EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.2.7.7 | thiamine diphosphate | enzyme contains 0.17 mol per 230000 g of protein | Thermococcus litoralis | |
1.2.7.7 | thiamine diphosphate | Km 0.076 mM | Pyrococcus sp. | |
1.2.7.7 | thiamine diphosphate | Km 0.3 mM | Thermococcus litoralis | |
1.2.7.7 | thiamine diphosphate | thiamine diphosphate can be replaced by thiamine or thiamine monophosphate | Thermococcus litoralis | |
1.2.7.7 | thiamine diphosphate | requires for maximal activity of enzyme | Pyrococcus sp. | |
1.2.7.7 | thiamine diphosphate | requires for maximal activity of enzyme | Pyrococcus furiosus | |
1.2.7.7 | thiamine diphosphate | requires for maximal activity of enzyme | Thermococcus litoralis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.2.7.7 | EDTA | 80°C, complete loss of activity | Thermococcus litoralis | |
1.2.7.7 | iodoacetate | 23°C, in a concentration-dependent manner | Thermococcus litoralis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.7.7 | 0.0096 | - |
CoA | pH 6.9, 85°C, under argon | Pyrococcus sp. | |
1.2.7.7 | 0.012 | - |
2-keto-3-methylvalerate | pH 6.9, 85°C, under argon | Thermococcus litoralis | |
1.2.7.7 | 0.017 | - |
oxidized ferredoxin | pH 6.9, 85°C, under argon | Thermococcus litoralis | |
1.2.7.7 | 0.026 | - |
2-ketoisocaproate | pH 6.9, 85°C, under argon | Thermococcus litoralis | |
1.2.7.7 | 0.048 | - |
CO2 | pH 7.0, 85°C, under argon | Thermococcus litoralis | |
1.2.7.7 | 0.049 | - |
2-ketocaproate | pH 6.9, 85°C, under argon | Thermococcus litoralis | |
1.2.7.7 | 0.05 | - |
CoA | pH 6.9, 85°C, under argon | Thermococcus litoralis | |
1.2.7.7 | 0.058 | - |
4-methylthio-2-oxobutanoate | pH 6.9, 85°C, under argon | Thermococcus litoralis | |
1.2.7.7 | 0.094 | - |
2-ketoisovalerate | pH 6.9, 85°C, under argon | Thermococcus litoralis | |
1.2.7.7 | 0.11 | - |
2-ketoisovalerate | pH 6.9, 85°C, under argon | Pyrococcus sp. | |
1.2.7.7 | 0.15 | - |
2-keto-3-methylvalerate | pH 6.9, 85°C, under argon | Pyrococcus sp. | |
1.2.7.7 | 0.17 | - |
2-Ketobutyrate | pH 6.9, 85°C, under argon | Pyrococcus sp. | |
1.2.7.7 | 0.176 | - |
2-ketoisocaproate | pH 6.9, 85°C, under argon | Pyrococcus sp. | |
1.2.7.7 | 0.22 | - |
phenylpyruvate | pH 6.9, 85°C, under argon | Thermococcus litoralis | |
1.2.7.7 | 0.25 | - |
isobutyryl-CoA | pH 7.0, 85°C, under argon | Thermococcus litoralis | |
1.2.7.7 | 0.276 | - |
3-methyl-2-oxobutanoate | pH 6.9, 85°C, under argon | Pyrococcus sp. | |
1.2.7.7 | 0.311 | - |
2-ketocaproate | pH 6.9, 85°C, under argon | Pyrococcus sp. | |
1.2.7.7 | 0.38 | - |
2-Ketobutyrate | pH 6.9, 85°C, under argon | Thermococcus litoralis | |
1.2.7.7 | 0.466 | - |
phenylglyoxylate | pH 6.9, 85°C, under argon | Pyrococcus sp. | |
1.2.7.7 | 0.6 | - |
phenylglyoxalate | pH 6.9, 85°C, under argon | Thermococcus litoralis | |
1.2.7.7 | 0.75 | - |
pyruvate | pH 6.9, 85°C, under argon | Thermococcus litoralis | |
1.2.7.7 | 2.8 | - |
pyruvate | pH 6.9, 85°C, under argon | Pyrococcus sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.2.7.7 | Fe | 11 iron atoms per heterotetramer and two types of FeS are evident | Thermococcus litoralis | |
1.2.7.7 | Mg | required for maximal activity of enzyme | Pyrococcus sp. | |
1.2.7.7 | Mg | required for maximal activity of enzyme | Thermococcus litoralis | |
1.2.7.7 | Mg | Km 3.00 mM | Thermococcus litoralis | |
1.2.7.7 | Mg | Km 0.89 mM | Pyrococcus sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.2.7.7 | 13000 | - |
alpha,beta,gamma,delta, 2 * 47000 + 2 * 34000 + 2 * 23000 + 2 * 13000, SDS PAGE | Pyrococcus sp. |
1.2.7.7 | 13000 | - |
alpha,beta,gamma,delta, 2 * 47000 + 2 * 34000 + 2 * 23000 + 2 * 13000, SDS PAGE | Thermococcus litoralis |
1.2.7.7 | 23000 | - |
alpha,beta,gamma,delta, 2 * 47000 + 2 * 34000 + 2 * 23000 + 2 * 13000, SDS PAGE | Pyrococcus sp. |
1.2.7.7 | 23000 | - |
alpha,beta,gamma,delta, 2 * 47000 + 2 * 34000 + 2 * 23000 + 2 * 13000, SDS PAGE | Thermococcus litoralis |
1.2.7.7 | 34000 | - |
alpha,beta,gamma,delta, 2 * 47000 + 2 * 34000 + 2 * 23000 + 2 * 13000, SDS PAGE | Pyrococcus sp. |
1.2.7.7 | 34000 | - |
alpha,beta,gamma,delta, 2 * 47000 + 2 * 34000 + 2 * 23000 + 2 * 13000, SDS PAGE | Thermococcus litoralis |
1.2.7.7 | 47000 | - |
alpha,beta,gamma,delta, 2 * 47000 + 2 * 34000 + 2 * 23000 + 2 * 13000, SDS PAGE | Pyrococcus sp. |
1.2.7.7 | 47000 | - |
alpha,beta,gamma,delta, 2 * 47000 + 2 * 34000 + 2 * 23000 + 2 * 13000, SDS PAGE | Thermococcus litoralis |
1.2.7.7 | 230000 | - |
calculated from amino acid sequence | Thermococcus litoralis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.7.7 | additional information | Thermococcus sp. | - |
? | - |
? | |
1.2.7.7 | additional information | Pyrococcus furiosus | enzyme catalyses the CoA-dependent oxidation of branched-chain 2-ketoacids | ? | - |
? | |
1.2.7.7 | additional information | Thermococcus litoralis | enzyme catalyses the CoA-dependent oxidation of branched-chain 2-ketoacids | ? | - |
? | |
1.2.7.7 | additional information | Thermococcus litoralis | enzyme functions in the biosynthesis of branched-chain amino acids from volatile fatty acids that are excreted under nutrient-sufficient conditions | ? | - |
? | |
1.2.7.7 | additional information | Thermococcus sp. ES-1 | - |
? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.7.7 | Pyrococcus furiosus | - |
DSM 3638 | - |
1.2.7.7 | Pyrococcus sp. | - |
ES-4 | - |
1.2.7.7 | Pyrococcus sp. ES-4 | - |
ES-4 | - |
1.2.7.7 | Thermococcus litoralis | - |
- |
- |
1.2.7.7 | Thermococcus sp. | - |
ES-1 | - |
1.2.7.7 | Thermococcus sp. ES-1 | - |
ES-1 | - |
EC Number | Oxidation Stability | Organism |
---|---|---|
1.2.7.7 | about 50% of the enzyme activity in the cell extract is lost after exposure to air for 2 min, no activity loss if the extract is under argon | Thermococcus litoralis |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.7.7 | under anaerobic conditions | Pyrococcus sp. |
1.2.7.7 | under anaerobic conditions | Pyrococcus furiosus |
1.2.7.7 | under anaerobic conditions | Thermococcus sp. |
1.2.7.7 | under anaerobic conditions | Thermococcus litoralis |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.2.7.7 | 3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin = S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | this enzyme is one of four 2-oxoacid oxidoreductases: indolepyruvate oxidoreductase, EC 1.2.7.8, pyruvate oxidoreductase, EC 1.2.7.1., 2-oxogluterate oxidoreductase, EC 1.2.7.3 | Pyrococcus sp. | |
1.2.7.7 | 3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin = S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | this enzyme is one of four 2-oxoacid oxidoreductases: indolepyruvate oxidoreductase, EC 1.2.7.8, pyruvate oxidoreductase, EC 1.2.7.1., 2-oxogluterate oxidoreductase, EC 1.2.7.3 | Pyrococcus furiosus | |
1.2.7.7 | 3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin = S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | this enzyme is one of four 2-oxoacid oxidoreductases: indolepyruvate oxidoreductase, EC 1.2.7.8, pyruvate oxidoreductase, EC 1.2.7.1., 2-oxogluterate oxidoreductase, EC 1.2.7.3 | Thermococcus sp. | |
1.2.7.7 | 3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin = S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | this enzyme is one of four 2-oxoacid oxidoreductases: indolepyruvate oxidoreductase, EC 1.2.7.8, pyruvate oxidoreductase, EC 1.2.7.1., 2-oxogluterate oxidoreductase, EC 1.2.7.3 | Thermococcus litoralis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.2.7.7 | 1 | - |
Kommentar | Thermococcus litoralis |
1.2.7.7 | 46 | - |
- |
Thermococcus litoralis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.7.7 | 2-keto-3-methylvalerate + CoA + oxidized ferredoxin | - |
Pyrococcus sp. | 2-methylbutanoyl-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | 2-keto-3-methylvalerate + CoA + oxidized ferredoxin | - |
Pyrococcus furiosus | 2-methylbutanoyl-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | 2-keto-3-methylvalerate + CoA + oxidized ferredoxin | - |
Thermococcus sp. | 2-methylbutanoyl-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | 2-keto-3-methylvalerate + CoA + oxidized ferredoxin | - |
Thermococcus litoralis | 2-methylbutanoyl-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | 2-keto-3-methylvalerate + CoA + oxidized ferredoxin | - |
Pyrococcus sp. ES-4 | 2-methylbutanoyl-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | 2-ketobutyrate + CoA + oxidized ferredoxin | - |
Pyrococcus sp. | propanoyl-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | 2-ketobutyrate + CoA + oxidized ferredoxin | - |
Pyrococcus furiosus | propanoyl-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | 2-ketobutyrate + CoA + oxidized ferredoxin | - |
Thermococcus sp. | propanoyl-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | 2-ketobutyrate + CoA + oxidized ferredoxin | - |
Thermococcus litoralis | propanoyl-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | 2-ketocaproate + CoA + oxidized ferredoxin | - |
Pyrococcus sp. | pentanoyl-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | 2-ketocaproate + CoA + oxidized ferredoxin | - |
Pyrococcus furiosus | pentanoyl-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | 2-ketocaproate + CoA + oxidized ferredoxin | - |
Thermococcus sp. | pentanoyl-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | 2-ketocaproate + CoA + oxidized ferredoxin | - |
Thermococcus litoralis | pentanoyl-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | 2-ketoisocaproate + CoA + oxidized ferredoxin | - |
Pyrococcus sp. | S-(3-methylbutanoyl)-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | 2-ketoisocaproate + CoA + oxidized ferredoxin | - |
Pyrococcus furiosus | S-(3-methylbutanoyl)-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | 2-ketoisocaproate + CoA + oxidized ferredoxin | - |
Thermococcus sp. | S-(3-methylbutanoyl)-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | 2-ketoisocaproate + CoA + oxidized ferredoxin | - |
Thermococcus litoralis | S-(3-methylbutanoyl)-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | 2-ketoisovalerate + CoA + oxidized ferredoxin | - |
Pyrococcus sp. | S-(2-methylpropanoyl)-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | 2-ketoisovalerate + CoA + oxidized ferredoxin | - |
Pyrococcus furiosus | S-(2-methylpropanoyl)-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | 2-ketoisovalerate + CoA + oxidized ferredoxin | - |
Thermococcus sp. | S-(2-methylpropanoyl)-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | 2-ketoisovalerate + CoA + oxidized ferredoxin | or benzyl viologen or methyl viologen as electron acceptor | Thermococcus litoralis | S-(2-methylpropanoyl)-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | 2-ketoisovalerate + CoA + oxidized ferredoxin | - |
Pyrococcus sp. ES-4 | S-(2-methylpropanoyl)-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | 2-ketoisovalerate + CoA + oxidized ferredoxin | - |
Thermococcus sp. ES-1 | S-(2-methylpropanoyl)-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | 3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin | - |
Pyrococcus sp. | S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + H+ | - |
r | |
1.2.7.7 | 3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin | - |
Thermococcus litoralis | S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + H+ | - |
r | |
1.2.7.7 | 4-methylthio-2-oxobutanoate + CoA + oxidized ferredoxin | - |
Pyrococcus sp. | 3-methylthio-2-oxopropanoyl-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | 4-methylthio-2-oxobutanoate + CoA + oxidized ferredoxin | - |
Pyrococcus furiosus | 3-methylthio-2-oxopropanoyl-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | 4-methylthio-2-oxobutanoate + CoA + oxidized ferredoxin | - |
Thermococcus sp. | 3-methylthio-2-oxopropanoyl-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | 4-methylthio-2-oxobutanoate + CoA + oxidized ferredoxin | - |
Thermococcus litoralis | 3-methylthio-2-oxopropanoyl-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | glyoxylate + CoA + oxidized ferredoxin | - |
Pyrococcus sp. | formyl-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | glyoxylate + CoA + oxidized ferredoxin | - |
Pyrococcus furiosus | formyl-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | glyoxylate + CoA + oxidized ferredoxin | - |
Thermococcus sp. | formyl-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | glyoxylate + CoA + oxidized ferredoxin | - |
Thermococcus litoralis | formyl-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | hydroxyphenylpyruvate + CoA + oxidized ferredoxin | - |
Pyrococcus sp. | 4-hydroxyphenylacetyl-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | hydroxyphenylpyruvate + CoA + oxidized ferredoxin | - |
Pyrococcus furiosus | 4-hydroxyphenylacetyl-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | hydroxyphenylpyruvate + CoA + oxidized ferredoxin | - |
Thermococcus sp. | 4-hydroxyphenylacetyl-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | hydroxyphenylpyruvate + CoA + oxidized ferredoxin | - |
Thermococcus litoralis | 4-hydroxyphenylacetyl-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | indolpyruvate + CoA + oxidized ferredoxin | - |
Pyrococcus sp. | indolacetyl-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | indolpyruvate + CoA + oxidized ferredoxin | - |
Pyrococcus furiosus | indolacetyl-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | indolpyruvate + CoA + oxidized ferredoxin | - |
Thermococcus sp. | indolacetyl-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | indolpyruvate + CoA + oxidized ferredoxin | - |
Thermococcus litoralis | indolacetyl-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | isobutyryl-CoA + CO2 + reduced benzyl viologen | - |
Pyrococcus sp. | 2-oxoisopentanoate + CoA + benzyl viologen | - |
? | |
1.2.7.7 | isobutyryl-CoA + CO2 + reduced benzyl viologen | - |
Pyrococcus furiosus | 2-oxoisopentanoate + CoA + benzyl viologen | - |
? | |
1.2.7.7 | isobutyryl-CoA + CO2 + reduced benzyl viologen | - |
Thermococcus sp. | 2-oxoisopentanoate + CoA + benzyl viologen | - |
? | |
1.2.7.7 | isobutyryl-CoA + CO2 + reduced benzyl viologen | - |
Thermococcus litoralis | 2-oxoisopentanoate + CoA + benzyl viologen | - |
? | |
1.2.7.7 | additional information | - |
Thermococcus sp. | ? | - |
? | |
1.2.7.7 | additional information | enzyme catalyses the CoA-dependent oxidation of branched-chain 2-ketoacids | Pyrococcus sp. | ? | - |
? | |
1.2.7.7 | additional information | enzyme catalyses the CoA-dependent oxidation of branched-chain 2-ketoacids | Pyrococcus furiosus | ? | - |
? | |
1.2.7.7 | additional information | enzyme catalyses the CoA-dependent oxidation of branched-chain 2-ketoacids | Thermococcus sp. | ? | - |
? | |
1.2.7.7 | additional information | enzyme catalyses the CoA-dependent oxidation of branched-chain 2-ketoacids | Thermococcus litoralis | ? | - |
? | |
1.2.7.7 | additional information | no substrate are 2-ketoglutarate, 2-ketomalonate, imidazolpyruvate | Pyrococcus sp. | ? | - |
? | |
1.2.7.7 | additional information | no substrate are 2-ketoglutarate, 2-ketomalonate, imidazolpyruvate | Pyrococcus furiosus | ? | - |
? | |
1.2.7.7 | additional information | no substrate are 2-ketoglutarate, 2-ketomalonate, imidazolpyruvate | Thermococcus sp. | ? | - |
? | |
1.2.7.7 | additional information | no substrate are 2-ketoglutarate, 2-ketomalonate, imidazolpyruvate | Thermococcus litoralis | ? | - |
? | |
1.2.7.7 | additional information | enzyme functions in the biosynthesis of branched-chain amino acids from volatile fatty acids that are excreted under nutrient-sufficient conditions | Thermococcus litoralis | ? | - |
? | |
1.2.7.7 | additional information | enzyme catalyses the CoA-dependent oxidation of branched-chain 2-ketoacids | Pyrococcus sp. ES-4 | ? | - |
? | |
1.2.7.7 | additional information | no substrate are 2-ketoglutarate, 2-ketomalonate, imidazolpyruvate | Pyrococcus sp. ES-4 | ? | - |
? | |
1.2.7.7 | additional information | - |
Thermococcus sp. ES-1 | ? | - |
? | |
1.2.7.7 | additional information | enzyme catalyses the CoA-dependent oxidation of branched-chain 2-ketoacids | Thermococcus sp. ES-1 | ? | - |
? | |
1.2.7.7 | additional information | no substrate are 2-ketoglutarate, 2-ketomalonate, imidazolpyruvate | Thermococcus sp. ES-1 | ? | - |
? | |
1.2.7.7 | phenylglyoxylate + CoA + oxidized ferredoxin | - |
Pyrococcus sp. | benzoyl-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | phenylglyoxylate + CoA + oxidized ferredoxin | - |
Pyrococcus furiosus | benzoyl-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | phenylglyoxylate + CoA + oxidized ferredoxin | - |
Thermococcus sp. | benzoyl-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | phenylglyoxylate + CoA + oxidized ferredoxin | - |
Thermococcus litoralis | benzoyl-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | phenylpyruvate + CoA + oxidized ferredoxin | - |
Pyrococcus sp. | phenylacetyl-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | phenylpyruvate + CoA + oxidized ferredoxin | - |
Pyrococcus furiosus | phenylacetyl-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | phenylpyruvate + CoA + oxidized ferredoxin | - |
Thermococcus sp. | phenylacetyl-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | phenylpyruvate + CoA + oxidized ferredoxin | - |
Thermococcus litoralis | phenylacetyl-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | pyruvate + CoA + oxidized ferredoxin | - |
Pyrococcus sp. | acetyl-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | pyruvate + CoA + oxidized ferredoxin | - |
Pyrococcus furiosus | acetyl-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | pyruvate + CoA + oxidized ferredoxin | - |
Thermococcus sp. | acetyl-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | pyruvate + CoA + oxidized ferredoxin | - |
Thermococcus litoralis | acetyl-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | pyruvate + CoA + oxidized ferredoxin | - |
Pyrococcus sp. ES-4 | acetyl-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | pyruvate + CoA + oxidized ferredoxin | - |
Thermococcus sp. ES-1 | acetyl-CoA + CO2 + reduced ferredoxin | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.2.7.7 | octamer | alpha,beta,gamma,delta, 2 * 47000 + 2 * 34000 + 2 * 23000 + 2 * 13000, SDS PAGE | Pyrococcus sp. |
1.2.7.7 | octamer | alpha,beta,gamma,delta, 2 * 47000 + 2 * 34000 + 2 * 23000 + 2 * 13000, SDS PAGE | Thermococcus litoralis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.2.7.7 | 90 | - |
ketoisovalerate oxidation | Thermococcus litoralis |
1.2.7.7 | 98 | - |
ketoisovalerate oxidation | Pyrococcus sp. |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.2.7.7 | 20 | - |
enzyme is inactive at 20°C | Pyrococcus sp. |
1.2.7.7 | 20 | - |
enzyme is inactive at 20°C | Thermococcus litoralis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.2.7.7 | 85 | - |
pH 8.0, Tris-HCl, half-life 3 h | Thermococcus litoralis |
1.2.7.7 | 95 | - |
pH 8.0, Tris-HCl, half-life 50 min | Thermococcus litoralis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.7.7 | 0.0133 | - |
glyoxylate | pH 6.9, 85°C, under argon | Thermococcus litoralis | |
1.2.7.7 | 0.267 | - |
pyruvate | pH 6.9, 85°C, under argon | Thermococcus litoralis | |
1.2.7.7 | 0.433 | - |
indolpyruvate | pH 6.9, 85°C, under argon | Thermococcus litoralis | |
1.2.7.7 | 0.467 | - |
phenylpyruvate | pH 6.9, 85°C, under argon | Pyrococcus sp. | |
1.2.7.7 | 0.6 | - |
phenylpyruvate | pH 6.9, 85°C, under argon | Thermococcus litoralis | |
1.2.7.7 | 0.667 | - |
4-methylthio-2-oxobutanoate | pH 6.9, 85°C, under argon | Thermococcus litoralis | |
1.2.7.7 | 0.833 | - |
hydroxyphenylpyruvate | pH 6.9, 85°C, under argon | Thermococcus litoralis | |
1.2.7.7 | 0.833 | - |
phenylglyoxalate | pH 6.9, 85°C, under argon | Thermococcus litoralis | |
1.2.7.7 | 0.933 | - |
2-ketocaproate | pH 6.9, 85°C, under argon | Pyrococcus sp. | |
1.2.7.7 | 1 | - |
2-ketocaproate | pH 6.9, 85°C, under argon | Thermococcus litoralis | |
1.2.7.7 | 1.07 | - |
3-methyl-2-oxobutanoate | pH 6.9, 85°C, under argon | Pyrococcus sp. | |
1.2.7.7 | 1.27 | - |
2-keto-3-methylvalerate | pH 6.9, 85°C, under argon | Thermococcus litoralis | |
1.2.7.7 | 1.33 | - |
phenylglyoxylate | pH 6.9, 85°C, under argon | Pyrococcus sp. | |
1.2.7.7 | 1.47 | - |
2-Ketobutyrate | pH 6.9, 85°C, under argon | Pyrococcus sp. | |
1.2.7.7 | 1.53 | - |
oxidized ferredoxin | pH 6.9, 85°C, under argon | Thermococcus litoralis | |
1.2.7.7 | 1.73 | - |
pyruvate | pH 6.9, 85°C, under argon | Pyrococcus sp. | |
1.2.7.7 | 2 | - |
2-ketoisocaproate | pH 6.9, 85°C, under argon | Thermococcus litoralis | |
1.2.7.7 | 2.07 | - |
2-keto-3-methylvalerate | pH 6.9, 85°C, under argon | Pyrococcus sp. | |
1.2.7.7 | 2.47 | - |
2-ketoisocaproate | pH 6.9, 85°C, under argon | Pyrococcus sp. | |
1.2.7.7 | 3 | - |
2-ketoisovalerate | pH 6.9, 85°C, under argon | Thermococcus litoralis | |
1.2.7.7 | 3.67 | - |
2-ketoisovalerate | pH 6.9, 85°C, under argon | Pyrococcus sp. | |
1.2.7.7 | 5 | - |
CoA | pH 6.9, 85°C, under argon | Thermococcus litoralis | |
1.2.7.7 | 5.6 | - |
CoA | pH 6.9, 85°C, under argon | Pyrococcus sp. | |
1.2.7.7 | 22.3 | - |
2-Ketobutyrate | pH 6.9, 85°C, under argon | Thermococcus litoralis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.2.7.7 | 7 | - |
- |
Thermococcus litoralis |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.2.7.7 | 6 | 8 | pH 6.0: less than 50% loss of activity, pH 8.0: 25% loss of activity | Thermococcus litoralis |