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Literature summary extracted from

  • Hirschberg, R.; Ensign, J.C.
    Oxidation of nicotinic acid by a Bacillus species: purification and properties of nicotinic acid and 6-hydroxynicotinic acid hydroxylases (1971), J. Bacteriol., 108, 751-756.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.1.5 1,10-phenanthroline 1 mM, 30-50% inhibition Bacillus sp. (in: Bacteria)
1.17.1.5 2,2'-dipyridyl 0.1 mM, 30-50% inhibition Bacillus sp. (in: Bacteria)
1.17.1.5 Atabrine 1 mM, 30-50% inhibition Bacillus sp. (in: Bacteria)
1.17.1.5 NaCl sufficient NaCl inhibits enzyme activity significantly Bacillus sp. (in: Bacteria)

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.1.5 0.07
-
 nicotinate
-
 Bacillus sp. (in: Bacteria)

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.17.1.5 soluble
-
 Bacillus sp. (in: Bacteria)
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.1.5 400000
-
 gel filtration Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.1.5 nicotinate + H2O + electron acceptor Bacillus sp. (in: Bacteria) first step of nicotinate catabolism 6-hydroxynicotinate + reduced electron acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.17.1.5 Bacillus sp. (in: Bacteria)
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.1.5 24.4fold purification Bacillus sp. (in: Bacteria)

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.17.1.5 after denaturation by acid precipitation at pH 4.5 with HCl, recombining and neutralizing of precipitated protein and yellow supernatant results in a reconstitution of over 90% of the original activity Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17.1.5 0.83
-
-
 Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.1.5 nicotinate + H2O + electron acceptor flavin may be the primary electron acceptor, tetrazolium dye as electron acceptor Bacillus sp. (in: Bacteria) 6-hydroxynicotinate + reduced electron acceptor
-
 ?
1.17.1.5 nicotinate + H2O + electron acceptor first step of nicotinate catabolism Bacillus sp. (in: Bacteria) 6-hydroxynicotinate + reduced electron acceptor
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.17.1.5 25
-
 assay at Bacillus sp. (in: Bacteria)

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.1.5 flavin primary electron acceptor, 1.5-2 mol tightly bound flavin per mol of enzyme Bacillus sp. (in: Bacteria)
1.17.1.5 additional information approximately 8 molecules tightly bound iron per enzyme molecule Bacillus sp. (in: Bacteria)