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Literature summary extracted from

  • Kim, J.M.; Schmid, R.D.
    Purification and characterization of a microbial xanthine dehydrogenase highly active towards hypoxanthine (1989), GBF Monogr. , Biosens. Appl. Med. , Environ. Prot. Process Control, 13, 421-424.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.1.4 0.04
-
 hypoxanthine
-
 Pseudomonas putida
1.17.1.4 0.052
-
 NAD+
-
 Pseudomonas putida
1.17.1.4 0.064
-
 xanthine
-
 Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.1.4 46000
-
 x * 92000 + x * 46000, SDS-PAGE Pseudomonas putida
1.17.1.4 92000
-
 x * 92000 + x * 46000, SDS-PAGE Pseudomonas putida
1.17.1.4 350000
-
 gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.1.4 hypoxanthine + NAD+ + H+ + O2- + H2O Pseudomonas putida more rapidly oxidized than xanthine xanthine + NADH + H2O2
-
 ?
1.17.1.4 purine + NAD+ + H2O Pseudomonas putida poor substrate ? + NADH
-
 ?
1.17.1.4 xanthine + NAD+ + H2O Pseudomonas putida
-
 urate + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.17.1.4 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.1.4 to homogeneity, chromatography techniques Pseudomonas putida

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.17.1.4 cell culture inducible by hypoxanthine Pseudomonas putida
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17.1.4 80.2
-
-
 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.1.4 hypoxanthine + NAD+ + H+ + O2- + H2O more rapidly oxidized than xanthine Pseudomonas putida xanthine + NADH + H2O2
-
 ?
1.17.1.4 purine + NAD+ + H2O poor substrate Pseudomonas putida ? + NADH
-
 ?
1.17.1.4 xanthine + NAD+ + H2O
-
 Pseudomonas putida urate + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.17.1.4 ? x * 92000 + x * 46000, SDS-PAGE Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.1.4 NAD+ cannot be replaced by NADP+ Pseudomonas putida