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Literature summary extracted from

  • Wynn, R.M.; Davie, J.R.; Zhi, W.; Cox, R.P.; Chuang, D.T.
    Invitro reconstitution of the 24-meric E2 inner core of bovine mitochondrial branched-chain alpha-keto acid dehydrogenase complex: requirements for chaperonins GroEL and GroES (1994), Biochemistry, 33, 8962-8968.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.168 expression of E2c, residues 161-421, fused to maltose-binding protein, which enhances the yield of the recombinant enzyme, in Escherichia coli Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.168 84000
-
 E2-trimer, sucrose density gradient centrifugation in presence of the chaotropic reagent guanidinium-HCl Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.168 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.168 recombinant E2c, residues 161-421, fused to maltose-binding protein from Escherichia coli Bos taurus

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.3.1.168 E2c, completely unfolded in 4.5 M guanidinium chloride, is diluted 100fold at 25°C and refolded in 5 mM MgATP2- and a 4fold molar excess of chaperonins GroEL and GroES at pH 7.5, full activity is recovered after 45 min, an active GroEL-E2 24-mer is formed Bos taurus
2.3.1.168 in vitro reconstitution of the 24-meric E2 inner core requires the chaperonins GroEL and GroES Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.168 2-methylpropanoyl-CoA + enzyme N6-(dihydrolipoyl)lysine
-
 Bos taurus CoA + enzyme N6-(S-[2-methylpropanoyl]dihydrolipoyl)lysine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.168 More in vitro reconstitution of the 24-meric E2 inner core requires the chaperonins GroEL and GroES Bos taurus
2.3.1.168 More the 24-mer can be separated into active trimers of MW 84 kDa by incubation in 1.5 M guanidinium-HCl at 25°C, process is reversible, and removal of guanidinium-HCl leads to spontenaous reassembly to an active 24-mer Bos taurus

Synonyms

EC Number Synonyms Comment Organism
2.3.1.168 More enzyme is the E2 component of the multienzyme complex branched-chain alpha-keto acid dehydrogenase, i.e. branched-chain 2-oxo acid dehydrogenase Bos taurus