EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.2.7.B2 | the enzyme is crystallized at room temperature under an argon atmosphere using a modification of the melting-point capillary method in the native state and as a complex with the inhibitor glutarate at 1.85 A and 2.4 A resolution. The binding site on formaldehyde ferredoxin oxidoreductase for the physiological electron acceptor, Pyrococcus furiosus ferredoxin is established from an formaldehyde ferredoxin oxidoreductase/ferredoxin cocrystal structure | Pyrococcus furiosus |
1.2.7.5 | formaldehyde ferredoxin oxidoreductase, purified enzyme, modified melting-point capillary method, room temperature under argon atmosphere, protein solution: 55-65 mg/ml, 50 mM Tris-HCl, pH 8.0, 2 mM dithionite, 2 mM DTT, 0.2 M KCl, precipitant solution: 30% v/v glycerol, 20% w/v PEG 4000, 0.1 M sodium citrate, pH 5.6, 0.2 M NaCl, several weeks, X-ray structure determination and analysis | Pyrococcus furiosus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.2.7.B2 | Iron | an electron transfer pathway is proposed that begins at the tungsten center, leads to the (4Fe-4S) cluster of formaldehyde ferredoxin oxidoreductase via one of the two pterins that coordinate the tungsten, and ends at the (4Fe-4S) cluster of ferredoxin. This pathway includes two residues that coordinate the (4Fe-4S) clusters, Cys287 of FOR and Asp14 of ferredoxin | Pyrococcus furiosus | |
1.2.7.B2 | Tungsten | an electron transfer pathway is proposed that begins at the tungsten center, leads to the (4Fe-4S) cluster of formaldehyde ferredoxin oxidoreductase via one of the two pterins that coordinate the tungsten, and ends at the (4Fe-4S) cluster of ferredoxin. This pathway includes two residues that coordinate the (4Fe-4S) clusters, Cys287 of FOR and Asp14 of ferredoxin | Pyrococcus furiosus | |
1.2.7.5 | Ca2+ | binding site, formaldehyde ferredoxin oxidoreductase | Pyrococcus furiosus | |
1.2.7.5 | Fe2+ | contains 1 iron-sulfur cluster [4Fe-4S] per subunit | Pyrococcus furiosus | |
1.2.7.5 | Fe2+ | the [4Fe-4S]-cluster is coordinated by Cys287 of the formaldehyde ferredoxin oxidoreductase and Asp14 of ferredoxin, structure | Pyrococcus furiosus | |
1.2.7.5 | Tungsten | - |
Pyrococcus furiosus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.7.B2 | Pyrococcus furiosus | Q8U1K3 | - |
- |
1.2.7.5 | Pyrococcus furiosus | - |
hyperthermophilic archaeon | - |
1.2.7.5 | Pyrococcus furiosus | - |
Protein Data Bank entries 1B25 and 1B4N | - |
1.2.7.5 | Pyrococcus furiosus | - |
contains a formaldehyde and an aldehyde ferredoxin oxidoreductase | - |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.2.7.5 | an aldehyde + H2O + oxidized ferredoxin = a carboxylate + 2 H+ + reduced ferredoxin | active site structure | Pyrococcus furiosus | |
1.2.7.5 | an aldehyde + H2O + oxidized ferredoxin = a carboxylate + 2 H+ + reduced ferredoxin | intermolecular interaction analysis | Pyrococcus furiosus | |
1.2.7.5 | an aldehyde + H2O + oxidized ferredoxin = a carboxylate + 2 H+ + reduced ferredoxin | substrate binding, catalytic site and reaction mechanism including cofactor action | Pyrococcus furiosus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.7.5 | an aldehyde + H2O + oxidized ferredoxin | formaldehyde ferredoxin oxidoreductase, C1-C3 aldehydes | Pyrococcus furiosus | an acid + H+ + reduced ferredoxin | - |
ir |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.7.5 | More | formaldehyde ferredoxin oxidoreductase and aldehyde ferredoxin oxidoreductase are 2 separate enzymes | Pyrococcus furiosus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.7.B2 | pterin | an electron transfer pathway is proposed that begins at the tungsten center, leads to the (4Fe-4S) cluster of formaldehyde ferredoxin oxidoreductase via one of the two pterins that coordinate the tungsten, and ends at the (4Fe-4S) cluster of ferredoxin. This pathway includes two residues that coordinate the (4Fe4S) clusters, Cys287 of FOR and Asp14 of ferredoxin | Pyrococcus furiosus | |
1.2.7.5 | Ferredoxin | formaldehyde ferredoxin oxidoreductase, binding mechanism | Pyrococcus furiosus | |
1.2.7.5 | tungsten cofactor | formaldehyde ferredoxin oxidoreductase | Pyrococcus furiosus | |
1.2.7.5 | tungsten-molybdopterin | tungstopterin center | Pyrococcus furiosus |