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Literature summary extracted from

  • Nagel, M.; Andreesen, J.R.
    Molybdenum-dependent degradation of nicotinic acid by Bacillus sp. DSM 2923 (1989), FEMS Microbiol. Lett., 59, 147-152.
No PubMed abstract available

Protein Variants

EC Number Protein Variants Comment Organism
1.17.1.5 additional information 4 chlorate-resistant mutants are no longer able to degrade nicotinate Neobacillus niacini

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.1.5 KCN 2 mM, complete loss of enzyme activity in anaerobically incubated crude extract Neobacillus niacini

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.1.5 additional information enzyme activity increases with increasing concentrations of molybdate in growth medium Neobacillus niacini

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.1.5 nicotinate + H2O + electron acceptor Neobacillus niacini nicotinate degradation 6-hydroxynicotinate + reduced electron acceptor
-
 ?
1.17.1.5 nicotinate + H2O + electron acceptor Neobacillus niacini DSM 2923 nicotinate degradation 6-hydroxynicotinate + reduced electron acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.17.1.5 Neobacillus niacini
-
 strain AS1 isolated from the culture obtained by DSM
-
1.17.1.5 Neobacillus niacini DSM 2923
-
 strain AS1 isolated from the culture obtained by DSM
-
1.17.3.3 Bacillus sp. (in: Bacteria)
-
 DSM 2923
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17.1.5 2.2
-
 crude extract, high activity achieved if nicotinate is the only substrate Neobacillus niacini

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.1.5 nicotinate + H2O + electron acceptor high substrate specificity, mixture of phenazine ethosulfate and 2,6-dichlorophenolindophenol as electron acceptor Neobacillus niacini 6-hydroxynicotinate + reduced electron acceptor
-
 ?
1.17.1.5 nicotinate + H2O + electron acceptor nicotinate degradation Neobacillus niacini 6-hydroxynicotinate + reduced electron acceptor
-
 ?
1.17.1.5 nicotinate + H2O + electron acceptor high substrate specificity, mixture of phenazine ethosulfate and 2,6-dichlorophenolindophenol as electron acceptor Neobacillus niacini DSM 2923 6-hydroxynicotinate + reduced electron acceptor
-
 ?
1.17.1.5 nicotinate + H2O + electron acceptor nicotinate degradation Neobacillus niacini DSM 2923 6-hydroxynicotinate + reduced electron acceptor
-
 ?
1.17.3.3 6-hydroxynicotinate + H2O + O2
-
 Bacillus sp. (in: Bacteria) 2,6-dihydroxynicotinate + H2O2
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.17.1.5 30
-
 assay at Neobacillus niacini

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.1.5 molybdenum cofactor molybdoenzyme with eubacterial molybdenum cofactor Neobacillus niacini