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Literature summary extracted from

  • Mukund, S.; Adams, M.W.
    Glyceraldehyde-3-phosphate ferredoxin oxidoreductase, a novel tungsten-containing enzyme with a potential glycolytic role in the hyperthermophilic archaeon Pyrococcus furiosus (1995), J. Biol. Chem., 270, 8389-8392.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.7.6 2,3-bisphosphoglycerate stimulates Pyrococcus furiosus
1.2.7.6 3-phosphoglycerate stimulates Pyrococcus furiosus
1.2.7.6 acetyl phosphate stimulates Pyrococcus furiosus
1.2.7.6 Ionic strength particularly by polyanions Pyrococcus furiosus
1.2.7.6 potassium chloride 3.5fold activation at 200 mM Pyrococcus furiosus
1.2.7.6 potassium phosphate 3.5fold activation at 200 mM Pyrococcus furiosus
1.2.7.6 Sodium arsenate 3.5fold activation at 200 mM Pyrococcus furiosus
1.2.7.6 Sodium citrate 3.5fold activation at 200 mM Pyrococcus furiosus
1.2.7.6 Sodium sulfate 3.5fold activation at 200 mM Pyrococcus furiosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.7.6 crotonaldehyde 25% inhibition at 10 mM Pyrococcus furiosus
1.2.7.6 Dithionite 50% inhibition at 3 mM, pH 8.0; O2-scavenger; reversible by 70% Pyrococcus furiosus
1.2.7.6 formaldehyde 25% inhibition at 10 mM Pyrococcus furiosus
1.2.7.6 glyceraldehyde-3-phosphate substrate inhibition above 0.5 mM Pyrococcus furiosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.7.6 0.006
-
 oxidized ferredoxin pH 8.4, 70°C Pyrococcus furiosus
1.2.7.6 0.028
-
 D-Glyceraldehyde-3-phosphate with ferredoxin as electron acceptor, pH 8.4, 70°C Pyrococcus furiosus
1.2.7.6 0.043
-
 D-Glyceraldehyde-3-phosphate with benzyl viologen as electron acceptor, pH 8.4, 70°C Pyrococcus furiosus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.7.6 cytosol
-
 Pyrococcus furiosus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.7.6 Fe2+ enzyme contains iron-sulfur cluster Pyrococcus furiosus
1.2.7.6 Fe2+ 5-6 iron g-atoms per mol of enzyme Pyrococcus furiosus
1.2.7.6 Tungsten 0.85 tungsten g-atoms per mol of enzyme, part of a tungsten-molybdopterin cofactor Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.7.6 60000
-
 gel filtration Pyrococcus furiosus
1.2.7.6 63000
-
 1 * 63000, SDS-PAGE Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.7.6 D-glyceraldehyde-3-phosphate + H2O + oxidized ferredoxin Pyrococcus furiosus might have a glycolytic role and functions in place of glyceraldehyde-3-phosphate dehydrogenase and possibly phosphoglycerate kinase 3-phospho-D-glycerate + H+ + reduced ferredoxin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.6 Pyrococcus furiosus
-
 hyperthermophilic archaeon
-
1.2.7.6 Pyrococcus furiosus
-
 strain DSM 3638
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.2.7.6 aerobic conditions: loss of about 50% activity in cell extract after 12 h at 23°C Pyrococcus furiosus
1.2.7.6 sensitive against O2 Pyrococcus furiosus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.7.6 30fold, under strictly anaerobic conditions Pyrococcus furiosus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.2.7.6 D-glyceraldehyde-3-phosphate + H2O + oxidized ferredoxin = 3-phospho-D-glycerate + 2 H+ + reduced ferredoxin this enzyme is thought to function in place of glyceraldehyde-3-phosphate dehydrogenase and possibly phosphoglycerate kinase in the novel Embden-Meyerhoff-type glycolytic pathway found in Pyrococcus furiosus. It is specific for glyceraldehyde-3-phosphate Pyrococcus furiosus
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.7.6 140
-
 purified enzyme Pyrococcus furiosus

Storage Stability

EC Number Storage Stability Organism
1.2.7.6 23°C, under argon, 12 h, no loss of activity in cell extract Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.6 D-glyceraldehyde-3-phosphate + H2O + oxidized benzyl viologen artificial electron acceptor Pyrococcus furiosus 3-phospho-D-glycerate + H+ + reduced benzyl viologen
-
 ?
1.2.7.6 D-glyceraldehyde-3-phosphate + H2O + oxidized ferredoxin
-
 Pyrococcus furiosus 3-phospho-D-glycerate + H+ + reduced ferredoxin
-
 ?
1.2.7.6 D-glyceraldehyde-3-phosphate + H2O + oxidized ferredoxin might have a glycolytic role and functions in place of glyceraldehyde-3-phosphate dehydrogenase and possibly phosphoglycerate kinase Pyrococcus furiosus 3-phospho-D-glycerate + H+ + reduced ferredoxin
-
 ?
1.2.7.6 additional information no activity with NAD(P)+ as electron acceptor Pyrococcus furiosus ?
-
 ?
1.2.7.6 additional information no activity with formaldehyde, propionaldehyde, phenylacetaldehyde, butyraldehyde, crotonaldehyde, acetaldehyde, dihydroxyacetone phosphate, glyceraldehyde, benzaldehyde, glucose, glucose 6-phosphate, or glyoxylate Pyrococcus furiosus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.7.6 monomer 1 * 63000, SDS-PAGE Pyrococcus furiosus

Synonyms

EC Number Synonyms Comment Organism
1.2.7.6 glyceraldehyde-3-phosphate ferredoxin oxidoreductase
-
 Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.7.6 70
-
 assay at Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.7.6 8.4
-
 assay at Pyrococcus furiosus

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.7.6 Ferredoxin
-
 Pyrococcus furiosus
1.2.7.6 tungsten-molybdopterin tungstopterin center Pyrococcus furiosus