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Literature summary extracted from
- Wild-type and mutant forms of homoisocitric dehydrogenase in the yeast Saccharomycopsis lipolytica (1982), Eur. J. Biochem., 128, 489-494.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.1.1.87 | Mg2+ stabilizes | Saccharomycopsis lipolytica |
| 1.1.1.87 | Mn2+ stabilizes | Saccharomycopsis lipolytica |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.87 | 2-oxoadipate | - |
Saccharomycopsis lipolytica |  |
| 1.1.1.87 | homoisocitrate | - |
Saccharomycopsis lipolytica | |
| 1.1.1.87 | Oxaloglutarate | - |
Saccharomycopsis lipolytica | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.87 | K+ | required for optimal activity | Saccharomycopsis lipolytica | |
| 1.1.1.87 | Mg2+ | required | Saccharomycopsis lipolytica | |
| 1.1.1.87 | Mn2+ | stimulation of oxidative decarboxylation | Saccharomycopsis lipolytica | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.87 | 48000 | - |
native or denaturing PAGE | Saccharomycopsis lipolytica |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.87 | 1-hydroxy-1,2,4-butanetricarboxylate + NAD+ | Saccharomycopsis lipolytica | production of 2-oxoadipic acid, a precursor of lysine biosynthesis | 2-oxoadipate + CO2 + NADH | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.87 | Saccharomycopsis lipolytica | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.87 | - |
Saccharomycopsis lipolytica |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.87 | 6.304 | - |
- |
Saccharomycopsis lipolytica |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.1.1.87 | -20°C, stable as ammonium sulfate precipitate | Saccharomycopsis lipolytica |
| 1.1.1.87 | 0°C, pH 7.2, rapid loss of activity | Saccharomycopsis lipolytica |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.87 | 1-hydroxy-1,2,4-butanetricarboxylate + NAD+ | - |
Saccharomycopsis lipolytica | 2-oxoadipate + CO2 + NADH | - |
? | |
| 1.1.1.87 | 1-hydroxy-1,2,4-butanetricarboxylate + NAD+ | production of 2-oxoadipic acid, a precursor of lysine biosynthesis | Saccharomycopsis lipolytica | 2-oxoadipate + CO2 + NADH | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.1.87 | dimer | 2 * 48000, at high protein concentration or in presence of substrate the enzyme exists as dimer | Saccharomycopsis lipolytica |
| 1.1.1.87 | monomer | 1 * 48000, native or denaturing PAGE | Saccharomycopsis lipolytica |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.87 | 20 | - |
assay at | Saccharomycopsis lipolytica |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.87 | NAD+ | - |
Saccharomycopsis lipolytica | |
| 1.1.1.87 | NADH | - |
Saccharomycopsis lipolytica | |
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Release 2023.1 (January 2023)
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