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Literature summary extracted from

  • Tersteegen, A.; Linder, D.; Thauer, R.K.; Hedderich, R.
    Structures and functions of four anabolic 2-oxoacid oxidoreductases in Methanobacterium thermoautotrophicum (1997), Eur. J. Biochem., 244, 862-868.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.7.7 0.004
-
CoA pH 8.0, 65°C, anaerobic Methanothermobacter thermautotrophicus
1.2.7.7 1.3
-
2-ketoisovalerate pH 8.0, 65°C, anaerobic Methanothermobacter thermautotrophicus
1.2.7.8 0.02
-
CoA pH 8.0, 65°C, substrate phenylpyruvate, benzyl viologen as electron acceptor Methanothermobacter thermautotrophicus
1.2.7.8 1.4
-
phenylpyruvate pH 8.0, 65°C Methanothermobacter thermautotrophicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.7.7 15000
-
x * 55000 + x * 37000 + x * 15000, SDS-PAGE Methanothermobacter thermautotrophicus
1.2.7.7 37000
-
x * 55000 + x * 37000 + x * 15000, SDS-PAGE Methanothermobacter thermautotrophicus
1.2.7.7 55000
-
x * 55000 + x * 37000 + x * 15000, SDS-PAGE Methanothermobacter thermautotrophicus
1.2.7.8 23000
-
2 * 67000 + 2 * 23000, alpha2,beta2, SDS-PAGE Methanothermobacter thermautotrophicus
1.2.7.8 67000
-
2 * 67000 + 2 * 23000, alpha2,beta2, SDS-PAGE Methanothermobacter thermautotrophicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.7.7 additional information Pyrococcus furiosus
-
?
-
?
1.2.7.7 additional information Thermococcus litoralis
-
?
-
?
1.2.7.7 additional information Methanothermobacter thermautotrophicus enzyme is involved in the biosynthesis of amino acids from fatty acids take up from the grown medium ?
-
?
1.2.7.7 additional information Methanothermobacter thermautotrophicus Marburg / DSM 2133 enzyme is involved in the biosynthesis of amino acids from fatty acids take up from the grown medium ?
-
?
1.2.7.8 (indol-3-yl)pyruvate + CoA + oxidized ferredoxin Methanothermobacter thermautotrophicus biosynthesis of amino acids from fatty acids S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin
-
r

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.7 Methanothermobacter thermautotrophicus
-
strain DSM 2133
-
1.2.7.7 Pyrococcus furiosus
-
-
-
1.2.7.7 Thermococcus litoralis
-
-
-
1.2.7.8 Methanothermobacter thermautotrophicus
-
-
-
1.2.7.8 Methanothermobacter thermautotrophicus Marburg / DSM 2133
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.7.7 under anaerobic conditions Methanothermobacter thermautotrophicus
1.2.7.8
-
Methanothermobacter thermautotrophicus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.2.7.7 3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin = S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + 2 H+ this enzyme is one of four 2-oxoacid oxidoreductases: indolepyruvate oxidoreductase, EC 1.2.7.8, pyruvate oxidoreductase, EC 1.2.7.1., 2-oxogluterate oxidoreductase, EC 1.2.7.3 Methanothermobacter thermautotrophicus
1.2.7.7 3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin = S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + 2 H+ this enzyme is one of four 2-oxoacid oxidoreductases: indolepyruvate oxidoreductase, EC 1.2.7.8, pyruvate oxidoreductase, EC 1.2.7.1., 2-oxogluterate oxidoreductase, EC 1.2.7.3 Pyrococcus furiosus
1.2.7.7 3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin = S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + 2 H+ this enzyme is one of four 2-oxoacid oxidoreductases: indolepyruvate oxidoreductase, EC 1.2.7.8, pyruvate oxidoreductase, EC 1.2.7.1., 2-oxogluterate oxidoreductase, EC 1.2.7.3 Thermococcus litoralis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.7.7 13.8
-
-
Methanothermobacter thermautotrophicus
1.2.7.8 31.15
-
-
Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.7 2-ketobutyrate + CoA + oxidized ferredoxin
-
Pyrococcus furiosus propanoyl-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 2-ketobutyrate + CoA + oxidized ferredoxin
-
Thermococcus litoralis propanoyl-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 2-ketobutyrate + CoA + oxidized ferredoxin benzyl viologen as electron acceptor Methanothermobacter thermautotrophicus propanoyl-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 2-ketobutyrate + CoA + oxidized ferredoxin benzyl viologen as electron acceptor Methanothermobacter thermautotrophicus Marburg / DSM 2133 propanoyl-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 2-ketoisohexanoate + CoA + benzyl viologen
-
Methanothermobacter thermautotrophicus isopentanoyl-CoA + CO2 + reduced benzyl viologen
-
?
1.2.7.7 2-ketoisovalerate + CoA + benzyl viologen
-
Methanothermobacter thermautotrophicus S-(2-methylpropanoyl)-CoA + CO2 + reduced benzyl viologen
-
?
1.2.7.7 2-ketoisovalerate + CoA + benzyl viologen
-
Methanothermobacter thermautotrophicus Marburg / DSM 2133 S-(2-methylpropanoyl)-CoA + CO2 + reduced benzyl viologen
-
?
1.2.7.7 2-ketovalerate + CoA + benzyl viologen
-
Methanothermobacter thermautotrophicus butanoyl-CoA + CO2 + reduced benzyl viologen
-
?
1.2.7.7 additional information
-
Pyrococcus furiosus ?
-
?
1.2.7.7 additional information
-
Thermococcus litoralis ?
-
?
1.2.7.7 additional information no substrate are 2-ketoglutarate and pyruvate Methanothermobacter thermautotrophicus ?
-
?
1.2.7.7 additional information enzyme is involved in the biosynthesis of amino acids from fatty acids take up from the grown medium Methanothermobacter thermautotrophicus ?
-
?
1.2.7.7 additional information no substrate are 2-ketoglutarate and pyruvate Methanothermobacter thermautotrophicus Marburg / DSM 2133 ?
-
?
1.2.7.7 additional information enzyme is involved in the biosynthesis of amino acids from fatty acids take up from the grown medium Methanothermobacter thermautotrophicus Marburg / DSM 2133 ?
-
?
1.2.7.7 phenylpyruvate + CoA + oxidized ferredoxin
-
Pyrococcus furiosus phenylacetyl-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 phenylpyruvate + CoA + oxidized ferredoxin
-
Thermococcus litoralis phenylacetyl-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 phenylpyruvate + CoA + oxidized ferredoxin benzyl viologen as electron acceptor Methanothermobacter thermautotrophicus phenylacetyl-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 phenylpyruvate + CoA + oxidized ferredoxin benzyl viologen as electron acceptor Methanothermobacter thermautotrophicus Marburg / DSM 2133 phenylacetyl-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.8 (indol-3-yl)pyruvate + CoA + oxidized ferredoxin
-
Methanothermobacter thermautotrophicus S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin
-
r
1.2.7.8 (indol-3-yl)pyruvate + CoA + oxidized ferredoxin biosynthesis of amino acids from fatty acids Methanothermobacter thermautotrophicus S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin
-
r
1.2.7.8 phenylpyruvate + CoA + oxidized ferredoxin
-
Methanothermobacter thermautotrophicus phenylacetyl-CoA + CO2 + reduced ferredoxin
-
r

Subunits

EC Number Subunits Comment Organism
1.2.7.7 ? x * 55000 + x * 37000 + x * 15000, SDS-PAGE Methanothermobacter thermautotrophicus
1.2.7.7 ? comparison of N-terminal amino acid sequences Methanothermobacter thermautotrophicus
1.2.7.7 ? comparison of N-terminal amino acid sequences Pyrococcus furiosus
1.2.7.7 ? comparison of N-terminal amino acid sequences Thermococcus litoralis
1.2.7.8 tetramer 2 * 67000 + 2 * 23000, alpha2,beta2, SDS-PAGE Methanothermobacter thermautotrophicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.7.7 75
-
at pH 8.0 Methanothermobacter thermautotrophicus
1.2.7.8 80
-
-
Methanothermobacter thermautotrophicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.7.7 9.7
-
at 65°C Methanothermobacter thermautotrophicus
1.2.7.8 10
-
-
Methanothermobacter thermautotrophicus