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Literature summary extracted from
- Purification and characterization of a trehalose synthase from the basidiomycete Grifola frondosa (1998), Appl. Environ. Microbiol., 64, 4340-4345.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.4.1.231 | Cu2+ | complete inactivation at 1 mM | Grifola frondosa |  |
| 2.4.1.231 | Zn2+ | inactivation at 1 mM | Grifola frondosa | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.231 | 60000 | - |
2 * 60000, SDS-PAGE | Grifola frondosa |
| 2.4.1.231 | 120000 | - |
gel filtration | Grifola frondosa |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.4.1.231 | alpha,alpha-trehalose + phosphate | Grifola frondosa | - |
alpha-D-glucose + alpha-D-glucose 1-phosphate | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.4.1.231 | Grifola frondosa | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.4.1.231 | - |
Grifola frondosa |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.231 | 4.2 | - |
- |
Grifola frondosa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.4.1.231 | alpha,alpha-trehalose + phosphate | - |
Grifola frondosa | alpha-D-glucose + alpha-D-glucose 1-phosphate | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 2.4.1.231 | dimer | 2 * 60000, SDS-PAGE | Grifola frondosa |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.231 | 32.5 | 35 | phosphorolysis | Grifola frondosa |
| 2.4.1.231 | 35 | 37.5 | synthesis of trehalose | Grifola frondosa |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.231 | 35 | - |
stable up to 35°C, decrease of activity above | Grifola frondosa |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.231 | 6.5 | 6.8 | - |
Grifola frondosa |
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