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Literature summary extracted from

  • Nakamura, M.; Yamazaki, I.
    Preparation of bovine milk xanthine oxidase as a dehydrogenase form (1982), J. Biochem., 92, 1279-1286.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.17.1.4 DTT recovery from oxidase to dehydrogenase type Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.17.1.4 lipid droplet fat-globule, milk Bos taurus 5811
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.1.4 xanthine + NAD+ + H2O Bos taurus conversion of dehydrogenase to oxidase type due to oxidation of sulfhydryl groups by molecular oxygen, dehydrogenase activity recovered by treatment with dithiothreitol urate + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.17.1.4 Bos taurus
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.17.1.4 conversion of dehydrogenase to oxidase type under aerobic conditions Bos taurus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.1.4 to homogeneity, 2 types: dehydrogenase type, chromatography techniques, oxidase type: affinity chromatography Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.17.1.4 milk only oxidase type in stored milk, 94% NAD+-dependent dehydrogenase type in fresh milk Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.1.4 xanthine + NAD+ + H2O conversion of dehydrogenase to oxidase type due to oxidation of sulfhydryl groups by molecular oxygen, dehydrogenase activity recovered by treatment with dithiothreitol Bos taurus urate + NADH
-
 ?
1.17.1.4 xanthine + p-benzoquinone + H2O electron acceptor p-benzoquinone for both dehydrogenase and oxidase types Bos taurus p-benzosemiquinone + urate
-
 ?
1.17.1.4 xanthine + p-benzoquinone + H2O electron donor only for oxidase type Bos taurus hypoxanthine + hydroquinone + ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.17.1.4 2
-
 dehydrogenase type stable under anaerobic conditions Bos taurus
1.17.1.4 38
-
 dehydrogenase type unstable, change to oxidase type inhibited under anaerobic conditions Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.1.4 NAD+
-
 Bos taurus
1.17.1.4 NADH reduces xanthine oxidase to reductase activity Bos taurus