BRENDA - Enzyme Database show

Purification and molecular characterization of the tungsten-containing formaldehyde ferredoxin oxidoreductase from the hyperthermophilic archaeon Pyrococcus furiosus: the third of a putative five-member tungstoenzyme family

Roy, R.; Mukund, S.; Schut, G.J.; Dunn, D.M.; Weiss, R.; Adams, M.W.; J. Bacteriol. 181, 1171-1180 (1999)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.2.7.B2
Sulfide
activated up to fivefold by treatment with sulfide under reducing conditions
Pyrococcus furiosus
1.2.7.5
Sulfide
activation of formaldehyde ferredoxin oxidoreductase
Pyrococcus furiosus
General Stability
EC Number
General Stability
Organism
1.2.7.5
10% glycerol and 2 mM DTT stabilize during purification
Pyrococcus furiosus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.7.B2
glycerol
60% v/v, 6 h, 60% loss of activity
Pyrococcus furiosus
1.2.7.B2
Glyoxal
above 1 mM
Pyrococcus furiosus
1.2.7.B2
additional information
the enzyme is not inhibited when it is incubated for up to 24 h with iodoacetate, arsenite, or cyanide (each at a concentration of 5 mM) prior to being assayed under standard conditions in the absence of these reagents
Pyrococcus furiosus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.7.B2
0.1
-
oxidized ferredoxin
80°C, pH 8.4
Pyrococcus furiosus
1.2.7.B2
0.8
-
glutaric dialdehyde
80°C, pH 8.4
Pyrococcus furiosus
1.2.7.B2
8
-
Succinic semialdehyde
80°C, pH 8.4
Pyrococcus furiosus
1.2.7.B2
15
-
phenylpropionaldehyde
80°C, pH 8.4
Pyrococcus furiosus
1.2.7.B2
25
-
formaldehyde
80°C, pH 8.4
Pyrococcus furiosus
1.2.7.B2
25
-
Indole-3-acetaldehyde
80°C, pH 8.4
Pyrococcus furiosus
1.2.7.B2
60
-
acetaldehyde
80°C, pH 8.4
Pyrococcus furiosus
1.2.7.B2
62
-
propionaldehyde
80°C, pH 8.4
Pyrococcus furiosus
1.2.7.5
additional information
-
additional information
formaldehyde ferredoxin oxidoreductase, Km for several substrates at different concentrations with purified and sulfide-activated enzyme, overview
Pyrococcus furiosus
1.2.7.5
25
-
formaldehyde
formaldehyde ferredoxin oxidoreductase, pH 8.4, 80°C, with benzyl viologen as electron acceptor
Pyrococcus furiosus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.2.7.B2
Calcium
contains approximately 1 W atom, 4 Fe atoms, and 1 Ca atom per subunit
Pyrococcus furiosus
1.2.7.B2
Iron
contains approximately 1 W atom, 4 Fe atoms, and 1 Ca atom per subunit
Pyrococcus furiosus
1.2.7.B2
Tungsten
tungsten-containing enzyme. The enzyme contains approximately 1 W atom, 4 Fe atoms, and 1 Ca atom per subunit
Pyrococcus furiosus
1.2.7.5
Ca2+
0.4 mol per mol of subunit, formaldehyde ferredoxin oxidoreductase
Pyrococcus furiosus
1.2.7.5
Fe2+
3.8 gatoms per subunit, formaldehyde ferredoxin oxidoreductase
Pyrococcus furiosus
1.2.7.5
Mg2+
1.5 mol per mol of subunit, formaldehyde ferredoxin oxidoreductase
Pyrococcus furiosus
1.2.7.5
Tungsten
0.9 gatom per subunit, formaldehyde ferredoxin oxidoreductase
Pyrococcus furiosus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.7.B2
68700
-
4 * 68700, calculated from sequence
Pyrococcus furiosus
1.2.7.B2
275000
-
gel filtration
Pyrococcus furiosus
1.2.7.5
68000
-
4 * 68000, formaldehyde ferredoxin oxidoreductase, SDS-PAGE
Pyrococcus furiosus
1.2.7.5
275000
-
formaldehyde ferredoxin oxidoreductase, gel filtration
Pyrococcus furiosus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.7.5
an aldehyde + H2O + oxidized ferredoxin
Pyrococcus furiosus
-
an acid + H+ + reduced ferredoxin
-
Pyrococcus furiosus
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.7.B2
Pyrococcus furiosus
O93738
-
-
1.2.7.5
Pyrococcus furiosus
-
contains a formaldehyde and an aldehyde ferredoxin oxidoreductase; genes aor and for; hyperthermophilic archaeon; strain DSM 3638
-
Oxidation Stability
EC Number
Oxidation Stability
Organism
1.2.7.B2
enzyme as purified under standard conditions is oxygen sensitive, with the t50% being about 12 h when the enzyme is exposed to air. No activity is lost under anaerobic conditions
Pyrococcus furiosus
Purification (Commentary)
EC Number
Commentary
Organism
1.2.7.B2
-
Pyrococcus furiosus
1.2.7.5
formaldehyde ferredoxin oxidoreductase, 17fold
Pyrococcus furiosus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2.7.B2
58
-
pH 8.4, 90°C
Pyrococcus furiosus
1.2.7.5
additional information
-
formaldehyde ferredoxin oxidoreductase, substrate specificity
Pyrococcus furiosus
1.2.7.5
42
-
purified formaldehyde ferredoxin oxidoreductase
Pyrococcus furiosus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.7.B2
acetaldehyde + H2O + oxidized benzyl viologen
-
644689
Pyrococcus furiosus
acetate + reduced benzyl viologen + H+
-
-
-
?
1.2.7.B2
formaldehyde + H2O + 2 oxidized ferredoxin
formaldehyde is unlikely to be the physiologiocal substrate (Km-value: 25 mM)
644689
Pyrococcus furiosus
formate + 2 reduced ferredoxin + 2 H+
-
-
-
?
1.2.7.B2
formaldehyde + H2O + oxidized benzyl viologen
-
644689
Pyrococcus furiosus
formate + reduced benzyl viologen + H+
-
-
-
?
1.2.7.B2
glutaric dialdehyde + H2O + oxidized benzyl viologen
best substrate, C5- or C6-type aldehydes might be physiologically relevant substrates
644689
Pyrococcus furiosus
? + reduced benzyl viologen + H+
-
-
-
?
1.2.7.B2
indole-3-acetaldehyde + H2O + oxidized benzyl viologen
-
644689
Pyrococcus furiosus
indole-3-acetate + reduced benzyl viologen + H+
-
-
-
?
1.2.7.B2
additional information
low activity with butyraldehyde, crotonaldehyde, no activity with: isovaleraldehyde, benzaldehyde, salicaldehyde, 2-furfuraldehyde. It does not oxidize lactate, 2-hydroxybutyrate, 2-hydroxyvalerate, 2-hydroxycaproate, or 1-ethyl-2-hydroxycaproate and it does not reduce pyruvate, 2-oxobutyrate, 2-oxovalerate, 1-ethyl-3-oxovalerate, 1-ethyl-4-oxovalerate, or 2-oxocaproate
644689
Pyrococcus furiosus
?
-
-
-
-
1.2.7.B2
phenylpropionaldehyde + H2O + oxidized benzyl viologen
-
644689
Pyrococcus furiosus
phenylpropionate + reduced benzyl viologen + H+
-
-
-
?
1.2.7.B2
propionaldehyde + H2O + oxidized benzyl viologen
-
644689
Pyrococcus furiosus
propionate + reduced benzyl viologen + H+
-
-
-
?
1.2.7.B2
succinic semialdehyde + H2O + oxidized benzyl viologen
-
644689
Pyrococcus furiosus
succinate + reduced benzyl viologen + H+
-
-
-
?
1.2.7.5
acetaldehyde + H2O + oxidized benzyl viologen
formaldehyde ferredoxin oxidoreductase
644689
Pyrococcus furiosus
acetate + H+ + reduced benzyl viologen
-
644689
Pyrococcus furiosus
ir
1.2.7.5
acetaldehyde + H2O + oxidized ferredoxin
formaldehyde ferredoxin oxidoreductase
644689
Pyrococcus furiosus
acetate + H+ + reduced ferredoxin
-
644689
Pyrococcus furiosus
ir
1.2.7.5
an aldehyde + H2O + oxidized ferredoxin
-
644689
Pyrococcus furiosus
an acid + H+ + reduced ferredoxin
-
644689
Pyrococcus furiosus
?
1.2.7.5
an aldehyde + H2O + oxidized ferredoxin
formaldehyde ferredoxin oxidoreductase, C1-C3 aldehydes
644689
Pyrococcus furiosus
an acid + H+ + reduced ferredoxin
-
644689
Pyrococcus furiosus
ir
1.2.7.5
butyraldehyde + H2O + oxidized ferredoxin
low activity
644689
Pyrococcus furiosus
butyrate + H+ + reduced ferredoxin
-
644689
Pyrococcus furiosus
ir
1.2.7.5
butyraldehyde + H2O + oxidized ferredoxin
formaldehyde ferredoxin oxidoreductase
644689
Pyrococcus furiosus
butyrate + H+ + reduced ferredoxin
-
644689
Pyrococcus furiosus
ir
1.2.7.5
crotonaldehyde + H2O + oxidized benzyl viologen
low activity, formaldehyde ferredoxin oxidoreductase
644689
Pyrococcus furiosus
pyruvate + H+ + reduced benzyl viologen
-
-
-
ir
1.2.7.5
formaldehyde + H2O + oxidized benzyl viologen
formaldehyde ferredoxin oxidoreductase
644689
Pyrococcus furiosus
formate + H+ + reduced benzyl viologen
-
644689
Pyrococcus furiosus
ir
1.2.7.5
formaldehyde + H2O + oxidized ferredoxin
formaldehyde and aldehyde ferredoxin oxidoreductases
644689
Pyrococcus furiosus
formate + H+ + reduced ferredoxin
-
644689
Pyrococcus furiosus
ir
1.2.7.5
isovalerylaldehyde + H2O + oxidized benzyl viologen
formaldehyde ferredoxin oxidoreductase, no activity
644689
Pyrococcus furiosus
isovalerate + H+ + reduced benzyl viologen
-
-
-
-
1.2.7.5
propionaldehyde + H2O + oxidized benzyl viologen
formaldehyde ferredoxin oxidoreductase
644689
Pyrococcus furiosus
propionate + H+ + reduced benzyl viologen
-
644689
Pyrococcus furiosus
ir
1.2.7.5
propionaldehyde + H2O + oxidized ferredoxin
formaldehyde ferredoxin oxidoreductase
644689
Pyrococcus furiosus
propionate + H+ + reduced ferredoxin
-
644689
Pyrococcus furiosus
ir
Subunits
EC Number
Subunits
Commentary
Organism
1.2.7.B2
homotetramer
4 * 68700, calculated from sequence
Pyrococcus furiosus
1.2.7.5
tetramer
4 * 68000, formaldehyde ferredoxin oxidoreductase, SDS-PAGE
Pyrococcus furiosus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.2.7.5
80
-
assay at
Pyrococcus furiosus
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.2.7.5
60
90
formaldehyde ferredoxin oxidoreductase, activity increases 4.5fold from 60°C to 90°C at pH 8.4
Pyrococcus furiosus
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.2.7.B2
80
-
8 h, 50% loss of activity
Pyrococcus furiosus
1.2.7.5
additional information
-
the enzyme is thermostable
Pyrococcus furiosus
1.2.7.5
80
-
formaldehyde ferredoxin oxidoreductase, t1/2: 8 h, in presence of 2 mM dithionite and 2 mM DTT, pH 8.4
Pyrococcus furiosus
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.2.7.B2
2300
-
Indole-3-acetaldehyde
80°C, pH 8.4
Pyrococcus furiosus
1.2.7.B2
5700
-
Succinic semialdehyde
80°C, pH 8.4
Pyrococcus furiosus
1.2.7.B2
11000
-
propionaldehyde
80°C, pH 8.4
Pyrococcus furiosus
1.2.7.B2
25000
-
phenylpropionaldehyde
80°C, pH 8.4
Pyrococcus furiosus
1.2.7.B2
42000
-
glutaric dialdehyde
80°C, pH 8.4
Pyrococcus furiosus
1.2.7.B2
44000
-
acetaldehyde
80°C, pH 8.4
Pyrococcus furiosus
1.2.7.B2
71000
-
formaldehyde
80°C, pH 8.4
Pyrococcus furiosus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.7.B2
10
-
the activity of the pure enzyme with benzyl viologen as the electron acceptor increases linearly with pH over the pH range 5.5 (1.5 U/mg) to 10.0 (55 U/mg) at 80°C
Pyrococcus furiosus
1.2.7.5
8.4
-
assay at
Pyrococcus furiosus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.2.7.B2
5.5
10
the activity of the pure enzyme with benzyl viologen as the electron acceptor increases linearly with pH over the pH range 5.5 (1.5 U/mg) to 10.0 (55 U/mg) at 80°C
Pyrococcus furiosus
1.2.7.5
5.5
10
formaldehyde ferredoxin oxidoreductase, activity increases linearly from pH 5.5 to pH 10.0 at 80°C
Pyrococcus furiosus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.2.7.B2
additional information
the enzyme contains a pterin cofactor
Pyrococcus furiosus
1.2.7.5
Ferredoxin
-
Pyrococcus furiosus
1.2.7.5
form A cofactor
formaldehyde ferredoxin oxidoreductase
Pyrococcus furiosus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.2.7.B2
Sulfide
activated up to fivefold by treatment with sulfide under reducing conditions
Pyrococcus furiosus
1.2.7.5
Sulfide
activation of formaldehyde ferredoxin oxidoreductase
Pyrococcus furiosus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.2.7.B2
additional information
the enzyme contains a pterin cofactor
Pyrococcus furiosus
1.2.7.5
Ferredoxin
-
Pyrococcus furiosus
1.2.7.5
form A cofactor
formaldehyde ferredoxin oxidoreductase
Pyrococcus furiosus
General Stability (protein specific)
EC Number
General Stability
Organism
1.2.7.5
10% glycerol and 2 mM DTT stabilize during purification
Pyrococcus furiosus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.7.B2
glycerol
60% v/v, 6 h, 60% loss of activity
Pyrococcus furiosus
1.2.7.B2
Glyoxal
above 1 mM
Pyrococcus furiosus
1.2.7.B2
additional information
the enzyme is not inhibited when it is incubated for up to 24 h with iodoacetate, arsenite, or cyanide (each at a concentration of 5 mM) prior to being assayed under standard conditions in the absence of these reagents
Pyrococcus furiosus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.7.B2
0.1
-
oxidized ferredoxin
80°C, pH 8.4
Pyrococcus furiosus
1.2.7.B2
0.8
-
glutaric dialdehyde
80°C, pH 8.4
Pyrococcus furiosus
1.2.7.B2
8
-
Succinic semialdehyde
80°C, pH 8.4
Pyrococcus furiosus
1.2.7.B2
15
-
phenylpropionaldehyde
80°C, pH 8.4
Pyrococcus furiosus
1.2.7.B2
25
-
formaldehyde
80°C, pH 8.4
Pyrococcus furiosus
1.2.7.B2
25
-
Indole-3-acetaldehyde
80°C, pH 8.4
Pyrococcus furiosus
1.2.7.B2
60
-
acetaldehyde
80°C, pH 8.4
Pyrococcus furiosus
1.2.7.B2
62
-
propionaldehyde
80°C, pH 8.4
Pyrococcus furiosus
1.2.7.5
additional information
-
additional information
formaldehyde ferredoxin oxidoreductase, Km for several substrates at different concentrations with purified and sulfide-activated enzyme, overview
Pyrococcus furiosus
1.2.7.5
25
-
formaldehyde
formaldehyde ferredoxin oxidoreductase, pH 8.4, 80°C, with benzyl viologen as electron acceptor
Pyrococcus furiosus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.2.7.B2
Calcium
contains approximately 1 W atom, 4 Fe atoms, and 1 Ca atom per subunit
Pyrococcus furiosus
1.2.7.B2
Iron
contains approximately 1 W atom, 4 Fe atoms, and 1 Ca atom per subunit
Pyrococcus furiosus
1.2.7.B2
Tungsten
tungsten-containing enzyme. The enzyme contains approximately 1 W atom, 4 Fe atoms, and 1 Ca atom per subunit
Pyrococcus furiosus
1.2.7.5
Ca2+
0.4 mol per mol of subunit, formaldehyde ferredoxin oxidoreductase
Pyrococcus furiosus
1.2.7.5
Fe2+
3.8 gatoms per subunit, formaldehyde ferredoxin oxidoreductase
Pyrococcus furiosus
1.2.7.5
Mg2+
1.5 mol per mol of subunit, formaldehyde ferredoxin oxidoreductase
Pyrococcus furiosus
1.2.7.5
Tungsten
0.9 gatom per subunit, formaldehyde ferredoxin oxidoreductase
Pyrococcus furiosus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.7.B2
68700
-
4 * 68700, calculated from sequence
Pyrococcus furiosus
1.2.7.B2
275000
-
gel filtration
Pyrococcus furiosus
1.2.7.5
68000
-
4 * 68000, formaldehyde ferredoxin oxidoreductase, SDS-PAGE
Pyrococcus furiosus
1.2.7.5
275000
-
formaldehyde ferredoxin oxidoreductase, gel filtration
Pyrococcus furiosus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.7.5
an aldehyde + H2O + oxidized ferredoxin
Pyrococcus furiosus
-
an acid + H+ + reduced ferredoxin
-
Pyrococcus furiosus
?
Oxidation Stability (protein specific)
EC Number
Oxidation Stability
Organism
1.2.7.B2
enzyme as purified under standard conditions is oxygen sensitive, with the t50% being about 12 h when the enzyme is exposed to air. No activity is lost under anaerobic conditions
Pyrococcus furiosus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.7.B2
-
Pyrococcus furiosus
1.2.7.5
formaldehyde ferredoxin oxidoreductase, 17fold
Pyrococcus furiosus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2.7.B2
58
-
pH 8.4, 90°C
Pyrococcus furiosus
1.2.7.5
additional information
-
formaldehyde ferredoxin oxidoreductase, substrate specificity
Pyrococcus furiosus
1.2.7.5
42
-
purified formaldehyde ferredoxin oxidoreductase
Pyrococcus furiosus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.7.B2
acetaldehyde + H2O + oxidized benzyl viologen
-
644689
Pyrococcus furiosus
acetate + reduced benzyl viologen + H+
-
-
-
?
1.2.7.B2
formaldehyde + H2O + 2 oxidized ferredoxin
formaldehyde is unlikely to be the physiologiocal substrate (Km-value: 25 mM)
644689
Pyrococcus furiosus
formate + 2 reduced ferredoxin + 2 H+
-
-
-
?
1.2.7.B2
formaldehyde + H2O + oxidized benzyl viologen
-
644689
Pyrococcus furiosus
formate + reduced benzyl viologen + H+
-
-
-
?
1.2.7.B2
glutaric dialdehyde + H2O + oxidized benzyl viologen
best substrate, C5- or C6-type aldehydes might be physiologically relevant substrates
644689
Pyrococcus furiosus
? + reduced benzyl viologen + H+
-
-
-
?
1.2.7.B2
indole-3-acetaldehyde + H2O + oxidized benzyl viologen
-
644689
Pyrococcus furiosus
indole-3-acetate + reduced benzyl viologen + H+
-
-
-
?
1.2.7.B2
additional information
low activity with butyraldehyde, crotonaldehyde, no activity with: isovaleraldehyde, benzaldehyde, salicaldehyde, 2-furfuraldehyde. It does not oxidize lactate, 2-hydroxybutyrate, 2-hydroxyvalerate, 2-hydroxycaproate, or 1-ethyl-2-hydroxycaproate and it does not reduce pyruvate, 2-oxobutyrate, 2-oxovalerate, 1-ethyl-3-oxovalerate, 1-ethyl-4-oxovalerate, or 2-oxocaproate
644689
Pyrococcus furiosus
?
-
-
-
-
1.2.7.B2
phenylpropionaldehyde + H2O + oxidized benzyl viologen
-
644689
Pyrococcus furiosus
phenylpropionate + reduced benzyl viologen + H+
-
-
-
?
1.2.7.B2
propionaldehyde + H2O + oxidized benzyl viologen
-
644689
Pyrococcus furiosus
propionate + reduced benzyl viologen + H+
-
-
-
?
1.2.7.B2
succinic semialdehyde + H2O + oxidized benzyl viologen
-
644689
Pyrococcus furiosus
succinate + reduced benzyl viologen + H+
-
-
-
?
1.2.7.5
acetaldehyde + H2O + oxidized benzyl viologen
formaldehyde ferredoxin oxidoreductase
644689
Pyrococcus furiosus
acetate + H+ + reduced benzyl viologen
-
644689
Pyrococcus furiosus
ir
1.2.7.5
acetaldehyde + H2O + oxidized ferredoxin
formaldehyde ferredoxin oxidoreductase
644689
Pyrococcus furiosus
acetate + H+ + reduced ferredoxin
-
644689
Pyrococcus furiosus
ir
1.2.7.5
an aldehyde + H2O + oxidized ferredoxin
-
644689
Pyrococcus furiosus
an acid + H+ + reduced ferredoxin
-
644689
Pyrococcus furiosus
?
1.2.7.5
an aldehyde + H2O + oxidized ferredoxin
formaldehyde ferredoxin oxidoreductase, C1-C3 aldehydes
644689
Pyrococcus furiosus
an acid + H+ + reduced ferredoxin
-
644689
Pyrococcus furiosus
ir
1.2.7.5
butyraldehyde + H2O + oxidized ferredoxin
low activity
644689
Pyrococcus furiosus
butyrate + H+ + reduced ferredoxin
-
644689
Pyrococcus furiosus
ir
1.2.7.5
butyraldehyde + H2O + oxidized ferredoxin
formaldehyde ferredoxin oxidoreductase
644689
Pyrococcus furiosus
butyrate + H+ + reduced ferredoxin
-
644689
Pyrococcus furiosus
ir
1.2.7.5
crotonaldehyde + H2O + oxidized benzyl viologen
low activity, formaldehyde ferredoxin oxidoreductase
644689
Pyrococcus furiosus
pyruvate + H+ + reduced benzyl viologen
-
-
-
ir
1.2.7.5
formaldehyde + H2O + oxidized benzyl viologen
formaldehyde ferredoxin oxidoreductase
644689
Pyrococcus furiosus
formate + H+ + reduced benzyl viologen
-
644689
Pyrococcus furiosus
ir
1.2.7.5
formaldehyde + H2O + oxidized ferredoxin
formaldehyde and aldehyde ferredoxin oxidoreductases
644689
Pyrococcus furiosus
formate + H+ + reduced ferredoxin
-
644689
Pyrococcus furiosus
ir
1.2.7.5
isovalerylaldehyde + H2O + oxidized benzyl viologen
formaldehyde ferredoxin oxidoreductase, no activity
644689
Pyrococcus furiosus
isovalerate + H+ + reduced benzyl viologen
-
-
-
-
1.2.7.5
propionaldehyde + H2O + oxidized benzyl viologen
formaldehyde ferredoxin oxidoreductase
644689
Pyrococcus furiosus
propionate + H+ + reduced benzyl viologen
-
644689
Pyrococcus furiosus
ir
1.2.7.5
propionaldehyde + H2O + oxidized ferredoxin
formaldehyde ferredoxin oxidoreductase
644689
Pyrococcus furiosus
propionate + H+ + reduced ferredoxin
-
644689
Pyrococcus furiosus
ir
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.2.7.B2
homotetramer
4 * 68700, calculated from sequence
Pyrococcus furiosus
1.2.7.5
tetramer
4 * 68000, formaldehyde ferredoxin oxidoreductase, SDS-PAGE
Pyrococcus furiosus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.2.7.5
80
-
assay at
Pyrococcus furiosus
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.2.7.5
60
90
formaldehyde ferredoxin oxidoreductase, activity increases 4.5fold from 60°C to 90°C at pH 8.4
Pyrococcus furiosus
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.2.7.B2
80
-
8 h, 50% loss of activity
Pyrococcus furiosus
1.2.7.5
additional information
-
the enzyme is thermostable
Pyrococcus furiosus
1.2.7.5
80
-
formaldehyde ferredoxin oxidoreductase, t1/2: 8 h, in presence of 2 mM dithionite and 2 mM DTT, pH 8.4
Pyrococcus furiosus
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.2.7.B2
2300
-
Indole-3-acetaldehyde
80°C, pH 8.4
Pyrococcus furiosus
1.2.7.B2
5700
-
Succinic semialdehyde
80°C, pH 8.4
Pyrococcus furiosus
1.2.7.B2
11000
-
propionaldehyde
80°C, pH 8.4
Pyrococcus furiosus
1.2.7.B2
25000
-
phenylpropionaldehyde
80°C, pH 8.4
Pyrococcus furiosus
1.2.7.B2
42000
-
glutaric dialdehyde
80°C, pH 8.4
Pyrococcus furiosus
1.2.7.B2
44000
-
acetaldehyde
80°C, pH 8.4
Pyrococcus furiosus
1.2.7.B2
71000
-
formaldehyde
80°C, pH 8.4
Pyrococcus furiosus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.7.B2
10
-
the activity of the pure enzyme with benzyl viologen as the electron acceptor increases linearly with pH over the pH range 5.5 (1.5 U/mg) to 10.0 (55 U/mg) at 80°C
Pyrococcus furiosus
1.2.7.5
8.4
-
assay at
Pyrococcus furiosus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.2.7.B2
5.5
10
the activity of the pure enzyme with benzyl viologen as the electron acceptor increases linearly with pH over the pH range 5.5 (1.5 U/mg) to 10.0 (55 U/mg) at 80°C
Pyrococcus furiosus
1.2.7.5
5.5
10
formaldehyde ferredoxin oxidoreductase, activity increases linearly from pH 5.5 to pH 10.0 at 80°C
Pyrococcus furiosus
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.2.7.B2
90
-
Indole-3-acetaldehyde
80°C, pH 8.4
Pyrococcus furiosus
1.2.7.B2
170
-
propionaldehyde
80°C, pH 8.4
Pyrococcus furiosus
1.2.7.B2
710
-
Succinic semialdehyde
80°C, pH 8.4
Pyrococcus furiosus
1.2.7.B2
730
-
acetaldehyde
80°C, pH 8.4
Pyrococcus furiosus
1.2.7.B2
1700
-
phenylpropionaldehyde
80°C, pH 8.4
Pyrococcus furiosus
1.2.7.B2
3000
-
formaldehyde
80°C, pH 8.4
Pyrococcus furiosus
1.2.7.B2
60000
-
glutaric dialdehyde
80°C, pH 8.4
Pyrococcus furiosus
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.2.7.B2
90
-
Indole-3-acetaldehyde
80°C, pH 8.4
Pyrococcus furiosus
1.2.7.B2
170
-
propionaldehyde
80°C, pH 8.4
Pyrococcus furiosus
1.2.7.B2
710
-
Succinic semialdehyde
80°C, pH 8.4
Pyrococcus furiosus
1.2.7.B2
730
-
acetaldehyde
80°C, pH 8.4
Pyrococcus furiosus
1.2.7.B2
1700
-
phenylpropionaldehyde
80°C, pH 8.4
Pyrococcus furiosus
1.2.7.B2
3000
-
formaldehyde
80°C, pH 8.4
Pyrococcus furiosus
1.2.7.B2
60000
-
glutaric dialdehyde
80°C, pH 8.4
Pyrococcus furiosus