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Literature summary extracted from

  • Reiner, A.M.
    Metabolism of aromatic compounds in bacteria. Purification and properties of the catechol-forming enzyme, 3,5-cyclohexadiene-1,2-diol-1-carboxylic acid (NAD +) oxidoreductase (decarboxylating) (1972), J. Biol. Chem., 247, 4960-4965.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.25 (NH4)2SO4
-
 Cupriavidus necator
1.3.1.25 MgSO4
-
 Cupriavidus necator
1.3.1.25 Na2SO4 depending on pH Cupriavidus necator
1.3.1.25 NaNO2
-
 Cupriavidus necator
1.3.1.25 NaNO3
-
 Cupriavidus necator
1.3.1.25 NH4Cl
-
 Cupriavidus necator

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.25 additional information
-
 additional information dependency on pH Cupriavidus necator
1.3.1.25 0.15
-
 NAD+ pH 8.0 Cupriavidus necator
1.3.1.25 0.2
-
 (-)-3,5-cyclohexadien-1,2-diol-1-carboxylate
-
 Cupriavidus necator

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.25 24000
-
 ? * 24000, SDS-PAGE Cupriavidus necator
1.3.1.25 94600
-
 sedimentation equilibrium centrifugation Cupriavidus necator

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.25 (-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+ Cupriavidus necator
-
 catechol + CO2 + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.25 Cupriavidus necator
-
 basonym Alcaligenes eutrophus
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.25
-
 Cupriavidus necator

Reaction

EC Number Reaction Comment Organism Reaction ID
1.3.1.25 (1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+ = catechol + CO2 + NADH + H+ mechanism Cupriavidus necator
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.25 150
-
 purified enzyme Cupriavidus necator

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.25 (-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+
-
 Cupriavidus necator catechol + CO2 + NADH
-
 ?
1.3.1.25 additional information no substrates: benzoic acid, cis-3,5-cyclohexadien-1,2-diol Cupriavidus necator ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.25 ? ? * 24000, SDS-PAGE Cupriavidus necator

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.25 25
-
 assay at Cupriavidus necator

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.25 8
-
 assay at Cupriavidus necator

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.25 NAD+ specific for Cupriavidus necator