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Literature summary extracted from

  • Debussche, L.; Thibaut, D.; Cameron, B.; Crouzet, J.; Blanche, F.
    Purification and characterization of cobyrinic acid a,c-diamide synthase from Pseudomonas denitrificans (1990), J. Bacteriol., 172, 6239-6244.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.5.9 plasmid pXL 191 is transferre from Escherichia coli MC1060 to Pseudomonas denitrificans SC510 RifT through conjugation Pseudomonas denitrificans (nom. rej.)
6.3.5.11
-
 Pseudomonas denitrificans (nom. rej.)

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.5.11 (aq)2cobyrinate substrate inhibition above 0.3 mM Pseudomonas denitrificans (nom. rej.)

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.5.9 0.00021
-
 hydrogenobyrinic acid c-monoamide pH 7.6, 30°C Pseudomonas denitrificans (nom. rej.)
6.3.5.9 0.00041
-
 Hydrogenobyrinic acid pH 7.6, 30°C Pseudomonas denitrificans (nom. rej.)
6.3.5.9 0.012
-
 ammonia pH 7.6, 30°C Pseudomonas denitrificans (nom. rej.)
6.3.5.9 0.0203
-
 glutamine pH 7.6, 30°C Pseudomonas denitrificans (nom. rej.)
6.3.5.9 0.071
-
 (CN,aq)cobyrinic acid c-monoamide pH 7.6, 30°C Pseudomonas denitrificans (nom. rej.)
6.3.5.9 0.16
-
 (CN,aq)cobyrinic acid pH 7.6, 30°C Pseudomonas denitrificans (nom. rej.)
6.3.5.9 0.25
-
 (aq)2cobyrinic acid pH 7.6, 30°C Pseudomonas denitrificans (nom. rej.)
6.3.5.11 0.00021
-
 hydrogenobyrinate c-monoamide pH 7.6, 30°C Pseudomonas denitrificans (nom. rej.)
6.3.5.11 0.00041
-
 Hydrogenobyrinate pH 7.6, 30°C Pseudomonas denitrificans (nom. rej.)
6.3.5.11 0.0203
-
 L-glutamine pH 7.6, 30°C Pseudomonas denitrificans (nom. rej.)
6.3.5.11 0.071
-
 (CN,aq)cobyrinate c-monoamide pH 7.6, 30°C Pseudomonas denitrificans (nom. rej.)
6.3.5.11 0.16
-
 (CN,aq)cobyrinate pH 7.6, 30°C Pseudomonas denitrificans (nom. rej.)
6.3.5.11 0.25
-
 (aq)2cobyrinate or above, pH 7.6, 30°C Pseudomonas denitrificans (nom. rej.)
6.3.5.11 12
-
 ammonia pH 7.6, 30°C Pseudomonas denitrificans (nom. rej.)

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.5.9 Mg2+ required Pseudomonas denitrificans (nom. rej.)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.5.9 45000
-
 2 * 45000, SDS-PAGE Pseudomonas denitrificans (nom. rej.)
6.3.5.9 86000
-
 gel filtration Pseudomonas denitrificans (nom. rej.)
6.3.5.11 46000
-
 2 * 46000, SDS-PAGE Pseudomonas denitrificans (nom. rej.)
6.3.5.11 86000
-
 gel fitlration Pseudomonas denitrificans (nom. rej.)

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.5.9 ATP + cobyrinic acid + L-glutamine + H2O Pseudomonas denitrificans (nom. rej.) cobalamin pathway from cobyrinic acid ADP + phosphate + cobyrinic acid a,c-diamide + L-glutamate
-
 ?
6.3.5.9 ATP + hydrogenobyrinic acid + L-glutamine + H2O Pseudomonas denitrificans (nom. rej.)
-
 ADP + phosphate + hydrogenobyrinic acid a,c-diamide + L-glutamate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.5.9 Pseudomonas denitrificans (nom. rej.) P21632
-
-
6.3.5.11 Pseudomonas denitrificans (nom. rej.)
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.5.9
-
 Pseudomonas denitrificans (nom. rej.)
6.3.5.11 from recombinant Pseudomonas denitrificans Pseudomonas denitrificans (nom. rej.)

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.5.11 1320
-
 pH 7.6, 30°C Pseudomonas denitrificans (nom. rej.)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.9 ATP + (aq)2cobyrinic acid + glutamine + H2O
-
 Pseudomonas denitrificans (nom. rej.) ADP + phosphate + ?
-
 ?
6.3.5.9 ATP + (CN,aq)cobyrinic acid + glutamine + H2O
-
 Pseudomonas denitrificans (nom. rej.) ADP + phosphate + (CN,aq)cobyrinic acid-c-monoamide + ?
-
 ?
6.3.5.9 ATP + (CN,aq)cobyrinic acid -monoamide + glutamine + H2O
-
 Pseudomonas denitrificans (nom. rej.) ADP + phosphate + ?
-
 ?
6.3.5.9 ATP + (CN,aq)cobyrinic acid c-monoamide + L-glutamine + H2O
-
 Pseudomonas denitrificans (nom. rej.) ADP + phosphate + ?
-
 ?
6.3.5.9 ATP + cobyrinic acid + ammonia + H2O
-
 Pseudomonas denitrificans (nom. rej.) ADP + phosphate + ?
-
 ?
6.3.5.9 ATP + cobyrinic acid + L-glutamine + H2O intermediate formation of cobyrinic acid c-monoamide Pseudomonas denitrificans (nom. rej.) ADP + phosphate + cobyrinic acid a,c-diamide + L-glutamate
-
 ?
6.3.5.9 ATP + cobyrinic acid + L-glutamine + H2O cobalamin pathway from cobyrinic acid Pseudomonas denitrificans (nom. rej.) ADP + phosphate + cobyrinic acid a,c-diamide + L-glutamate
-
 ?
6.3.5.9 ATP + hydrogenobyrinic acid + L-glutamine + H2O
-
 Pseudomonas denitrificans (nom. rej.) ADP + phosphate + hydrogenobyrinic acid a,c-diamide + L-glutamate
-
 ?
6.3.5.9 ATP + hydrogenobyrinic acid c-monoamide + L-glutamine + H2O
-
 Pseudomonas denitrificans (nom. rej.) ADP + phosphate + ?
-
 ?
6.3.5.9 additional information cobyrinic acid a,c-diamide and cobyrinic acid a,c,g-triamide are no substrates Pseudomonas denitrificans (nom. rej.) ?
-
 ?
6.3.5.11 2 ATP + (aq)2cobyrinate + 2 L-glutamine + 2 H2O 76% of the initial velocity with cobyrinate Pseudomonas denitrificans (nom. rej.) 2 ADP + 2 phosphate + (aq)2cobyrinate a,c-diamide + 2 L-glutamate
-
 ?
6.3.5.11 2 ATP + (CN,aq)cobyrinate + 2 L-glutamine + 2 H2O
-
 Pseudomonas denitrificans (nom. rej.) 2 ADP + 2 phosphate + (CN,aq)cobyrinate a,c-diamide + 2 L-glutamate
-
 ?
6.3.5.11 2 ATP + cobyrinate + 2 ammonia + 2 H2O
-
 Pseudomonas denitrificans (nom. rej.) 2 ADP + 2 phosphate + cobyrinate a,c-diamide
-
 ?
6.3.5.11 2 ATP + cobyrinate + 2 L-glutamine + 2 H2O
-
 Pseudomonas denitrificans (nom. rej.) 2 ADP + 2 phosphate + cobyrinate a,c-diamide + 2 L-glutamate
-
 ?
6.3.5.11 2 ATP + cobyrinate g-monoamide + L-glutamine + 2 H2O 145% of the initial velocity with cobyrinate Pseudomonas denitrificans (nom. rej.) 2 ADP + phosphate + cobyrinate c,g-diamide + L-glutamate product is 83% cobyrinate c,g-diamide, 17% cobyrinate a,c,g-triamide ?
6.3.5.11 2 ATP + hydrogenobyrinate + 2 L-glutamine + 2 H2O
-
 Pseudomonas denitrificans (nom. rej.) 2 ADP + 2 phosphate + hydrogenobyrinate a,c-diamide + 2 L-glutamate
-
 ?
6.3.5.11 ATP + (CN,aq)cobyrinate c-monoamide + L-glutamine + H2O
-
 Pseudomonas denitrificans (nom. rej.) ADP + phosphate + (CN,aq)cobyrinate a,c-diamide + L-glutamate
-
 ?
6.3.5.11 ATP + cobyrinate a-monoamide + L-glutamine + H2O 81% of the initial velocity with cobyrinate Pseudomonas denitrificans (nom. rej.) ADP + phosphate + cobyrinate a,c-diamide + L-glutamate product is 100% cobyrinate a,c-diamide ?
6.3.5.11 ATP + cobyrinate c-monoamide + L-glutamine + H2O 19% of the initial velocity with cobyrinate Pseudomonas denitrificans (nom. rej.) ADP + phosphate + cobyrinate a,c-diamide + L-glutamate product is 100% cobyrinate a,c-diamide ?
6.3.5.11 ATP + hydrogenobyrinate c-monoamide + L-glutamine + H2O 19% of the initial velocity with cobyrinate Pseudomonas denitrificans (nom. rej.) ADP + phosphate + hydrogenobyrinate a,c-diamide + L-glutamate
-
 ?
6.3.5.11 additional information no further amidation of cobyrinate a,c-diamide and cobyrinate a,c, g-triamide Pseudomonas denitrificans (nom. rej.) ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.5.9 dimer 2 * 45000, SDS-PAGE Pseudomonas denitrificans (nom. rej.)
6.3.5.11 dimer 2 * 46000, SDS-PAGE Pseudomonas denitrificans (nom. rej.)

Synonyms

EC Number Synonyms Comment Organism
6.3.5.9 cobyrinic acid a,c-diamide synthase
-
 Pseudomonas denitrificans (nom. rej.)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.5.9 7.3
-
 broad optimum with maximum activity from pH 6.8-8.0 Pseudomonas denitrificans (nom. rej.)
6.3.5.11 7.3
-
 broad Pseudomonas denitrificans (nom. rej.)