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Literature summary extracted from

  • Heider, J.; Mai, X.; Adams, M.W.
    Characterization of 2-ketoisovalerate ferredoxin oxidoreductase, a new and reversible coenzyme A-dependent enzyme involved in peptide fermentation by hyperthermophilic archaea (1996), J. Bacteriol., 178, 780-787.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.7.7 thiamine diphosphate enzyme contains 0.17 mol per 230000 g of protein Thermococcus litoralis
1.2.7.7 thiamine diphosphate Km 0.076 mM Pyrococcus sp.
1.2.7.7 thiamine diphosphate Km 0.3 mM Thermococcus litoralis
1.2.7.7 thiamine diphosphate thiamine diphosphate can be replaced by thiamine or thiamine monophosphate Thermococcus litoralis
1.2.7.7 thiamine diphosphate requires for maximal activity of enzyme Pyrococcus sp.
1.2.7.7 thiamine diphosphate requires for maximal activity of enzyme Pyrococcus furiosus
1.2.7.7 thiamine diphosphate requires for maximal activity of enzyme Thermococcus litoralis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.7.7 EDTA 80°C, complete loss of activity Thermococcus litoralis
1.2.7.7 iodoacetate 23°C, in a concentration-dependent manner Thermococcus litoralis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.7.7 0.0096
-
CoA pH 6.9, 85°C, under argon Pyrococcus sp.
1.2.7.7 0.012
-
2-keto-3-methylvalerate pH 6.9, 85°C, under argon Thermococcus litoralis
1.2.7.7 0.017
-
oxidized ferredoxin pH 6.9, 85°C, under argon Thermococcus litoralis
1.2.7.7 0.026
-
2-ketoisocaproate pH 6.9, 85°C, under argon Thermococcus litoralis
1.2.7.7 0.048
-
CO2 pH 7.0, 85°C, under argon Thermococcus litoralis
1.2.7.7 0.049
-
2-ketocaproate pH 6.9, 85°C, under argon Thermococcus litoralis
1.2.7.7 0.05
-
CoA pH 6.9, 85°C, under argon Thermococcus litoralis
1.2.7.7 0.058
-
4-methylthio-2-oxobutanoate pH 6.9, 85°C, under argon Thermococcus litoralis
1.2.7.7 0.094
-
2-ketoisovalerate pH 6.9, 85°C, under argon Thermococcus litoralis
1.2.7.7 0.11
-
2-ketoisovalerate pH 6.9, 85°C, under argon Pyrococcus sp.
1.2.7.7 0.15
-
2-keto-3-methylvalerate pH 6.9, 85°C, under argon Pyrococcus sp.
1.2.7.7 0.17
-
2-Ketobutyrate pH 6.9, 85°C, under argon Pyrococcus sp.
1.2.7.7 0.176
-
2-ketoisocaproate pH 6.9, 85°C, under argon Pyrococcus sp.
1.2.7.7 0.22
-
phenylpyruvate pH 6.9, 85°C, under argon Thermococcus litoralis
1.2.7.7 0.25
-
isobutyryl-CoA pH 7.0, 85°C, under argon Thermococcus litoralis
1.2.7.7 0.276
-
3-methyl-2-oxobutanoate pH 6.9, 85°C, under argon Pyrococcus sp.
1.2.7.7 0.311
-
2-ketocaproate pH 6.9, 85°C, under argon Pyrococcus sp.
1.2.7.7 0.38
-
2-Ketobutyrate pH 6.9, 85°C, under argon Thermococcus litoralis
1.2.7.7 0.466
-
phenylglyoxylate pH 6.9, 85°C, under argon Pyrococcus sp.
1.2.7.7 0.6
-
phenylglyoxalate pH 6.9, 85°C, under argon Thermococcus litoralis
1.2.7.7 0.75
-
pyruvate pH 6.9, 85°C, under argon Thermococcus litoralis
1.2.7.7 2.8
-
pyruvate pH 6.9, 85°C, under argon Pyrococcus sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.7.7 Fe 11 iron atoms per heterotetramer and two types of FeS are evident Thermococcus litoralis
1.2.7.7 Mg required for maximal activity of enzyme Pyrococcus sp.
1.2.7.7 Mg required for maximal activity of enzyme Thermococcus litoralis
1.2.7.7 Mg Km 3.00 mM Thermococcus litoralis
1.2.7.7 Mg Km 0.89 mM Pyrococcus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.7.7 13000
-
alpha,beta,gamma,delta, 2 * 47000 + 2 * 34000 + 2 * 23000 + 2 * 13000, SDS PAGE Pyrococcus sp.
1.2.7.7 13000
-
alpha,beta,gamma,delta, 2 * 47000 + 2 * 34000 + 2 * 23000 + 2 * 13000, SDS PAGE Thermococcus litoralis
1.2.7.7 23000
-
alpha,beta,gamma,delta, 2 * 47000 + 2 * 34000 + 2 * 23000 + 2 * 13000, SDS PAGE Pyrococcus sp.
1.2.7.7 23000
-
alpha,beta,gamma,delta, 2 * 47000 + 2 * 34000 + 2 * 23000 + 2 * 13000, SDS PAGE Thermococcus litoralis
1.2.7.7 34000
-
alpha,beta,gamma,delta, 2 * 47000 + 2 * 34000 + 2 * 23000 + 2 * 13000, SDS PAGE Pyrococcus sp.
1.2.7.7 34000
-
alpha,beta,gamma,delta, 2 * 47000 + 2 * 34000 + 2 * 23000 + 2 * 13000, SDS PAGE Thermococcus litoralis
1.2.7.7 47000
-
alpha,beta,gamma,delta, 2 * 47000 + 2 * 34000 + 2 * 23000 + 2 * 13000, SDS PAGE Pyrococcus sp.
1.2.7.7 47000
-
alpha,beta,gamma,delta, 2 * 47000 + 2 * 34000 + 2 * 23000 + 2 * 13000, SDS PAGE Thermococcus litoralis
1.2.7.7 230000
-
calculated from amino acid sequence Thermococcus litoralis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.7.7 additional information Thermococcus sp.
-
?
-
?
1.2.7.7 additional information Pyrococcus furiosus enzyme catalyses the CoA-dependent oxidation of branched-chain 2-ketoacids ?
-
?
1.2.7.7 additional information Thermococcus litoralis enzyme catalyses the CoA-dependent oxidation of branched-chain 2-ketoacids ?
-
?
1.2.7.7 additional information Thermococcus litoralis enzyme functions in the biosynthesis of branched-chain amino acids from volatile fatty acids that are excreted under nutrient-sufficient conditions ?
-
?
1.2.7.7 additional information Thermococcus sp. ES-1
-
?
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.7 Pyrococcus furiosus
-
DSM 3638
-
1.2.7.7 Pyrococcus sp.
-
ES-4
-
1.2.7.7 Pyrococcus sp. ES-4
-
ES-4
-
1.2.7.7 Thermococcus litoralis
-
-
-
1.2.7.7 Thermococcus sp.
-
ES-1
-
1.2.7.7 Thermococcus sp. ES-1
-
ES-1
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.2.7.7 about 50% of the enzyme activity in the cell extract is lost after exposure to air for 2 min, no activity loss if the extract is under argon Thermococcus litoralis

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.7.7 under anaerobic conditions Pyrococcus sp.
1.2.7.7 under anaerobic conditions Pyrococcus furiosus
1.2.7.7 under anaerobic conditions Thermococcus sp.
1.2.7.7 under anaerobic conditions Thermococcus litoralis

Reaction

EC Number Reaction Comment Organism Reaction ID
1.2.7.7 3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin = S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + 2 H+ this enzyme is one of four 2-oxoacid oxidoreductases: indolepyruvate oxidoreductase, EC 1.2.7.8, pyruvate oxidoreductase, EC 1.2.7.1., 2-oxogluterate oxidoreductase, EC 1.2.7.3 Pyrococcus sp.
1.2.7.7 3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin = S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + 2 H+ this enzyme is one of four 2-oxoacid oxidoreductases: indolepyruvate oxidoreductase, EC 1.2.7.8, pyruvate oxidoreductase, EC 1.2.7.1., 2-oxogluterate oxidoreductase, EC 1.2.7.3 Pyrococcus furiosus
1.2.7.7 3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin = S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + 2 H+ this enzyme is one of four 2-oxoacid oxidoreductases: indolepyruvate oxidoreductase, EC 1.2.7.8, pyruvate oxidoreductase, EC 1.2.7.1., 2-oxogluterate oxidoreductase, EC 1.2.7.3 Thermococcus sp.
1.2.7.7 3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin = S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + 2 H+ this enzyme is one of four 2-oxoacid oxidoreductases: indolepyruvate oxidoreductase, EC 1.2.7.8, pyruvate oxidoreductase, EC 1.2.7.1., 2-oxogluterate oxidoreductase, EC 1.2.7.3 Thermococcus litoralis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.7.7 1
-
Kommentar Thermococcus litoralis
1.2.7.7 46
-
-
Thermococcus litoralis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.7 2-keto-3-methylvalerate + CoA + oxidized ferredoxin
-
Pyrococcus sp. 2-methylbutanoyl-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 2-keto-3-methylvalerate + CoA + oxidized ferredoxin
-
Pyrococcus furiosus 2-methylbutanoyl-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 2-keto-3-methylvalerate + CoA + oxidized ferredoxin
-
Thermococcus sp. 2-methylbutanoyl-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 2-keto-3-methylvalerate + CoA + oxidized ferredoxin
-
Thermococcus litoralis 2-methylbutanoyl-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 2-keto-3-methylvalerate + CoA + oxidized ferredoxin
-
Pyrococcus sp. ES-4 2-methylbutanoyl-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 2-ketobutyrate + CoA + oxidized ferredoxin
-
Pyrococcus sp. propanoyl-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 2-ketobutyrate + CoA + oxidized ferredoxin
-
Pyrococcus furiosus propanoyl-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 2-ketobutyrate + CoA + oxidized ferredoxin
-
Thermococcus sp. propanoyl-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 2-ketobutyrate + CoA + oxidized ferredoxin
-
Thermococcus litoralis propanoyl-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 2-ketocaproate + CoA + oxidized ferredoxin
-
Pyrococcus sp. pentanoyl-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 2-ketocaproate + CoA + oxidized ferredoxin
-
Pyrococcus furiosus pentanoyl-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 2-ketocaproate + CoA + oxidized ferredoxin
-
Thermococcus sp. pentanoyl-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 2-ketocaproate + CoA + oxidized ferredoxin
-
Thermococcus litoralis pentanoyl-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 2-ketoisocaproate + CoA + oxidized ferredoxin
-
Pyrococcus sp. S-(3-methylbutanoyl)-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 2-ketoisocaproate + CoA + oxidized ferredoxin
-
Pyrococcus furiosus S-(3-methylbutanoyl)-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 2-ketoisocaproate + CoA + oxidized ferredoxin
-
Thermococcus sp. S-(3-methylbutanoyl)-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 2-ketoisocaproate + CoA + oxidized ferredoxin
-
Thermococcus litoralis S-(3-methylbutanoyl)-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 2-ketoisovalerate + CoA + oxidized ferredoxin
-
Pyrococcus sp. S-(2-methylpropanoyl)-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 2-ketoisovalerate + CoA + oxidized ferredoxin
-
Pyrococcus furiosus S-(2-methylpropanoyl)-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 2-ketoisovalerate + CoA + oxidized ferredoxin
-
Thermococcus sp. S-(2-methylpropanoyl)-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 2-ketoisovalerate + CoA + oxidized ferredoxin or benzyl viologen or methyl viologen as electron acceptor Thermococcus litoralis S-(2-methylpropanoyl)-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 2-ketoisovalerate + CoA + oxidized ferredoxin
-
Pyrococcus sp. ES-4 S-(2-methylpropanoyl)-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 2-ketoisovalerate + CoA + oxidized ferredoxin
-
Thermococcus sp. ES-1 S-(2-methylpropanoyl)-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin
-
Pyrococcus sp. S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + H+
-
r
1.2.7.7 3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin
-
Thermococcus litoralis S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + H+
-
r
1.2.7.7 4-methylthio-2-oxobutanoate + CoA + oxidized ferredoxin
-
Pyrococcus sp. 3-methylthio-2-oxopropanoyl-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 4-methylthio-2-oxobutanoate + CoA + oxidized ferredoxin
-
Pyrococcus furiosus 3-methylthio-2-oxopropanoyl-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 4-methylthio-2-oxobutanoate + CoA + oxidized ferredoxin
-
Thermococcus sp. 3-methylthio-2-oxopropanoyl-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 4-methylthio-2-oxobutanoate + CoA + oxidized ferredoxin
-
Thermococcus litoralis 3-methylthio-2-oxopropanoyl-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 glyoxylate + CoA + oxidized ferredoxin
-
Pyrococcus sp. formyl-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 glyoxylate + CoA + oxidized ferredoxin
-
Pyrococcus furiosus formyl-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 glyoxylate + CoA + oxidized ferredoxin
-
Thermococcus sp. formyl-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 glyoxylate + CoA + oxidized ferredoxin
-
Thermococcus litoralis formyl-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 hydroxyphenylpyruvate + CoA + oxidized ferredoxin
-
Pyrococcus sp. 4-hydroxyphenylacetyl-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 hydroxyphenylpyruvate + CoA + oxidized ferredoxin
-
Pyrococcus furiosus 4-hydroxyphenylacetyl-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 hydroxyphenylpyruvate + CoA + oxidized ferredoxin
-
Thermococcus sp. 4-hydroxyphenylacetyl-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 hydroxyphenylpyruvate + CoA + oxidized ferredoxin
-
Thermococcus litoralis 4-hydroxyphenylacetyl-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 indolpyruvate + CoA + oxidized ferredoxin
-
Pyrococcus sp. indolacetyl-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 indolpyruvate + CoA + oxidized ferredoxin
-
Pyrococcus furiosus indolacetyl-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 indolpyruvate + CoA + oxidized ferredoxin
-
Thermococcus sp. indolacetyl-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 indolpyruvate + CoA + oxidized ferredoxin
-
Thermococcus litoralis indolacetyl-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 isobutyryl-CoA + CO2 + reduced benzyl viologen
-
Pyrococcus sp. 2-oxoisopentanoate + CoA + benzyl viologen
-
?
1.2.7.7 isobutyryl-CoA + CO2 + reduced benzyl viologen
-
Pyrococcus furiosus 2-oxoisopentanoate + CoA + benzyl viologen
-
?
1.2.7.7 isobutyryl-CoA + CO2 + reduced benzyl viologen
-
Thermococcus sp. 2-oxoisopentanoate + CoA + benzyl viologen
-
?
1.2.7.7 isobutyryl-CoA + CO2 + reduced benzyl viologen
-
Thermococcus litoralis 2-oxoisopentanoate + CoA + benzyl viologen
-
?
1.2.7.7 additional information
-
Thermococcus sp. ?
-
?
1.2.7.7 additional information enzyme catalyses the CoA-dependent oxidation of branched-chain 2-ketoacids Pyrococcus sp. ?
-
?
1.2.7.7 additional information enzyme catalyses the CoA-dependent oxidation of branched-chain 2-ketoacids Pyrococcus furiosus ?
-
?
1.2.7.7 additional information enzyme catalyses the CoA-dependent oxidation of branched-chain 2-ketoacids Thermococcus sp. ?
-
?
1.2.7.7 additional information enzyme catalyses the CoA-dependent oxidation of branched-chain 2-ketoacids Thermococcus litoralis ?
-
?
1.2.7.7 additional information no substrate are 2-ketoglutarate, 2-ketomalonate, imidazolpyruvate Pyrococcus sp. ?
-
?
1.2.7.7 additional information no substrate are 2-ketoglutarate, 2-ketomalonate, imidazolpyruvate Pyrococcus furiosus ?
-
?
1.2.7.7 additional information no substrate are 2-ketoglutarate, 2-ketomalonate, imidazolpyruvate Thermococcus sp. ?
-
?
1.2.7.7 additional information no substrate are 2-ketoglutarate, 2-ketomalonate, imidazolpyruvate Thermococcus litoralis ?
-
?
1.2.7.7 additional information enzyme functions in the biosynthesis of branched-chain amino acids from volatile fatty acids that are excreted under nutrient-sufficient conditions Thermococcus litoralis ?
-
?
1.2.7.7 additional information enzyme catalyses the CoA-dependent oxidation of branched-chain 2-ketoacids Pyrococcus sp. ES-4 ?
-
?
1.2.7.7 additional information no substrate are 2-ketoglutarate, 2-ketomalonate, imidazolpyruvate Pyrococcus sp. ES-4 ?
-
?
1.2.7.7 additional information
-
Thermococcus sp. ES-1 ?
-
?
1.2.7.7 additional information enzyme catalyses the CoA-dependent oxidation of branched-chain 2-ketoacids Thermococcus sp. ES-1 ?
-
?
1.2.7.7 additional information no substrate are 2-ketoglutarate, 2-ketomalonate, imidazolpyruvate Thermococcus sp. ES-1 ?
-
?
1.2.7.7 phenylglyoxylate + CoA + oxidized ferredoxin
-
Pyrococcus sp. benzoyl-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 phenylglyoxylate + CoA + oxidized ferredoxin
-
Pyrococcus furiosus benzoyl-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 phenylglyoxylate + CoA + oxidized ferredoxin
-
Thermococcus sp. benzoyl-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 phenylglyoxylate + CoA + oxidized ferredoxin
-
Thermococcus litoralis benzoyl-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 phenylpyruvate + CoA + oxidized ferredoxin
-
Pyrococcus sp. phenylacetyl-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 phenylpyruvate + CoA + oxidized ferredoxin
-
Pyrococcus furiosus phenylacetyl-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 phenylpyruvate + CoA + oxidized ferredoxin
-
Thermococcus sp. phenylacetyl-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 phenylpyruvate + CoA + oxidized ferredoxin
-
Thermococcus litoralis phenylacetyl-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 pyruvate + CoA + oxidized ferredoxin
-
Pyrococcus sp. acetyl-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 pyruvate + CoA + oxidized ferredoxin
-
Pyrococcus furiosus acetyl-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 pyruvate + CoA + oxidized ferredoxin
-
Thermococcus sp. acetyl-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 pyruvate + CoA + oxidized ferredoxin
-
Thermococcus litoralis acetyl-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 pyruvate + CoA + oxidized ferredoxin
-
Pyrococcus sp. ES-4 acetyl-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 pyruvate + CoA + oxidized ferredoxin
-
Thermococcus sp. ES-1 acetyl-CoA + CO2 + reduced ferredoxin
-
?

Subunits

EC Number Subunits Comment Organism
1.2.7.7 octamer alpha,beta,gamma,delta, 2 * 47000 + 2 * 34000 + 2 * 23000 + 2 * 13000, SDS PAGE Pyrococcus sp.
1.2.7.7 octamer alpha,beta,gamma,delta, 2 * 47000 + 2 * 34000 + 2 * 23000 + 2 * 13000, SDS PAGE Thermococcus litoralis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.7.7 90
-
ketoisovalerate oxidation Thermococcus litoralis
1.2.7.7 98
-
ketoisovalerate oxidation Pyrococcus sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.2.7.7 20
-
enzyme is inactive at 20°C Pyrococcus sp.
1.2.7.7 20
-
enzyme is inactive at 20°C Thermococcus litoralis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.7.7 85
-
pH 8.0, Tris-HCl, half-life 3 h Thermococcus litoralis
1.2.7.7 95
-
pH 8.0, Tris-HCl, half-life 50 min Thermococcus litoralis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.7.7 0.0133
-
glyoxylate pH 6.9, 85°C, under argon Thermococcus litoralis
1.2.7.7 0.267
-
pyruvate pH 6.9, 85°C, under argon Thermococcus litoralis
1.2.7.7 0.433
-
indolpyruvate pH 6.9, 85°C, under argon Thermococcus litoralis
1.2.7.7 0.467
-
phenylpyruvate pH 6.9, 85°C, under argon Pyrococcus sp.
1.2.7.7 0.6
-
phenylpyruvate pH 6.9, 85°C, under argon Thermococcus litoralis
1.2.7.7 0.667
-
4-methylthio-2-oxobutanoate pH 6.9, 85°C, under argon Thermococcus litoralis
1.2.7.7 0.833
-
hydroxyphenylpyruvate pH 6.9, 85°C, under argon Thermococcus litoralis
1.2.7.7 0.833
-
phenylglyoxalate pH 6.9, 85°C, under argon Thermococcus litoralis
1.2.7.7 0.933
-
2-ketocaproate pH 6.9, 85°C, under argon Pyrococcus sp.
1.2.7.7 1
-
2-ketocaproate pH 6.9, 85°C, under argon Thermococcus litoralis
1.2.7.7 1.07
-
3-methyl-2-oxobutanoate pH 6.9, 85°C, under argon Pyrococcus sp.
1.2.7.7 1.27
-
2-keto-3-methylvalerate pH 6.9, 85°C, under argon Thermococcus litoralis
1.2.7.7 1.33
-
phenylglyoxylate pH 6.9, 85°C, under argon Pyrococcus sp.
1.2.7.7 1.47
-
2-Ketobutyrate pH 6.9, 85°C, under argon Pyrococcus sp.
1.2.7.7 1.53
-
oxidized ferredoxin pH 6.9, 85°C, under argon Thermococcus litoralis
1.2.7.7 1.73
-
pyruvate pH 6.9, 85°C, under argon Pyrococcus sp.
1.2.7.7 2
-
2-ketoisocaproate pH 6.9, 85°C, under argon Thermococcus litoralis
1.2.7.7 2.07
-
2-keto-3-methylvalerate pH 6.9, 85°C, under argon Pyrococcus sp.
1.2.7.7 2.47
-
2-ketoisocaproate pH 6.9, 85°C, under argon Pyrococcus sp.
1.2.7.7 3
-
2-ketoisovalerate pH 6.9, 85°C, under argon Thermococcus litoralis
1.2.7.7 3.67
-
2-ketoisovalerate pH 6.9, 85°C, under argon Pyrococcus sp.
1.2.7.7 5
-
CoA pH 6.9, 85°C, under argon Thermococcus litoralis
1.2.7.7 5.6
-
CoA pH 6.9, 85°C, under argon Pyrococcus sp.
1.2.7.7 22.3
-
2-Ketobutyrate pH 6.9, 85°C, under argon Thermococcus litoralis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.7.7 7
-
-
Thermococcus litoralis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.2.7.7 6 8 pH 6.0: less than 50% loss of activity, pH 8.0: 25% loss of activity Thermococcus litoralis