BRENDA - Enzyme Database show

Structures and functions of four anabolic 2-oxoacid oxidoreductases in Methanobacterium thermoautotrophicum

Tersteegen, A.; Linder, D.; Thauer, R.K.; Hedderich, R.; Eur. J. Biochem. 244, 862-868 (1997)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.7.7
0.004
-
CoA
pH 8.0, 65°C, anaerobic
Methanothermobacter thermautotrophicus
1.2.7.7
1.3
-
2-ketoisovalerate
pH 8.0, 65°C, anaerobic
Methanothermobacter thermautotrophicus
1.2.7.8
0.02
-
CoA
pH 8.0, 65°C, substrate phenylpyruvate, benzyl viologen as electron acceptor
Methanothermobacter thermautotrophicus
1.2.7.8
1.4
-
phenylpyruvate
pH 8.0, 65°C
Methanothermobacter thermautotrophicus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.7.7
15000
-
x * 55000 + x * 37000 + x * 15000, SDS-PAGE
Methanothermobacter thermautotrophicus
1.2.7.7
37000
-
x * 55000 + x * 37000 + x * 15000, SDS-PAGE
Methanothermobacter thermautotrophicus
1.2.7.7
55000
-
x * 55000 + x * 37000 + x * 15000, SDS-PAGE
Methanothermobacter thermautotrophicus
1.2.7.8
23000
-
2 * 67000 + 2 * 23000, alpha2,beta2, SDS-PAGE
Methanothermobacter thermautotrophicus
1.2.7.8
67000
-
2 * 67000 + 2 * 23000, alpha2,beta2, SDS-PAGE
Methanothermobacter thermautotrophicus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.7.7
additional information
Pyrococcus furiosus
-
?
-
-
-
1.2.7.7
additional information
Thermococcus litoralis
-
?
-
-
-
1.2.7.7
additional information
Methanothermobacter thermautotrophicus
enzyme is involved in the biosynthesis of amino acids from fatty acids take up from the grown medium
?
-
-
-
1.2.7.7
additional information
Methanothermobacter thermautotrophicus Marburg / DSM 2133
enzyme is involved in the biosynthesis of amino acids from fatty acids take up from the grown medium
?
-
-
-
1.2.7.8
(indol-3-yl)pyruvate + CoA + oxidized ferredoxin
Methanothermobacter thermautotrophicus
biosynthesis of amino acids from fatty acids
S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin
-
-
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.7.7
Methanothermobacter thermautotrophicus
-
strain DSM 2133
-
1.2.7.7
Pyrococcus furiosus
-
-
-
1.2.7.7
Thermococcus litoralis
-
-
-
1.2.7.8
Methanothermobacter thermautotrophicus
-
-
-
1.2.7.8
Methanothermobacter thermautotrophicus Marburg / DSM 2133
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.2.7.7
under anaerobic conditions
Methanothermobacter thermautotrophicus
1.2.7.8
-
Methanothermobacter thermautotrophicus
Reaction
EC Number
Reaction
Commentary
Organism
1.2.7.7
3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin = S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + H+
this enzyme is one of four 2-oxoacid oxidoreductases: indolepyruvate oxidoreductase, EC 1.2.7.8, pyruvate oxidoreductase, EC 1.2.7.1., 2-oxogluterate oxidoreductase, EC 1.2.7.3
Methanothermobacter thermautotrophicus
1.2.7.7
3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin = S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + H+
this enzyme is one of four 2-oxoacid oxidoreductases: indolepyruvate oxidoreductase, EC 1.2.7.8, pyruvate oxidoreductase, EC 1.2.7.1., 2-oxogluterate oxidoreductase, EC 1.2.7.3
Pyrococcus furiosus
1.2.7.7
3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin = S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + H+
this enzyme is one of four 2-oxoacid oxidoreductases: indolepyruvate oxidoreductase, EC 1.2.7.8, pyruvate oxidoreductase, EC 1.2.7.1., 2-oxogluterate oxidoreductase, EC 1.2.7.3
Thermococcus litoralis
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2.7.7
13.8
-
-
Methanothermobacter thermautotrophicus
1.2.7.8
31.15
-
-
Methanothermobacter thermautotrophicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.7.7
2-ketobutyrate + CoA + oxidized ferredoxin
-
644697
Pyrococcus furiosus
propanoyl-CoA + CO2 + reduced ferredoxin
-
-
-
?
1.2.7.7
2-ketobutyrate + CoA + oxidized ferredoxin
-
644697
Thermococcus litoralis
propanoyl-CoA + CO2 + reduced ferredoxin
-
-
-
?
1.2.7.7
2-ketobutyrate + CoA + oxidized ferredoxin
benzyl viologen as electron acceptor
644697
Methanothermobacter thermautotrophicus
propanoyl-CoA + CO2 + reduced ferredoxin
-
-
-
?
1.2.7.7
2-ketobutyrate + CoA + oxidized ferredoxin
benzyl viologen as electron acceptor
644697
Methanothermobacter thermautotrophicus Marburg / DSM 2133
propanoyl-CoA + CO2 + reduced ferredoxin
-
-
-
?
1.2.7.7
2-ketoisohexanoate + CoA + benzyl viologen
-
644697
Methanothermobacter thermautotrophicus
isopentanoyl-CoA + CO2 + reduced benzyl viologen
-
-
-
?
1.2.7.7
2-ketoisovalerate + CoA + benzyl viologen
-
644697
Methanothermobacter thermautotrophicus
S-(2-methylpropanoyl)-CoA + CO2 + reduced benzyl viologen
-
-
-
-
1.2.7.7
2-ketoisovalerate + CoA + benzyl viologen
-
644697
Methanothermobacter thermautotrophicus Marburg / DSM 2133
S-(2-methylpropanoyl)-CoA + CO2 + reduced benzyl viologen
-
-
-
-
1.2.7.7
2-ketovalerate + CoA + benzyl viologen
-
644697
Methanothermobacter thermautotrophicus
butanoyl-CoA + CO2 + reduced benzyl viologen
-
-
-
?
1.2.7.7
additional information
-
644697
Pyrococcus furiosus
?
-
-
-
-
1.2.7.7
additional information
-
644697
Thermococcus litoralis
?
-
-
-
-
1.2.7.7
additional information
no substrate are 2-ketoglutarate and pyruvate
644697
Methanothermobacter thermautotrophicus
?
-
-
-
-
1.2.7.7
additional information
enzyme is involved in the biosynthesis of amino acids from fatty acids take up from the grown medium
644697
Methanothermobacter thermautotrophicus
?
-
-
-
-
1.2.7.7
additional information
no substrate are 2-ketoglutarate and pyruvate
644697
Methanothermobacter thermautotrophicus Marburg / DSM 2133
?
-
-
-
-
1.2.7.7
additional information
enzyme is involved in the biosynthesis of amino acids from fatty acids take up from the grown medium
644697
Methanothermobacter thermautotrophicus Marburg / DSM 2133
?
-
-
-
-
1.2.7.7
phenylpyruvate + CoA + oxidized ferredoxin
-
644697
Pyrococcus furiosus
phenylacetyl-CoA + CO2 + reduced ferredoxin
-
-
-
?
1.2.7.7
phenylpyruvate + CoA + oxidized ferredoxin
-
644697
Thermococcus litoralis
phenylacetyl-CoA + CO2 + reduced ferredoxin
-
-
-
?
1.2.7.7
phenylpyruvate + CoA + oxidized ferredoxin
benzyl viologen as electron acceptor
644697
Methanothermobacter thermautotrophicus
phenylacetyl-CoA + CO2 + reduced ferredoxin
-
-
-
?
1.2.7.7
phenylpyruvate + CoA + oxidized ferredoxin
benzyl viologen as electron acceptor
644697
Methanothermobacter thermautotrophicus Marburg / DSM 2133
phenylacetyl-CoA + CO2 + reduced ferredoxin
-
-
-
?
1.2.7.8
(indol-3-yl)pyruvate + CoA + oxidized ferredoxin
-
644697
Methanothermobacter thermautotrophicus
S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin
-
-
-
r
1.2.7.8
(indol-3-yl)pyruvate + CoA + oxidized ferredoxin
biosynthesis of amino acids from fatty acids
644697
Methanothermobacter thermautotrophicus
S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin
-
-
-
r
1.2.7.8
phenylpyruvate + CoA + oxidized ferredoxin
-
644697
Methanothermobacter thermautotrophicus
phenylacetyl-CoA + CO2 + reduced ferredoxin
-
-
-
r
Subunits
EC Number
Subunits
Commentary
Organism
1.2.7.7
?
comparison of N-terminal amino acid sequences; x * 55000 + x * 37000 + x * 15000, SDS-PAGE
Methanothermobacter thermautotrophicus
1.2.7.7
?
comparison of N-terminal amino acid sequences
Pyrococcus furiosus
1.2.7.7
?
comparison of N-terminal amino acid sequences
Thermococcus litoralis
1.2.7.8
tetramer
2 * 67000 + 2 * 23000, alpha2,beta2, SDS-PAGE
Methanothermobacter thermautotrophicus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.2.7.7
75
-
at pH 8.0
Methanothermobacter thermautotrophicus
1.2.7.8
80
-
-
Methanothermobacter thermautotrophicus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.7.7
9.7
-
at 65°C
Methanothermobacter thermautotrophicus
1.2.7.8
10
-
-
Methanothermobacter thermautotrophicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.7.7
0.004
-
CoA
pH 8.0, 65°C, anaerobic
Methanothermobacter thermautotrophicus
1.2.7.7
1.3
-
2-ketoisovalerate
pH 8.0, 65°C, anaerobic
Methanothermobacter thermautotrophicus
1.2.7.8
0.02
-
CoA
pH 8.0, 65°C, substrate phenylpyruvate, benzyl viologen as electron acceptor
Methanothermobacter thermautotrophicus
1.2.7.8
1.4
-
phenylpyruvate
pH 8.0, 65°C
Methanothermobacter thermautotrophicus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.7.7
15000
-
x * 55000 + x * 37000 + x * 15000, SDS-PAGE
Methanothermobacter thermautotrophicus
1.2.7.7
37000
-
x * 55000 + x * 37000 + x * 15000, SDS-PAGE
Methanothermobacter thermautotrophicus
1.2.7.7
55000
-
x * 55000 + x * 37000 + x * 15000, SDS-PAGE
Methanothermobacter thermautotrophicus
1.2.7.8
23000
-
2 * 67000 + 2 * 23000, alpha2,beta2, SDS-PAGE
Methanothermobacter thermautotrophicus
1.2.7.8
67000
-
2 * 67000 + 2 * 23000, alpha2,beta2, SDS-PAGE
Methanothermobacter thermautotrophicus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.7.7
additional information
Pyrococcus furiosus
-
?
-
-
-
1.2.7.7
additional information
Thermococcus litoralis
-
?
-
-
-
1.2.7.7
additional information
Methanothermobacter thermautotrophicus
enzyme is involved in the biosynthesis of amino acids from fatty acids take up from the grown medium
?
-
-
-
1.2.7.7
additional information
Methanothermobacter thermautotrophicus Marburg / DSM 2133
enzyme is involved in the biosynthesis of amino acids from fatty acids take up from the grown medium
?
-
-
-
1.2.7.8
(indol-3-yl)pyruvate + CoA + oxidized ferredoxin
Methanothermobacter thermautotrophicus
biosynthesis of amino acids from fatty acids
S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin
-
-
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.7.7
under anaerobic conditions
Methanothermobacter thermautotrophicus
1.2.7.8
-
Methanothermobacter thermautotrophicus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2.7.7
13.8
-
-
Methanothermobacter thermautotrophicus
1.2.7.8
31.15
-
-
Methanothermobacter thermautotrophicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.7.7
2-ketobutyrate + CoA + oxidized ferredoxin
-
644697
Pyrococcus furiosus
propanoyl-CoA + CO2 + reduced ferredoxin
-
-
-
?
1.2.7.7
2-ketobutyrate + CoA + oxidized ferredoxin
-
644697
Thermococcus litoralis
propanoyl-CoA + CO2 + reduced ferredoxin
-
-
-
?
1.2.7.7
2-ketobutyrate + CoA + oxidized ferredoxin
benzyl viologen as electron acceptor
644697
Methanothermobacter thermautotrophicus
propanoyl-CoA + CO2 + reduced ferredoxin
-
-
-
?
1.2.7.7
2-ketobutyrate + CoA + oxidized ferredoxin
benzyl viologen as electron acceptor
644697
Methanothermobacter thermautotrophicus Marburg / DSM 2133
propanoyl-CoA + CO2 + reduced ferredoxin
-
-
-
?
1.2.7.7
2-ketoisohexanoate + CoA + benzyl viologen
-
644697
Methanothermobacter thermautotrophicus
isopentanoyl-CoA + CO2 + reduced benzyl viologen
-
-
-
?
1.2.7.7
2-ketoisovalerate + CoA + benzyl viologen
-
644697
Methanothermobacter thermautotrophicus
S-(2-methylpropanoyl)-CoA + CO2 + reduced benzyl viologen
-
-
-
-
1.2.7.7
2-ketoisovalerate + CoA + benzyl viologen
-
644697
Methanothermobacter thermautotrophicus Marburg / DSM 2133
S-(2-methylpropanoyl)-CoA + CO2 + reduced benzyl viologen
-
-
-
-
1.2.7.7
2-ketovalerate + CoA + benzyl viologen
-
644697
Methanothermobacter thermautotrophicus
butanoyl-CoA + CO2 + reduced benzyl viologen
-
-
-
?
1.2.7.7
additional information
-
644697
Pyrococcus furiosus
?
-
-
-
-
1.2.7.7
additional information
-
644697
Thermococcus litoralis
?
-
-
-
-
1.2.7.7
additional information
no substrate are 2-ketoglutarate and pyruvate
644697
Methanothermobacter thermautotrophicus
?
-
-
-
-
1.2.7.7
additional information
enzyme is involved in the biosynthesis of amino acids from fatty acids take up from the grown medium
644697
Methanothermobacter thermautotrophicus
?
-
-
-
-
1.2.7.7
additional information
no substrate are 2-ketoglutarate and pyruvate
644697
Methanothermobacter thermautotrophicus Marburg / DSM 2133
?
-
-
-
-
1.2.7.7
additional information
enzyme is involved in the biosynthesis of amino acids from fatty acids take up from the grown medium
644697
Methanothermobacter thermautotrophicus Marburg / DSM 2133
?
-
-
-
-
1.2.7.7
phenylpyruvate + CoA + oxidized ferredoxin
-
644697
Pyrococcus furiosus
phenylacetyl-CoA + CO2 + reduced ferredoxin
-
-
-
?
1.2.7.7
phenylpyruvate + CoA + oxidized ferredoxin
-
644697
Thermococcus litoralis
phenylacetyl-CoA + CO2 + reduced ferredoxin
-
-
-
?
1.2.7.7
phenylpyruvate + CoA + oxidized ferredoxin
benzyl viologen as electron acceptor
644697
Methanothermobacter thermautotrophicus
phenylacetyl-CoA + CO2 + reduced ferredoxin
-
-
-
?
1.2.7.7
phenylpyruvate + CoA + oxidized ferredoxin
benzyl viologen as electron acceptor
644697
Methanothermobacter thermautotrophicus Marburg / DSM 2133
phenylacetyl-CoA + CO2 + reduced ferredoxin
-
-
-
?
1.2.7.8
(indol-3-yl)pyruvate + CoA + oxidized ferredoxin
-
644697
Methanothermobacter thermautotrophicus
S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin
-
-
-
r
1.2.7.8
(indol-3-yl)pyruvate + CoA + oxidized ferredoxin
biosynthesis of amino acids from fatty acids
644697
Methanothermobacter thermautotrophicus
S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin
-
-
-
r
1.2.7.8
phenylpyruvate + CoA + oxidized ferredoxin
-
644697
Methanothermobacter thermautotrophicus
phenylacetyl-CoA + CO2 + reduced ferredoxin
-
-
-
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.2.7.7
?
comparison of N-terminal amino acid sequences; x * 55000 + x * 37000 + x * 15000, SDS-PAGE
Methanothermobacter thermautotrophicus
1.2.7.7
?
comparison of N-terminal amino acid sequences
Pyrococcus furiosus
1.2.7.7
?
comparison of N-terminal amino acid sequences
Thermococcus litoralis
1.2.7.8
tetramer
2 * 67000 + 2 * 23000, alpha2,beta2, SDS-PAGE
Methanothermobacter thermautotrophicus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.2.7.7
75
-
at pH 8.0
Methanothermobacter thermautotrophicus
1.2.7.8
80
-
-
Methanothermobacter thermautotrophicus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.7.7
9.7
-
at 65°C
Methanothermobacter thermautotrophicus
1.2.7.8
10
-
-
Methanothermobacter thermautotrophicus