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Literature summary extracted from

  • Guo, L.; Poulton, E.
    Partial purification and characterization of Arabidopsis thaliana UDPG:thiohydroximate glucosyltransferase (1994), Phytochemistry, 36, 1133-1138.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.195 2-mercaptoethanol
-
 Arabidopsis thaliana
2.4.1.195 L-cysteine
-
 Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.195 1,10-phenanthroline causes 50% inhibition Arabidopsis thaliana
2.4.1.195 CuCl2
-
 Arabidopsis thaliana
2.4.1.195 additional information iodoacetic acid and 5,5'-dithio-bis-(2-nitrobenzoic acid) are less inhibitory Arabidopsis thaliana
2.4.1.195 ZnCl2
-
 Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.195 0.27
-
 UDPglucose pH 6.0, 30°C Arabidopsis thaliana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.195 CaCl2 activity is stimulated by thiol reducing agents Arabidopsis thaliana
2.4.1.195 MgCl2 activity is stimulated by thiol reducing agents Arabidopsis thaliana
2.4.1.195 MnCl2 activity is stimulated by thiol reducing agents Arabidopsis thaliana
2.4.1.195 additional information purified enzyme lacks an absolute requirement for metal ions Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.195 57800
-
-
 Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.195 UDPglucose + phenylacetothiohydroximate Arabidopsis thaliana catalyses the penultimate reaction in glucosinolate biosynthesis UDP + desulfoglucotropeolin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.195 Arabidopsis thaliana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.195 partial Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.195 inflorescence
-
 Arabidopsis thaliana
-
2.4.1.195 leaf
-
 Arabidopsis thaliana
-
2.4.1.195 seedling
-
 Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.195 0.561
-
-
 Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.195 UDPglucose + phenylacetothiohydroximate
-
 Arabidopsis thaliana UDP + desulfoglucotropeolin
-
 ?
2.4.1.195 UDPglucose + phenylacetothiohydroximate catalyses the penultimate reaction in glucosinolate biosynthesis Arabidopsis thaliana UDP + desulfoglucotropeolin
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.195 6
-
-
 Arabidopsis thaliana

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.4.1.195 Arabidopsis thaliana
-
-
 4.5