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Literature summary extracted from

  • Rajagopalan, K.V.; Handler, P.
    Purification and properties of chicken liver xanthine dehydrogenase (1967), J. Biol. Chem., 242, 4097-4107.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.1.4 ammeline competitive inhibition of xanthine oxidation Ki: 0.083 mM, uncompetitive inhibition of NADH oxidation Ki: 0.063 mM Gallus gallus
1.17.1.4 arsenite gradual inhibition of xanthine-2,6-dichloroindophenol-activity, paralleled by a corresponding increase of NADH-2,6-dichloroindophenol-activity Gallus gallus
1.17.1.4 KCN complete inhibition of xanthine dehydrogenase activity, 70% reduction of diaphorase activity Gallus gallus
1.17.1.4 methanol slight inhibition of NAD+ reduction at 1.5 M, rapid inactivation if NAD+ is replaced by 2,6-dichloroindophenol, enhanced NADH diaphorase activity Gallus gallus
1.17.1.4 NAD+ competitive inhibition of NADH oxidation, Ki: 0.0143 mM Gallus gallus
1.17.1.4 Urea competitive inhibition of xanthine oxidation Ki: 0.28 M, uncompetitive inhibition of NADH oxidation Ki: 1 M Gallus gallus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.1.4 Fe
-
 Gallus gallus
1.17.1.4 Mo
-
 Gallus gallus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.1.4 300000
-
 sedimentation equilibrium measurements Gallus gallus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.1.4 xanthine + NAD+ + H2O Gallus gallus ping-pong reaction mechanism urate + NADH
-
 r
1.17.1.4 xanthine + O2 + H2O Gallus gallus low activity urate + O2- + 2 H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.17.1.4 Gallus gallus
-
 chicken
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.1.4 to homogeneity, chromatography techniques Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.17.1.4 liver
-
 Gallus gallus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.1.4 NADH + electron acceptor + H2O NADH diaphorase activity with several acceptors Gallus gallus NAD+ + reduced electron acceptor
-
 ?
1.17.1.4 NADH + electron acceptor + H2O 2,6-dichloroindophenol, 3-acetylpyridine-adenine dinucleotide, methylene blue, phenazine methosulfate or trinitrobenzene sulfonate Gallus gallus NAD+ + reduced electron acceptor
-
 ?
1.17.1.4 xanthine + 2,6-dichlorophenolindophenol + H2O same activity compared to NAD+ as electron acceptor Gallus gallus urate + ?
-
 r
1.17.1.4 xanthine + 3-acetylpyridine-adenine dinucleotide+ + H2O same activity compared to NAD+ Gallus gallus urate + 3-acetylpyridine-adenine dinucleotide(H)
-
 r
1.17.1.4 xanthine + 3-acetylpyridine-adenine dinucleotide+ + H2O low reverse activity Gallus gallus urate + 3-acetylpyridine-adenine dinucleotide(H)
-
 r
1.17.1.4 xanthine + methylene blue + H2O
-
 Gallus gallus urate + reduced methylene blue
-
 r
1.17.1.4 xanthine + NAD+ + H2O ping-pong reaction mechanism Gallus gallus urate + NADH
-
 r
1.17.1.4 xanthine + O2 + H2O low activity Gallus gallus urate + O2- + 2 H+
-
 r
1.17.1.4 xanthine + phenazine methosulfate + H2O
-
 Gallus gallus urate + ?
-
 r
1.17.1.4 xanthine + pyridinealdehyde-NAD+ + H2O 53% of the activity compared to NAD+, low reverse activity Gallus gallus urate + pyridinealdehyde-NADH
-
 r
1.17.1.4 xanthine + thio-NAD+ + H2O same activity compared to NAD+ Gallus gallus urate + thio-NADH
-
 r
1.17.1.4 xanthine + trinitrobenzenesulfonate + H2O
-
 Gallus gallus urate + ?
-
 r

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.1.4 FAD
-
 Gallus gallus
1.17.1.4 additional information molybdoironflavoprotein: 1 : 1 : 4 ratio of molybdenum to FAD to iron Gallus gallus
1.17.1.4 NAD+
-
 Gallus gallus
1.17.1.4 NADH
-
 Gallus gallus