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Literature summary extracted from

  • Wannet, W.J.; Op den Camp, H.J.; Wisselink, H.W.; van der Drift, C.; Van Griensven, L.J.; Vogels, G.D.
    Purification and characterization of trehalose phosphorylase from the commercial mushroom Agaricus bisporus (1998), Biochim. Biophys. Acta, 1425, 177-188.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.231 AMP stimulates phosphorolytic activity at 10 mM Agaricus bisporus
2.4.1.231 ATP stimulates synthetic activity at 10 mM Agaricus bisporus

General Stability

EC Number General Stability Organism
2.4.1.231 highly unstable during purification, glycerol, phosphate, dithiothreitol and PMSF stabilize Agaricus bisporus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.231 1-deoxynojirimycin 88% inhibition at 10 mM Agaricus bisporus
2.4.1.231 ADP 34% inhibition at 10 mM Agaricus bisporus
2.4.1.231 ATP inhibits trehalose degradation, but stimulates synthetic activity Agaricus bisporus
2.4.1.231 CoCl2 39% inhibition at 1 mM Agaricus bisporus
2.4.1.231 CuSO4 37% inhibition at 1 mM Agaricus bisporus
2.4.1.231 phosphate inhibits synthetic activity Agaricus bisporus
2.4.1.231 validamycin A 99% inhibition at 10 mM Agaricus bisporus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.231 4.7
-
 phosphate 30°C, pH 7 Agaricus bisporus
2.4.1.231 6.3
-
 alpha-D-glucose 1-phosphate 30°C, pH 7 Agaricus bisporus
2.4.1.231 24
-
 alpha-D-glucose 30°C, pH 7 Agaricus bisporus
2.4.1.231 61
-
 alpha,alpha-trehalose 30°C, pH 7 Agaricus bisporus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.231 61000
-
 4 * 61000, SDS-PAGE Agaricus bisporus
2.4.1.231 240000
-
 native PAGE, gel filtration Agaricus bisporus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.231 alpha,alpha-trehalose + phosphate Agaricus bisporus key role in trehalose metabolism alpha-D-glucose + alpha-D-glucose 1-phosphate
-
 r
2.4.1.231 alpha,alpha-trehalose + phosphate Agaricus bisporus Horst U1 key role in trehalose metabolism alpha-D-glucose + alpha-D-glucose 1-phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.231 Agaricus bisporus
-
-
-
2.4.1.231 Agaricus bisporus Horst U1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.231
-
 Agaricus bisporus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.1.231 alpha,alpha-trehalose + phosphate = alpha-D-glucose + alpha-D-glucose 1-phosphate mechanism Agaricus bisporus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.231 fruit body
-
 Agaricus bisporus
-
2.4.1.231 mycelium
-
 Agaricus bisporus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.231 4.35
-
-
 Agaricus bisporus

Storage Stability

EC Number Storage Stability Organism
2.4.1.231 -80°C, stable for at least 6 months Agaricus bisporus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.231 alpha,alpha-trehalose + phosphate
-
 Agaricus bisporus alpha-D-glucose + alpha-D-glucose 1-phosphate
-
 r
2.4.1.231 alpha,alpha-trehalose + phosphate key role in trehalose metabolism Agaricus bisporus alpha-D-glucose + alpha-D-glucose 1-phosphate
-
 r
2.4.1.231 alpha,alpha-trehalose + phosphate
-
 Agaricus bisporus Horst U1 alpha-D-glucose + alpha-D-glucose 1-phosphate
-
 r
2.4.1.231 alpha,alpha-trehalose + phosphate key role in trehalose metabolism Agaricus bisporus Horst U1 alpha-D-glucose + alpha-D-glucose 1-phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.1.231 tetramer 4 * 61000, SDS-PAGE Agaricus bisporus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.231 40
-
 almost complete loss of activity after 60 min Agaricus bisporus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.231 6 7 synthesis of trehalose Agaricus bisporus
2.4.1.231 6 7.5 phosphorolytic reaction Agaricus bisporus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.231 2
-
 phosphate 30°C, pH 7 Agaricus bisporus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.4.1.231 Agaricus bisporus isoelectric focusing between pH 3-9 and pH 3-10 4.8 4.7