EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.7.7 | 0.004 | - |
CoA | pH 8.0, 65°C, anaerobic | Methanothermobacter thermautotrophicus | |
1.2.7.7 | 1.3 | - |
2-ketoisovalerate | pH 8.0, 65°C, anaerobic | Methanothermobacter thermautotrophicus | |
1.2.7.8 | 0.02 | - |
CoA | pH 8.0, 65°C, substrate phenylpyruvate, benzyl viologen as electron acceptor | Methanothermobacter thermautotrophicus | |
1.2.7.8 | 1.4 | - |
phenylpyruvate | pH 8.0, 65°C | Methanothermobacter thermautotrophicus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.2.7.7 | 15000 | - |
x * 55000 + x * 37000 + x * 15000, SDS-PAGE | Methanothermobacter thermautotrophicus |
1.2.7.7 | 37000 | - |
x * 55000 + x * 37000 + x * 15000, SDS-PAGE | Methanothermobacter thermautotrophicus |
1.2.7.7 | 55000 | - |
x * 55000 + x * 37000 + x * 15000, SDS-PAGE | Methanothermobacter thermautotrophicus |
1.2.7.8 | 23000 | - |
2 * 67000 + 2 * 23000, alpha2,beta2, SDS-PAGE | Methanothermobacter thermautotrophicus |
1.2.7.8 | 67000 | - |
2 * 67000 + 2 * 23000, alpha2,beta2, SDS-PAGE | Methanothermobacter thermautotrophicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.7.7 | additional information | Pyrococcus furiosus | - |
? | - |
? | |
1.2.7.7 | additional information | Thermococcus litoralis | - |
? | - |
? | |
1.2.7.7 | additional information | Methanothermobacter thermautotrophicus | enzyme is involved in the biosynthesis of amino acids from fatty acids take up from the grown medium | ? | - |
? | |
1.2.7.7 | additional information | Methanothermobacter thermautotrophicus Marburg / DSM 2133 | enzyme is involved in the biosynthesis of amino acids from fatty acids take up from the grown medium | ? | - |
? | |
1.2.7.8 | (indol-3-yl)pyruvate + CoA + oxidized ferredoxin | Methanothermobacter thermautotrophicus | biosynthesis of amino acids from fatty acids | S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.7.7 | Methanothermobacter thermautotrophicus | - |
strain DSM 2133 | - |
1.2.7.7 | Pyrococcus furiosus | - |
- |
- |
1.2.7.7 | Thermococcus litoralis | - |
- |
- |
1.2.7.8 | Methanothermobacter thermautotrophicus | - |
- |
- |
1.2.7.8 | Methanothermobacter thermautotrophicus Marburg / DSM 2133 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.7.7 | under anaerobic conditions | Methanothermobacter thermautotrophicus |
1.2.7.8 | - |
Methanothermobacter thermautotrophicus |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.2.7.7 | 3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin = S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | this enzyme is one of four 2-oxoacid oxidoreductases: indolepyruvate oxidoreductase, EC 1.2.7.8, pyruvate oxidoreductase, EC 1.2.7.1., 2-oxogluterate oxidoreductase, EC 1.2.7.3 | Methanothermobacter thermautotrophicus | |
1.2.7.7 | 3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin = S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | this enzyme is one of four 2-oxoacid oxidoreductases: indolepyruvate oxidoreductase, EC 1.2.7.8, pyruvate oxidoreductase, EC 1.2.7.1., 2-oxogluterate oxidoreductase, EC 1.2.7.3 | Pyrococcus furiosus | |
1.2.7.7 | 3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin = S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | this enzyme is one of four 2-oxoacid oxidoreductases: indolepyruvate oxidoreductase, EC 1.2.7.8, pyruvate oxidoreductase, EC 1.2.7.1., 2-oxogluterate oxidoreductase, EC 1.2.7.3 | Thermococcus litoralis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.2.7.7 | 13.8 | - |
- |
Methanothermobacter thermautotrophicus |
1.2.7.8 | 31.15 | - |
- |
Methanothermobacter thermautotrophicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.7.7 | 2-ketobutyrate + CoA + oxidized ferredoxin | - |
Pyrococcus furiosus | propanoyl-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | 2-ketobutyrate + CoA + oxidized ferredoxin | - |
Thermococcus litoralis | propanoyl-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | 2-ketobutyrate + CoA + oxidized ferredoxin | benzyl viologen as electron acceptor | Methanothermobacter thermautotrophicus | propanoyl-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | 2-ketobutyrate + CoA + oxidized ferredoxin | benzyl viologen as electron acceptor | Methanothermobacter thermautotrophicus Marburg / DSM 2133 | propanoyl-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | 2-ketoisohexanoate + CoA + benzyl viologen | - |
Methanothermobacter thermautotrophicus | isopentanoyl-CoA + CO2 + reduced benzyl viologen | - |
? | |
1.2.7.7 | 2-ketoisovalerate + CoA + benzyl viologen | - |
Methanothermobacter thermautotrophicus | S-(2-methylpropanoyl)-CoA + CO2 + reduced benzyl viologen | - |
? | |
1.2.7.7 | 2-ketoisovalerate + CoA + benzyl viologen | - |
Methanothermobacter thermautotrophicus Marburg / DSM 2133 | S-(2-methylpropanoyl)-CoA + CO2 + reduced benzyl viologen | - |
? | |
1.2.7.7 | 2-ketovalerate + CoA + benzyl viologen | - |
Methanothermobacter thermautotrophicus | butanoyl-CoA + CO2 + reduced benzyl viologen | - |
? | |
1.2.7.7 | additional information | - |
Pyrococcus furiosus | ? | - |
? | |
1.2.7.7 | additional information | - |
Thermococcus litoralis | ? | - |
? | |
1.2.7.7 | additional information | no substrate are 2-ketoglutarate and pyruvate | Methanothermobacter thermautotrophicus | ? | - |
? | |
1.2.7.7 | additional information | enzyme is involved in the biosynthesis of amino acids from fatty acids take up from the grown medium | Methanothermobacter thermautotrophicus | ? | - |
? | |
1.2.7.7 | additional information | no substrate are 2-ketoglutarate and pyruvate | Methanothermobacter thermautotrophicus Marburg / DSM 2133 | ? | - |
? | |
1.2.7.7 | additional information | enzyme is involved in the biosynthesis of amino acids from fatty acids take up from the grown medium | Methanothermobacter thermautotrophicus Marburg / DSM 2133 | ? | - |
? | |
1.2.7.7 | phenylpyruvate + CoA + oxidized ferredoxin | - |
Pyrococcus furiosus | phenylacetyl-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | phenylpyruvate + CoA + oxidized ferredoxin | - |
Thermococcus litoralis | phenylacetyl-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | phenylpyruvate + CoA + oxidized ferredoxin | benzyl viologen as electron acceptor | Methanothermobacter thermautotrophicus | phenylacetyl-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | phenylpyruvate + CoA + oxidized ferredoxin | benzyl viologen as electron acceptor | Methanothermobacter thermautotrophicus Marburg / DSM 2133 | phenylacetyl-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.8 | (indol-3-yl)pyruvate + CoA + oxidized ferredoxin | - |
Methanothermobacter thermautotrophicus | S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin | - |
r | |
1.2.7.8 | (indol-3-yl)pyruvate + CoA + oxidized ferredoxin | biosynthesis of amino acids from fatty acids | Methanothermobacter thermautotrophicus | S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin | - |
r | |
1.2.7.8 | phenylpyruvate + CoA + oxidized ferredoxin | - |
Methanothermobacter thermautotrophicus | phenylacetyl-CoA + CO2 + reduced ferredoxin | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.2.7.7 | ? | x * 55000 + x * 37000 + x * 15000, SDS-PAGE | Methanothermobacter thermautotrophicus |
1.2.7.7 | ? | comparison of N-terminal amino acid sequences | Methanothermobacter thermautotrophicus |
1.2.7.7 | ? | comparison of N-terminal amino acid sequences | Pyrococcus furiosus |
1.2.7.7 | ? | comparison of N-terminal amino acid sequences | Thermococcus litoralis |
1.2.7.8 | tetramer | 2 * 67000 + 2 * 23000, alpha2,beta2, SDS-PAGE | Methanothermobacter thermautotrophicus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.2.7.7 | 75 | - |
at pH 8.0 | Methanothermobacter thermautotrophicus |
1.2.7.8 | 80 | - |
- |
Methanothermobacter thermautotrophicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.2.7.7 | 9.7 | - |
at 65°C | Methanothermobacter thermautotrophicus |
1.2.7.8 | 10 | - |
- |
Methanothermobacter thermautotrophicus |