-
 Rattus norvegicus ADP + [hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate
-
 ?
2.7.11.31 ATP + [hydroxymethylglutaryl-CoA reductase (NADPH)] inactivates EC 1.1.1.34 by phosphorylation Rattus norvegicus ADP + [hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate
-
 ?
2.7.11.31 additional information autophosphorylation in absence of substrate Rattus norvegicus ?
-
 ?
2.7.11.31 additional information incorporates 0.5 mol phosphate/mol MW 53000 enzyme substrate fragment, 2 mol phosphate/mol native enzyme substrate Rattus norvegicus ?
-
 ?
2.7.11.31 additional information autophosphorylation in absence of substrate Rattus norvegicus Sprague-Dawley ?
-
 ?
2.7.11.31 additional information incorporates 0.5 mol phosphate/mol MW 53000 enzyme substrate fragment, 2 mol phosphate/mol native enzyme substrate Rattus norvegicus Sprague-Dawley ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.31 6.5
-
 low-MW kinase Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.11.31 5.5 7 about half-maximal activity at pH 5.5 and 7.0, low-MW kinase Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.31 Calmodulin requirement, Ca2+/calmodulin dependent kinase, no phosphorylation of substrate observed in absence Rattus norvegicus