Literature summary extracted from
Tan, X.; Sewell, C.; Yang, Q.; Lindahl, P.A.
Reduction and methyl transfer kinetics of the a subunit from acetyl Coenzyme A synthase (2003), J. Am. Chem. Soc., 125, 318-319.
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
2.3.1.169 |
Fe |
the Ni-Fe4S4-5C cluster of enzyme catalyses the reversible reduction of CO2 to CO and is located in the beta-subunit. |
Moorella thermoacetica |
|
2.3.1.169 |
Fe |
corrinoid/iron-sulfur protein required |
Moorella thermoacetica |
|
2.3.1.169 |
Ni |
the Ni-Fe4S4-5C cluster of enzyme catalyses the reversible reduction of CO2 to CO and is located in the beta-subunit. |
Moorella thermoacetica |
|
2.3.1.169 |
Ni |
enzyme contains nickel in the A-cluster of the enzyme |
Moorella thermoacetica |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.3.1.169 |
Moorella thermoacetica |
- |
- |
- |
Reaction
EC Number |
Reaction |
Comment |
Organism |
Reaction ID |
---|
2.3.1.169 |
acetyl-CoA + a [Co(I) corrinoid Fe-S protein] = CO + CoA + a [methyl-Co(III) corrinoid Fe-S protein] |
pathway |
Moorella thermoacetica |
|
2.3.1.169 |
acetyl-CoA + a [Co(I) corrinoid Fe-S protein] = CO + CoA + a [methyl-Co(III) corrinoid Fe-S protein] |
the Ni-Fe4S4-5C cluster of enzyme catalyses the reversible reduction of CO2 to CO and is located in the beta-subunit. CO generated at this site migrates through the tunnel to the A-cluster, located in the alpha-subunit, where it reacts with CoA and a methyl group to generate acetyl-CoA. During catalysis, the two sites are mechanistically coupled. |
Moorella thermoacetica |
|
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.3.1.169 |
CO + H2O |
the NiFe4S4-5C cluster catalyses the reversible oxidation of CO to CO2 |
Moorella thermoacetica |
CO2 + H+ + electron |
- |
r |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
2.3.1.169 |
tetramer |
alpha,beta, containing two unique Ni-Fe-S active sites connected by a molecular tunnel |
Moorella thermoacetica |