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Literature summary extracted from

  • Saidha, T.; Schiff, J.A.
    Purification and properties of a phenol sulphotransferase from Euglena using L-tyrosine as substrate (1994), Biochem. J., 298, 45-50.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
2.8.2.9 instable to freezing Euglena gracilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.2.1 adenosine 3',5'-bisphosphate competitive Euglena gracilis
2.8.2.1 L-tyrosine O4-sulfate noncompetitive Euglena gracilis
2.8.2.9 adenosine 3',5'-bisphosphate PAP, competitive to 3'-phosphoadenylylsulfate Euglena gracilis
2.8.2.9 Tyrosine O4-sulfate uncompetitive to L-tyrosine Euglena gracilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.1 0.033
-
 L-tyrosine pH 7.0, 37°C Euglena gracilis
2.8.2.1 0.42
-
 3-Nitrophenol pH 7.0, 37°C Euglena gracilis
2.8.2.1 0.5
-
 2-chlorophenol pH 7.0, 37°C Euglena gracilis
2.8.2.1 0.55
-
 4-Chlorophenol pH 7.0, 37°C Euglena gracilis
2.8.2.1 0.714
-
 L-4-hydroxyphenylglycine pH 7.0, 37°C Euglena gracilis
2.8.2.1 1.25
-
 4-nitrophenol pH 7.0, 37°C Euglena gracilis
2.8.2.9 0.033
-
 L-tyrosine pH 7, 37°C Euglena gracilis
2.8.2.9 0.08
-
 L-tyrosine tert-butyl ester pH 7, 37°C Euglena gracilis
2.8.2.9 0.1
-
 2-naphthol L-tyrosine ethyl ester, pH 7, 37°C Euglena gracilis
2.8.2.9 0.133
-
 L-tyrosine benzyl ester pH 7, 37°C Euglena gracilis
2.8.2.9 0.15
-
 L-tyrosine methyl ester pH 7, 37°C Euglena gracilis
2.8.2.9 0.2
-
 3-methoxy-4-hydroxyphenylglycol L-tyrosinamide, pH 7, 37°C Euglena gracilis
2.8.2.9 0.35
-
 AlaTyr pH 7, 37°C Euglena gracilis
2.8.2.9 0.37
-
 L-tyrosine ethyl ester pH 7, 37°C Euglena gracilis
2.8.2.9 0.39 0.4 3-chlorophenol pH 7, 37°C Euglena gracilis
2.8.2.9 0.416
-
 3-Nitrophenol pH 7, 37°C Euglena gracilis
2.8.2.9 0.5
-
 2-chlorophenol pH 7, 37°C Euglena gracilis
2.8.2.9 0.55
-
 4-Chlorophenol pH 7, 37°C Euglena gracilis
2.8.2.9 0.714
-
 L-4-hydroxyphenylglycine pH 7, 37°C Euglena gracilis
2.8.2.9 0.833
-
 Tyr-Gly pH 7, 37°C Euglena gracilis
2.8.2.9 1.25
-
 4-nitrophenol pH 7, 37°C Euglena gracilis
2.8.2.9 2.5
-
 GlyTyr pH 7, 37°C Euglena gracilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.2.9 26000
-
 gel filtration Euglena gracilis

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.1 Euglena gracilis
-
-
-
2.8.2.9 Euglena gracilis
-
 var. bacillaris, W10BSmL, aplastidic mutant
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.1 6416fold Euglena gracilis
2.8.2.9
-
 Euglena gracilis

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.2.1 0.205
-
 purified enzyme Euglena gracilis
2.8.2.9 0.2053
-
-
 Euglena gracilis

Storage Stability

EC Number Storage Stability Organism
2.8.2.9 4°C, 10 mM potassium phosphate, pH 6.8, 250 mM sucrose and 0.02% w/v NaN3, no more than 10% loss of activity after 5 months Euglena gracilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.1 3'-phosphoadenylyl sulfate + a phenol
-
 Euglena gracilis adenosine 3',5'-bisphosphate + an aryl sulfate
-
 ?
2.8.2.1 3'-phosphoadenylylsulfate + 2-chlorophenol
-
 Euglena gracilis adenosine 3',5'-bisphosphate + 2-chlorophenyl sulfate
-
 ?
2.8.2.1 3'-phosphoadenylylsulfate + 2-nitrophenol
-
 Euglena gracilis adenosine 3',5'-bisphosphate + 2-nitrophenyl sulfate
-
 ?
2.8.2.1 3'-phosphoadenylylsulfate + 3-chlorophenol
-
 Euglena gracilis adenosine 3',5'-bisphosphate + 3-chlorophenyl sulfate
-
 ?
2.8.2.1 3'-phosphoadenylylsulfate + 3-nitrophenol
-
 Euglena gracilis adenosine 3',5'-bisphosphate + 3-nitrophenyl sulfate
-
 ?
2.8.2.1 3'-phosphoadenylylsulfate + 4-chlorophenol
-
 Euglena gracilis adenosine 3',5'-bisphosphate + 4-chlorophenyl sulfate
-
 ?
2.8.2.1 3'-phosphoadenylylsulfate + 4-nitrophenol
-
 Euglena gracilis adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate
-
 ?
2.8.2.1 3'-phosphoadenylylsulfate + D-tyrosine no activity with Euglena gracilis adenosine 3',5'-bisphosphate + D-tyrosyl sulfate
-
 ?
2.8.2.1 3'-phosphoadenylylsulfate + L-4-hydroxyphenylglycine no activity with the D-isomer Euglena gracilis adenosine 3',5'-bisphosphate + (2S)-amino[4-(sulfooxy)phenyl]ethanoic acid
-
 ?
2.8.2.1 3'-phosphoadenylylsulfate + L-tyrosine
-
 Euglena gracilis adenosine 3',5'-bisphosphate + L-tyrosyl O4-sulfate
-
 r
2.8.2.1 additional information substrate specificity Euglena gracilis ?
-
 ?
2.8.2.9 3'-phosphoadenylylsulfate + 2-chlorophenol
-
 Euglena gracilis adenosine 3',5'-bisphosphate + 2-chlorophenyl sulfate
-
 ?
2.8.2.9 3'-phosphoadenylylsulfate + 2-naphthol
-
 Euglena gracilis adenosine 3',5'-bisphosphate + 2-naphthyl sulfate
-
 ?
2.8.2.9 3'-phosphoadenylylsulfate + 3-chlorophenol
-
 Euglena gracilis adenosine 3',5'-bisphosphate + 3-chlorophenyl sulfate
-
 ?
2.8.2.9 3'-phosphoadenylylsulfate + 3-methoxy-4-hydroxyphenylglycol
-
 Euglena gracilis adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.9 3'-phosphoadenylylsulfate + 3-nitrophenol
-
 Euglena gracilis adenosine 3',5'-bisphosphate + 3-nitrophenyl sulfate
-
 ?
2.8.2.9 3'-phosphoadenylylsulfate + 4-chlorophenol
-
 Euglena gracilis adenosine 3',5'-bisphosphate + 4-chlorophenyl sulfate
-
 ?
2.8.2.9 3'-phosphoadenylylsulfate + 4-nitrophenol
-
 Euglena gracilis adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate
-
 ?
2.8.2.9 3'-phosphoadenylylsulfate + Ala-Tyr
-
 Euglena gracilis adenosine 3',5'-bisphosphate + Ala-(O-sulfo)Tyr
-
 ?
2.8.2.9 3'-phosphoadenylylsulfate + GlyTyr
-
 Euglena gracilis adenosine 3',5'-bisphosphate + Gly-(O-sulfo)Tyr
-
 ?
2.8.2.9 3'-phosphoadenylylsulfate + L-4-hydroxyphenylglycine
-
 Euglena gracilis adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.9 3'-phosphoadenylylsulfate + L-tyrosine
-
 Euglena gracilis adenosine 3',5'-bisphosphate + tyrosine O4-sulfate
-
 ?
2.8.2.9 3'-phosphoadenylylsulfate + L-tyrosine allyl ester
-
 Euglena gracilis adenosine 3',5'-bisphosphate + tyrosine allyl ester 4-sulfate
-
 ?
2.8.2.9 3'-phosphoadenylylsulfate + L-tyrosine amide
-
 Euglena gracilis adenosine 3',5'-bisphosphate + O-sulfo-L-tyrosine amide
-
 ?
2.8.2.9 3'-phosphoadenylylsulfate + L-tyrosine benzyl ester
-
 Euglena gracilis adenosine 3',5'-bisphosphate + tyrosine benzyl ester 4-sulfate
-
 ?
2.8.2.9 3'-phosphoadenylylsulfate + L-tyrosine ethyl ester
-
 Euglena gracilis adenosine 3',5'-bisphosphate + tyrosine ethyl ester 4-sulfate
-
 ?
2.8.2.9 3'-phosphoadenylylsulfate + L-tyrosine methyl ester
-
 Euglena gracilis adenosine 3',5'-bisphosphate + L-tyrosine methyl ester 4-sulfate
-
 ?
2.8.2.9 3'-phosphoadenylylsulfate + L-tyrosine tert-butyl ester
-
 Euglena gracilis adenosine 3',5'-bisphosphate + tyrosine tert-butyl ester 4-sulfate
-
 ?
2.8.2.9 3'-phosphoadenylylsulfate + nitrophenol 3-nitrophenol or 4-nitrophenol Euglena gracilis adenosine 3',5'-bisphosphate + nitrophenyl sulfate
-
 ?
2.8.2.9 3'-phosphoadenylylsulfate + TyrGly
-
 Euglena gracilis adenosine 3',5'-bisphosphate + 4O-sulfo-Tyr-Gly
-
 ?
2.8.2.9 additional information not: adenosine 5'-phosphosulfate Euglena gracilis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.2.9 monomer 1 * 26000, SDS-PAGE Euglena gracilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.2.1 37
-
 assay at Euglena gracilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.1 7
-
-
 Euglena gracilis
2.8.2.9 7
-
-
 Euglena gracilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.8.2.9 additional information
-
-
 Euglena gracilis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.8.2.1 0.02
-
 adenosine 3',5'-bisphosphate pH 7.0, 37°C Euglena gracilis
2.8.2.1 0.5
-
 L-tyrosine O4-sulfate pH 7.0, 37°C Euglena gracilis
2.8.2.9 0.02
-
 adenosine 3',5'-bisphosphate pH 7, 37°C Euglena gracilis
2.8.2.9 0.5
-
 Tyrosine O4-sulfate pH 7, 37°C Euglena gracilis