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Literature summary extracted from

  • Chuang, D.T.; Hu, C.C.; Ku, L.S.; Niu, W.L.; Myers, D.E.; Cox, R.P.
    Catalytic and structural properties of the dihydrolipoyl transacylase component of bovine branched-chain alpha-keto acid dehydrogenase (1984), J. Biol. Chem., 259, 9277-9284.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.168 arsenite strong inhibition Bos taurus
2.3.1.168 CoA product inhibition, competitive to acyl-CoA, noncompetitive to dihydrolipoamide Bos taurus
2.3.1.168 Mg2+
-
Bos taurus
2.3.1.168 additional information limited proteolysis by trypsin results in the complete loss of the overall activity of the enzyme complex, but does not affect dihydrolipoyllysine-residue (2-methylpropanoyl)transferase activity; NEM and thiamine diphosphate have no effect Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.168 additional information
-
additional information kinetics Bos taurus
2.3.1.168 0.05
-
isovaleryl-CoA pH 7.4, 37°C Bos taurus
2.3.1.168 0.1
-
isobutyryl-CoA pH 7.4, 37°C Bos taurus
2.3.1.168 0.11
-
acetyl-CoA pH 7.4, 37°C Bos taurus
2.3.1.168 2
-
6,8-bis(sulfanyl)octanamide pH 7.4, 37°C Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.168 52600
-
x * 52600, SDS-PAGE Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.168 2-methylpropanoyl-CoA + enzyme N6-(dihydrolipoyl)lysine Bos taurus
-
CoA + enzyme N6-(S-[2-methylpropanoyl]dihydrolipoyl)lysine
-
r

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.168 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.168
-
Bos taurus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.168 2-methylpropanoyl-CoA + enzyme N6-(dihydrolipoyl)lysine = CoA + enzyme N6-(S-[2-methylpropanoyl]dihydrolipoyl)lysine random bi bi mechanism Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.168 kidney
-
Bos taurus
-
2.3.1.168 liver
-
Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.168 2.38
-
partially purified E2 enzyme Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.168 2-methylpropanoyl-CoA + dihydrolipoamide
-
Bos taurus CoA + S-2-methylpropanoyldihydrolipoamide identification by mass spectrometry r
2.3.1.168 2-methylpropanoyl-CoA + enzyme N6-(dihydrolipoyl)lysine
-
Bos taurus CoA + enzyme N6-(S-[2-methylpropanoyl]dihydrolipoyl)lysine
-
r
2.3.1.168 acetyl-CoA + dihydrolipoamide low activity Bos taurus CoA + S-acetyldihydrolipoamide
-
r
2.3.1.168 isobutyryl-CoA + dihydrolipoamide
-
Bos taurus CoA + S-isobutyryldihydrolipoamide
-
r
2.3.1.168 isovaleryl-CoA + dihydrolipoamide
-
Bos taurus CoA + S-isovaleryldihydrolipoamide
-
r

Subunits

EC Number Subunits Comment Organism
2.3.1.168 ? x * 52600, SDS-PAGE Bos taurus

Synonyms

EC Number Synonyms Comment Organism
2.3.1.168 More enzyme is the E2 component of the multienzyme complex branched-chain alpha-keto acid dehydrogenase, i.e. branched-chain 2-oxo acid dehydrogenase Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.168 37
-
assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.168 7.4
-
assay at Bos taurus