Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Roy, R.; Mukund, S.; Schut, G.J.; Dunn, D.M.; Weiss, R.; Adams, M.W.
    Purification and molecular characterization of the tungsten-containing formaldehyde ferredoxin oxidoreductase from the hyperthermophilic archaeon Pyrococcus furiosus: the third of a putative five-member tungstoenzyme family (1999), J. Bacteriol., 181, 1171-1180.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.7.B2 Sulfide activated up to fivefold by treatment with sulfide under reducing conditions Pyrococcus furiosus
1.2.7.5 Sulfide activation of formaldehyde ferredoxin oxidoreductase Pyrococcus furiosus

General Stability

EC Number General Stability Organism
1.2.7.5 10% glycerol and 2 mM DTT stabilize during purification Pyrococcus furiosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.7.B2 glycerol 60% v/v, 6 h, 60% loss of activity Pyrococcus furiosus
1.2.7.B2 Glyoxal above 1 mM Pyrococcus furiosus
1.2.7.B2 additional information the enzyme is not inhibited when it is incubated for up to 24 h with iodoacetate, arsenite, or cyanide (each at a concentration of 5 mM) prior to being assayed under standard conditions in the absence of these reagents Pyrococcus furiosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.7.B2 0.1
-
 oxidized ferredoxin 80°C, pH 8.4 Pyrococcus furiosus
1.2.7.B2 0.8
-
 glutaric dialdehyde 80°C, pH 8.4 Pyrococcus furiosus
1.2.7.B2 8
-
 Succinic semialdehyde 80°C, pH 8.4 Pyrococcus furiosus
1.2.7.B2 15
-
 phenylpropionaldehyde 80°C, pH 8.4 Pyrococcus furiosus
1.2.7.B2 25
-
 formaldehyde 80°C, pH 8.4 Pyrococcus furiosus
1.2.7.B2 25
-
 Indole-3-acetaldehyde 80°C, pH 8.4 Pyrococcus furiosus
1.2.7.B2 60
-
 acetaldehyde 80°C, pH 8.4 Pyrococcus furiosus
1.2.7.B2 62
-
 propionaldehyde 80°C, pH 8.4 Pyrococcus furiosus
1.2.7.5 additional information
-
 additional information formaldehyde ferredoxin oxidoreductase, Km for several substrates at different concentrations with purified and sulfide-activated enzyme, overview Pyrococcus furiosus
1.2.7.5 25
-
 formaldehyde formaldehyde ferredoxin oxidoreductase, pH 8.4, 80°C, with benzyl viologen as electron acceptor Pyrococcus furiosus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.7.B2 Calcium contains approximately 1 W atom, 4 Fe atoms, and 1 Ca atom per subunit Pyrococcus furiosus
1.2.7.B2 Iron contains approximately 1 W atom, 4 Fe atoms, and 1 Ca atom per subunit Pyrococcus furiosus
1.2.7.B2 Tungsten tungsten-containing enzyme. The enzyme contains approximately 1 W atom, 4 Fe atoms, and 1 Ca atom per subunit Pyrococcus furiosus
1.2.7.5 Ca2+ 0.4 mol per mol of subunit, formaldehyde ferredoxin oxidoreductase Pyrococcus furiosus
1.2.7.5 Fe2+ 3.8 gatoms per subunit, formaldehyde ferredoxin oxidoreductase Pyrococcus furiosus
1.2.7.5 Mg2+ 1.5 mol per mol of subunit, formaldehyde ferredoxin oxidoreductase Pyrococcus furiosus
1.2.7.5 Tungsten 0.9 gatom per subunit, formaldehyde ferredoxin oxidoreductase Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.7.B2 68700
-
 4 * 68700, calculated from sequence Pyrococcus furiosus
1.2.7.B2 275000
-
 gel filtration Pyrococcus furiosus
1.2.7.5 68000
-
 4 * 68000, formaldehyde ferredoxin oxidoreductase, SDS-PAGE Pyrococcus furiosus
1.2.7.5 275000
-
 formaldehyde ferredoxin oxidoreductase, gel filtration Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.7.5 an aldehyde + H2O + oxidized ferredoxin Pyrococcus furiosus
-
 an acid + H+ + reduced ferredoxin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.B2 Pyrococcus furiosus O93738
-
-
1.2.7.5 Pyrococcus furiosus
-
 hyperthermophilic archaeon
-
1.2.7.5 Pyrococcus furiosus
-
 strain DSM 3638
-
1.2.7.5 Pyrococcus furiosus
-
 contains a formaldehyde and an aldehyde ferredoxin oxidoreductase
-
1.2.7.5 Pyrococcus furiosus
-
 genes aor and for
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.2.7.B2 enzyme as purified under standard conditions is oxygen sensitive, with the t50% being about 12 h when the enzyme is exposed to air. No activity is lost under anaerobic conditions Pyrococcus furiosus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.7.B2
-
 Pyrococcus furiosus
1.2.7.5 formaldehyde ferredoxin oxidoreductase, 17fold Pyrococcus furiosus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.7.B2 58
-
 pH 8.4, 90°C Pyrococcus furiosus
1.2.7.5 additional information
-
 formaldehyde ferredoxin oxidoreductase, substrate specificity Pyrococcus furiosus
1.2.7.5 42
-
 purified formaldehyde ferredoxin oxidoreductase Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.B2 acetaldehyde + H2O + oxidized benzyl viologen
-
 Pyrococcus furiosus acetate + reduced benzyl viologen + H+
-
 ?
1.2.7.B2 formaldehyde + H2O + 2 oxidized ferredoxin formaldehyde is unlikely to be the physiologiocal substrate (Km-value: 25 mM) Pyrococcus furiosus formate + 2 reduced ferredoxin + 2 H+
-
 ?
1.2.7.B2 formaldehyde + H2O + oxidized benzyl viologen
-
 Pyrococcus furiosus formate + reduced benzyl viologen + H+
-
 ?
1.2.7.B2 glutaric dialdehyde + H2O + oxidized benzyl viologen best substrate, C5- or C6-type aldehydes might be physiologically relevant substrates Pyrococcus furiosus ? + reduced benzyl viologen + H+
-
 ?
1.2.7.B2 indole-3-acetaldehyde + H2O + oxidized benzyl viologen
-
 Pyrococcus furiosus indole-3-acetate + reduced benzyl viologen + H+
-
 ?
1.2.7.B2 additional information low activity with butyraldehyde, crotonaldehyde, no activity with: isovaleraldehyde, benzaldehyde, salicaldehyde, 2-furfuraldehyde. It does not oxidize lactate, 2-hydroxybutyrate, 2-hydroxyvalerate, 2-hydroxycaproate, or 1-ethyl-2-hydroxycaproate and it does not reduce pyruvate, 2-oxobutyrate, 2-oxovalerate, 1-ethyl-3-oxovalerate, 1-ethyl-4-oxovalerate, or 2-oxocaproate Pyrococcus furiosus ?
-
 ?
1.2.7.B2 phenylpropionaldehyde + H2O + oxidized benzyl viologen
-
 Pyrococcus furiosus phenylpropionate + reduced benzyl viologen + H+
-
 ?
1.2.7.B2 propionaldehyde + H2O + oxidized benzyl viologen
-
 Pyrococcus furiosus propionate + reduced benzyl viologen + H+
-
 ?
1.2.7.B2 succinic semialdehyde + H2O + oxidized benzyl viologen
-
 Pyrococcus furiosus succinate + reduced benzyl viologen + H+
-
 ?
1.2.7.5 acetaldehyde + H2O + oxidized benzyl viologen formaldehyde ferredoxin oxidoreductase Pyrococcus furiosus acetate + H+ + reduced benzyl viologen
-
 ir
1.2.7.5 acetaldehyde + H2O + oxidized ferredoxin formaldehyde ferredoxin oxidoreductase Pyrococcus furiosus acetate + H+ + reduced ferredoxin
-
 ir
1.2.7.5 an aldehyde + H2O + oxidized ferredoxin
-
 Pyrococcus furiosus an acid + H+ + reduced ferredoxin
-
 ?
1.2.7.5 an aldehyde + H2O + oxidized ferredoxin formaldehyde ferredoxin oxidoreductase, C1-C3 aldehydes Pyrococcus furiosus an acid + H+ + reduced ferredoxin
-
 ir
1.2.7.5 butyraldehyde + H2O + oxidized ferredoxin low activity Pyrococcus furiosus butyrate + H+ + reduced ferredoxin
-
 ir
1.2.7.5 butyraldehyde + H2O + oxidized ferredoxin formaldehyde ferredoxin oxidoreductase Pyrococcus furiosus butyrate + H+ + reduced ferredoxin
-
 ir
1.2.7.5 crotonaldehyde + H2O + oxidized benzyl viologen low activity, formaldehyde ferredoxin oxidoreductase Pyrococcus furiosus crotonate + H+ + reduced benzyl viologen
-
 ir
1.2.7.5 formaldehyde + H2O + oxidized benzyl viologen formaldehyde ferredoxin oxidoreductase Pyrococcus furiosus formate + H+ + reduced benzyl viologen
-
 ir
1.2.7.5 formaldehyde + H2O + oxidized ferredoxin formaldehyde and aldehyde ferredoxin oxidoreductases Pyrococcus furiosus formate + H+ + reduced ferredoxin
-
 ir
1.2.7.5 isovalerylaldehyde + H2O + oxidized benzyl viologen formaldehyde ferredoxin oxidoreductase, no activity Pyrococcus furiosus isovalerate + H+ + reduced benzyl viologen
-
 ?
1.2.7.5 propionaldehyde + H2O + oxidized benzyl viologen formaldehyde ferredoxin oxidoreductase Pyrococcus furiosus propionate + H+ + reduced benzyl viologen
-
 ir
1.2.7.5 propionaldehyde + H2O + oxidized ferredoxin formaldehyde ferredoxin oxidoreductase Pyrococcus furiosus propionate + H+ + reduced ferredoxin
-
 ir

Subunits

EC Number Subunits Comment Organism
1.2.7.B2 homotetramer 4 * 68700, calculated from sequence Pyrococcus furiosus
1.2.7.5 tetramer 4 * 68000, formaldehyde ferredoxin oxidoreductase, SDS-PAGE Pyrococcus furiosus

Synonyms

EC Number Synonyms Comment Organism
1.2.7.B2 FOR
-
 Pyrococcus furiosus
1.2.7.B2 formaldehyde ferredoxin oxidoreductase
-
 Pyrococcus furiosus
1.2.7.5 FOR
-
 Pyrococcus furiosus
1.2.7.5 More formaldehyde ferredoxin oxidoreductase and aldehyde ferredoxin oxidoreductase are 2 separate enzymes Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.7.5 80
-
 assay at Pyrococcus furiosus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.2.7.5 60 90 formaldehyde ferredoxin oxidoreductase, activity increases 4.5fold from 60°C to 90°C at pH 8.4 Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.7.B2 80
-
 8 h, 50% loss of activity Pyrococcus furiosus
1.2.7.5 additional information
-
 the enzyme is thermostable Pyrococcus furiosus
1.2.7.5 80
-
 formaldehyde ferredoxin oxidoreductase, t1/2: 8 h, in presence of 2 mM dithionite and 2 mM DTT, pH 8.4 Pyrococcus furiosus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.7.B2 2300
-
 Indole-3-acetaldehyde 80°C, pH 8.4 Pyrococcus furiosus
1.2.7.B2 5700
-
 Succinic semialdehyde 80°C, pH 8.4 Pyrococcus furiosus
1.2.7.B2 11000
-
 propionaldehyde 80°C, pH 8.4 Pyrococcus furiosus
1.2.7.B2 25000
-
 phenylpropionaldehyde 80°C, pH 8.4 Pyrococcus furiosus
1.2.7.B2 42000
-
 glutaric dialdehyde 80°C, pH 8.4 Pyrococcus furiosus
1.2.7.B2 44000
-
 acetaldehyde 80°C, pH 8.4 Pyrococcus furiosus
1.2.7.B2 71000
-
 formaldehyde 80°C, pH 8.4 Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.7.B2 10
-
 the activity of the pure enzyme with benzyl viologen as the electron acceptor increases linearly with pH over the pH range 5.5 (1.5 U/mg) to 10.0 (55 U/mg) at 80°C Pyrococcus furiosus
1.2.7.5 8.4
-
 assay at Pyrococcus furiosus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.7.B2 5.5 10 the activity of the pure enzyme with benzyl viologen as the electron acceptor increases linearly with pH over the pH range 5.5 (1.5 U/mg) to 10.0 (55 U/mg) at 80°C Pyrococcus furiosus
1.2.7.5 5.5 10 formaldehyde ferredoxin oxidoreductase, activity increases linearly from pH 5.5 to pH 10.0 at 80°C Pyrococcus furiosus

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.7.B2 additional information the enzyme contains a pterin cofactor Pyrococcus furiosus
1.2.7.5 Ferredoxin
-
 Pyrococcus furiosus
1.2.7.5 form A cofactor formaldehyde ferredoxin oxidoreductase Pyrococcus furiosus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.2.7.B2 90
-
 Indole-3-acetaldehyde 80°C, pH 8.4 Pyrococcus furiosus
1.2.7.B2 170
-
 propionaldehyde 80°C, pH 8.4 Pyrococcus furiosus
1.2.7.B2 710
-
 Succinic semialdehyde 80°C, pH 8.4 Pyrococcus furiosus
1.2.7.B2 730
-
 acetaldehyde 80°C, pH 8.4 Pyrococcus furiosus
1.2.7.B2 1700
-
 phenylpropionaldehyde 80°C, pH 8.4 Pyrococcus furiosus
1.2.7.B2 3000
-
 formaldehyde 80°C, pH 8.4 Pyrococcus furiosus
1.2.7.B2 60000
-
 glutaric dialdehyde 80°C, pH 8.4 Pyrococcus furiosus