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Literature summary extracted from

  • Harayama, S.; Rekik, M.
    The meta cleavage operon of TOL degradative plasmid pWW0 comprises 13 genes (1990), Mol. Gen. Genet., 221, 113-120.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.25 expression of xylL gene in Escherichia coli strain from TOL plasmid Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
1.3.1.25 additional information operon insertion mutants of recombinant Escherichia coli expressing the TOL plasmid encoded enzymes including 1,2-dihydroxy--3,5-cyclohexadiene-1-carboxylate dehydrogenase, sequence analysis Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.25 28000
-
 ? * 28000, DNA sequence determination, recombinant from Escherichia coli Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.25 (-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+ Pseudomonas putida
-
 catechol + CO2 + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.25 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.25 recombinant from Escherichia coli Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.25 (-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+
-
 Pseudomonas putida catechol + CO2 + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.25 ? ? * 28000, DNA sequence determination, recombinant from Escherichia coli Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.25 NAD+
-
 Pseudomonas putida