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Literature summary extracted from

  • Hu, Y.; Faham, S.; Roy, R.; Adams, M.W.; Rees, D.C.
    Formaldehyde ferredoxin oxidoreductase from Pyrococcus furiosus: the 1.85 A resolution crystal structure and its mechanistic implications (1999), J. Mol. Biol., 286, 899-914.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.2.7.B2 the enzyme is crystallized at room temperature under an argon atmosphere using a modification of the melting-point capillary method in the native state and as a complex with the inhibitor glutarate at 1.85 A and 2.4 A resolution. The binding site on formaldehyde ferredoxin oxidoreductase for the physiological electron acceptor, Pyrococcus furiosus ferredoxin is established from an formaldehyde ferredoxin oxidoreductase/ferredoxin cocrystal structure Pyrococcus furiosus
1.2.7.5 formaldehyde ferredoxin oxidoreductase, purified enzyme, modified melting-point capillary method, room temperature under argon atmosphere, protein solution: 55-65 mg/ml, 50 mM Tris-HCl, pH 8.0, 2 mM dithionite, 2 mM DTT, 0.2 M KCl, precipitant solution: 30% v/v glycerol, 20% w/v PEG 4000, 0.1 M sodium citrate, pH 5.6, 0.2 M NaCl, several weeks, X-ray structure determination and analysis Pyrococcus furiosus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.7.B2 Iron an electron transfer pathway is proposed that begins at the tungsten center, leads to the (4Fe-4S) cluster of formaldehyde ferredoxin oxidoreductase via one of the two pterins that coordinate the tungsten, and ends at the (4Fe-4S) cluster of ferredoxin. This pathway includes two residues that coordinate the (4Fe-4S) clusters, Cys287 of FOR and Asp14 of ferredoxin Pyrococcus furiosus
1.2.7.B2 Tungsten an electron transfer pathway is proposed that begins at the tungsten center, leads to the (4Fe-4S) cluster of formaldehyde ferredoxin oxidoreductase via one of the two pterins that coordinate the tungsten, and ends at the (4Fe-4S) cluster of ferredoxin. This pathway includes two residues that coordinate the (4Fe-4S) clusters, Cys287 of FOR and Asp14 of ferredoxin Pyrococcus furiosus
1.2.7.5 Ca2+ binding site, formaldehyde ferredoxin oxidoreductase Pyrococcus furiosus
1.2.7.5 Fe2+ contains 1 iron-sulfur cluster [4Fe-4S] per subunit Pyrococcus furiosus
1.2.7.5 Fe2+ the [4Fe-4S]-cluster is coordinated by Cys287 of the formaldehyde ferredoxin oxidoreductase and Asp14 of ferredoxin, structure Pyrococcus furiosus
1.2.7.5 Tungsten
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Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.B2 Pyrococcus furiosus Q8U1K3
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1.2.7.5 Pyrococcus furiosus
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hyperthermophilic archaeon
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1.2.7.5 Pyrococcus furiosus
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Protein Data Bank entries 1B25 and 1B4N
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1.2.7.5 Pyrococcus furiosus
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contains a formaldehyde and an aldehyde ferredoxin oxidoreductase
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Reaction

EC Number Reaction Comment Organism Reaction ID
1.2.7.5 an aldehyde + H2O + oxidized ferredoxin = a carboxylate + 2 H+ + reduced ferredoxin active site structure Pyrococcus furiosus
1.2.7.5 an aldehyde + H2O + oxidized ferredoxin = a carboxylate + 2 H+ + reduced ferredoxin intermolecular interaction analysis Pyrococcus furiosus
1.2.7.5 an aldehyde + H2O + oxidized ferredoxin = a carboxylate + 2 H+ + reduced ferredoxin substrate binding, catalytic site and reaction mechanism including cofactor action Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.5 an aldehyde + H2O + oxidized ferredoxin formaldehyde ferredoxin oxidoreductase, C1-C3 aldehydes Pyrococcus furiosus an acid + H+ + reduced ferredoxin
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ir

Synonyms

EC Number Synonyms Comment Organism
1.2.7.5 More formaldehyde ferredoxin oxidoreductase and aldehyde ferredoxin oxidoreductase are 2 separate enzymes Pyrococcus furiosus

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.7.B2 pterin an electron transfer pathway is proposed that begins at the tungsten center, leads to the (4Fe-4S) cluster of formaldehyde ferredoxin oxidoreductase via one of the two pterins that coordinate the tungsten, and ends at the (4Fe-4S) cluster of ferredoxin. This pathway includes two residues that coordinate the (4Fe4S) clusters, Cys287 of FOR and Asp14 of ferredoxin Pyrococcus furiosus
1.2.7.5 Ferredoxin formaldehyde ferredoxin oxidoreductase, binding mechanism Pyrococcus furiosus
1.2.7.5 tungsten cofactor formaldehyde ferredoxin oxidoreductase Pyrococcus furiosus
1.2.7.5 tungsten-molybdopterin tungstopterin center Pyrococcus furiosus