BRENDA - Enzyme Database show

Indolepyruvate ferredoxin oxidoreductase from the hyperthermophilic archaeon Pyrococcus furiosus. A new enzyme involved in peptide fermentation

Mai, X.; Adams, M.W.; J. Biol. Chem. 269, 16726-16732 (1994)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.2.7.8
thiamine diphosphate
activity increases about 8fold, Km for thiamine diphosphate 0.004 mM
Pyrococcus furiosus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.7.8
Cu2+
-
Pyrococcus furiosus
1.2.7.8
cyanide
-
Pyrococcus furiosus
1.2.7.8
KCN
-
Pyrococcus furiosus
1.2.7.8
additional information
not inhibited by CO, unaffected by sodium nitrite, not inhibited by sodium fluoride or sodium azide, sodium benzoate, phenylacetate, pyruvate, 2-ketoglutarate and oxamate have no effect on activity
Pyrococcus furiosus
1.2.7.8
Zn2+
-
Pyrococcus furiosus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.7.8
0.017
-
CoA
pH 8.4, 80°C, substrate indolepyruvate
Pyrococcus furiosus
1.2.7.8
0.021
-
CoA
pH 8.4, 80°C, substrate phenylpyruvate
Pyrococcus furiosus
1.2.7.8
0.048
-
Ferredoxin
pH 8.4, 80°C
Pyrococcus furiosus
1.2.7.8
0.095
-
phenylpyruvate
pH 8.4, 80°C
Pyrococcus furiosus
1.2.7.8
0.11
-
p-hydroxyphenylpyruvate
pH 8.4, 80°C
Pyrococcus furiosus
1.2.7.8
0.25
-
Indolepyruvate
pH 8.4, 80°C
Pyrococcus furiosus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.2.7.8
cytoplasm
-
Pyrococcus furiosus
5737
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.7.8
23000
-
2 * 66000 + 2 * 23000, alpha2,beta2, SDS-PAGE
Pyrococcus furiosus
1.2.7.8
66000
-
2 * 66000 + 2 * 23000, alpha2,beta2, SDS-PAGE
Pyrococcus furiosus
1.2.7.8
180000
-
gel filtration
Pyrococcus furiosus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.7.8
(indol-3-yl)pyruvate + CoA + oxidized ferredoxin
Pyrococcus furiosus
involved in peptide fermentation and metabolism of aromatic amino acids
S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin
-
-
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.7.8
Pyrococcus furiosus
-
DSM 3638
-
Oxidation Stability
EC Number
Oxidation Stability
Organism
1.2.7.8
enzyme is sensitive to inactivation by O2, losing 50% of its activity after exposure to air for 20 min at 23°C
Pyrococcus furiosus
Purification (Commentary)
EC Number
Commentary
Organism
1.2.7.8
-
Pyrococcus furiosus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2.7.8
38
-
-
Pyrococcus furiosus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.7.8
(indol-3-yl)pyruvate + CoA + oxidized ferredoxin
-
644700
Pyrococcus furiosus
S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin
-
-
-
r
1.2.7.8
(indol-3-yl)pyruvate + CoA + oxidized ferredoxin
involved in peptide fermentation and metabolism of aromatic amino acids
644700
Pyrococcus furiosus
S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin
-
-
-
r
1.2.7.8
2-keto-4-methylthiobutyrate + CoA + oxidized ferredoxin
-
644700
Pyrococcus furiosus
CO2 + reduced ferredoxin + 3-methylthiopropionyl-CoA
-
-
-
r
1.2.7.8
2-ketoisocaproate + CoA + oxidized ferredoxin
-
644700
Pyrococcus furiosus
CO2 + reduced ferredoxin + isovaleryl-CoA
-
-
-
r
1.2.7.8
additional information
pyruvate and transaminated forms of the amino acids aspartate, valine, glutamate and alanine, 2-ketobutyrate, oxalacetate, 2-ketoisovalerate, 2-ketoglutarate, 2-ketomalonate and phenylglyoxylate are not oxidized
644700
Pyrococcus furiosus
?
-
-
-
-
1.2.7.8
p-hydroxyphenylpyruvate + CoA + oxidized ferredoxin
-
644700
Pyrococcus furiosus
p-hydroxyphenylacetyl-CoA + CO2 + reduced ferredoxin
-
-
-
r
1.2.7.8
phenylpyruvate + CoA + oxidized ferredoxin
-
644700
Pyrococcus furiosus
phenylacetyl-CoA + CO2 + reduced ferredoxin
-
-
-
r
Subunits
EC Number
Subunits
Commentary
Organism
1.2.7.8
tetramer
2 * 66000 + 2 * 23000, alpha2,beta2, SDS-PAGE
Pyrococcus furiosus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.2.7.8
90
-
exhibits optimal activity above at pH 8.0
Pyrococcus furiosus
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.2.7.8
70
90
shows dramatic increase in activity above 70°C
Pyrococcus furiosus
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.2.7.8
80
-
virtually inactive at 25°C, quite thermostable, with a half-life of activity at 80°C of about 80 min under anaerobic conditions
Pyrococcus furiosus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.7.8
8.5
10.5
at 80°C
Pyrococcus furiosus
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.2.7.8
8
10
not active at pH 5.2 and pH 11.0
Pyrococcus furiosus
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.2.7.8
7.5
-
KCN
pH 8.4, 80°C
Pyrococcus furiosus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.2.7.8
thiamine diphosphate
activity increases about 8fold, Km for thiamine diphosphate 0.004 mM
Pyrococcus furiosus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.7.8
Cu2+
-
Pyrococcus furiosus
1.2.7.8
cyanide
-
Pyrococcus furiosus
1.2.7.8
KCN
-
Pyrococcus furiosus
1.2.7.8
additional information
not inhibited by CO, unaffected by sodium nitrite, not inhibited by sodium fluoride or sodium azide, sodium benzoate, phenylacetate, pyruvate, 2-ketoglutarate and oxamate have no effect on activity
Pyrococcus furiosus
1.2.7.8
Zn2+
-
Pyrococcus furiosus
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.2.7.8
7.5
-
KCN
pH 8.4, 80°C
Pyrococcus furiosus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.7.8
0.017
-
CoA
pH 8.4, 80°C, substrate indolepyruvate
Pyrococcus furiosus
1.2.7.8
0.021
-
CoA
pH 8.4, 80°C, substrate phenylpyruvate
Pyrococcus furiosus
1.2.7.8
0.048
-
Ferredoxin
pH 8.4, 80°C
Pyrococcus furiosus
1.2.7.8
0.095
-
phenylpyruvate
pH 8.4, 80°C
Pyrococcus furiosus
1.2.7.8
0.11
-
p-hydroxyphenylpyruvate
pH 8.4, 80°C
Pyrococcus furiosus
1.2.7.8
0.25
-
Indolepyruvate
pH 8.4, 80°C
Pyrococcus furiosus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.2.7.8
cytoplasm
-
Pyrococcus furiosus
5737
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.7.8
23000
-
2 * 66000 + 2 * 23000, alpha2,beta2, SDS-PAGE
Pyrococcus furiosus
1.2.7.8
66000
-
2 * 66000 + 2 * 23000, alpha2,beta2, SDS-PAGE
Pyrococcus furiosus
1.2.7.8
180000
-
gel filtration
Pyrococcus furiosus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.7.8
(indol-3-yl)pyruvate + CoA + oxidized ferredoxin
Pyrococcus furiosus
involved in peptide fermentation and metabolism of aromatic amino acids
S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin
-
-
r
Oxidation Stability (protein specific)
EC Number
Oxidation Stability
Organism
1.2.7.8
enzyme is sensitive to inactivation by O2, losing 50% of its activity after exposure to air for 20 min at 23°C
Pyrococcus furiosus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.7.8
-
Pyrococcus furiosus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2.7.8
38
-
-
Pyrococcus furiosus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.7.8
(indol-3-yl)pyruvate + CoA + oxidized ferredoxin
-
644700
Pyrococcus furiosus
S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin
-
-
-
r
1.2.7.8
(indol-3-yl)pyruvate + CoA + oxidized ferredoxin
involved in peptide fermentation and metabolism of aromatic amino acids
644700
Pyrococcus furiosus
S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin
-
-
-
r
1.2.7.8
2-keto-4-methylthiobutyrate + CoA + oxidized ferredoxin
-
644700
Pyrococcus furiosus
CO2 + reduced ferredoxin + 3-methylthiopropionyl-CoA
-
-
-
r
1.2.7.8
2-ketoisocaproate + CoA + oxidized ferredoxin
-
644700
Pyrococcus furiosus
CO2 + reduced ferredoxin + isovaleryl-CoA
-
-
-
r
1.2.7.8
additional information
pyruvate and transaminated forms of the amino acids aspartate, valine, glutamate and alanine, 2-ketobutyrate, oxalacetate, 2-ketoisovalerate, 2-ketoglutarate, 2-ketomalonate and phenylglyoxylate are not oxidized
644700
Pyrococcus furiosus
?
-
-
-
-
1.2.7.8
p-hydroxyphenylpyruvate + CoA + oxidized ferredoxin
-
644700
Pyrococcus furiosus
p-hydroxyphenylacetyl-CoA + CO2 + reduced ferredoxin
-
-
-
r
1.2.7.8
phenylpyruvate + CoA + oxidized ferredoxin
-
644700
Pyrococcus furiosus
phenylacetyl-CoA + CO2 + reduced ferredoxin
-
-
-
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.2.7.8
tetramer
2 * 66000 + 2 * 23000, alpha2,beta2, SDS-PAGE
Pyrococcus furiosus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.2.7.8
90
-
exhibits optimal activity above at pH 8.0
Pyrococcus furiosus
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.2.7.8
70
90
shows dramatic increase in activity above 70°C
Pyrococcus furiosus
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.2.7.8
80
-
virtually inactive at 25°C, quite thermostable, with a half-life of activity at 80°C of about 80 min under anaerobic conditions
Pyrococcus furiosus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.7.8
8.5
10.5
at 80°C
Pyrococcus furiosus
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.2.7.8
8
10
not active at pH 5.2 and pH 11.0
Pyrococcus furiosus