BRENDA - Enzyme Database

Purification and characterization of cobyrinic acid a,c-diamide synthase from Pseudomonas denitrificans

Debussche, L.; Thibaut, D.; Cameron, B.; Crouzet, J.; Blanche, F.; J. Bacteriol. 172, 6239-6244 (1990)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
6.3.5.9
plasmid pXL 191 is transferre from Escherichia coli MC1060 to Pseudomonas denitrificans SC510 RifT through conjugation
Pseudomonas denitrificans (nomen rejiciendum)
6.3.5.11
-
Pseudomonas denitrificans (nomen rejiciendum)
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
6.3.5.11
(aq)2cobyrinate
substrate inhibition above 0.3 mM
Pseudomonas denitrificans (nomen rejiciendum)
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.3.5.9
0.00021
-
hydrogenobyrinic acid c-monoamide
pH 7.6, 30°C
Pseudomonas denitrificans (nomen rejiciendum)
6.3.5.9
0.00041
-
Hydrogenobyrinic acid
pH 7.6, 30°C
Pseudomonas denitrificans (nomen rejiciendum)
6.3.5.9
0.012
-
ammonia
pH 7.6, 30°C
Pseudomonas denitrificans (nomen rejiciendum)
6.3.5.9
0.0203
-
glutamine
pH 7.6, 30°C
Pseudomonas denitrificans (nomen rejiciendum)
6.3.5.9
0.071
-
(CN,aq)cobyrinic acid c-monoamide
pH 7.6, 30°C
Pseudomonas denitrificans (nomen rejiciendum)
6.3.5.9
0.16
-
(CN,aq)cobyrinic acid
pH 7.6, 30°C
Pseudomonas denitrificans (nomen rejiciendum)
6.3.5.9
0.25
-
(aq)2cobyrinic acid
pH 7.6, 30°C
Pseudomonas denitrificans (nomen rejiciendum)
6.3.5.11
0.00021
-
hydrogenobyrinate c-monoamide
pH 7.6, 30°C
Pseudomonas denitrificans (nomen rejiciendum)
6.3.5.11
0.00041
-
Hydrogenobyrinate
pH 7.6, 30°C
Pseudomonas denitrificans (nomen rejiciendum)
6.3.5.11
0.0203
-
L-glutamine
pH 7.6, 30°C
Pseudomonas denitrificans (nomen rejiciendum)
6.3.5.11
0.071
-
(CN,aq)cobyrinate c-monoamide
pH 7.6, 30°C
Pseudomonas denitrificans (nomen rejiciendum)
6.3.5.11
0.16
-
(CN,aq)cobyrinate
pH 7.6, 30°C
Pseudomonas denitrificans (nomen rejiciendum)
6.3.5.11
0.25
-
(aq)2cobyrinate
or above, pH 7.6, 30°C
Pseudomonas denitrificans (nomen rejiciendum)
6.3.5.11
12
-
ammonia
pH 7.6, 30°C
Pseudomonas denitrificans (nomen rejiciendum)
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
6.3.5.9
Mg2+
required
Pseudomonas denitrificans (nomen rejiciendum)
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.3.5.9
45000
-
2 * 45000, SDS-PAGE
Pseudomonas denitrificans (nomen rejiciendum)
6.3.5.9
86000
-
gel filtration
Pseudomonas denitrificans (nomen rejiciendum)
6.3.5.11
46000
-
2 * 46000, SDS-PAGE
Pseudomonas denitrificans (nomen rejiciendum)
6.3.5.11
86000
-
gel fitlration
Pseudomonas denitrificans (nomen rejiciendum)
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6.3.5.9
ATP + cobyrinic acid + L-glutamine + H2O
Pseudomonas denitrificans (nomen rejiciendum)
cobalamin pathway from cobyrinic acid
ADP + phosphate + cobyrinic acid a,c-diamide + L-glutamate
-
Pseudomonas denitrificans (nomen rejiciendum)
?
6.3.5.9
ATP + hydrogenobyrinic acid + L-glutamine + H2O
Pseudomonas denitrificans (nomen rejiciendum)
-
ADP + phosphate + hydrogenobyrinic acid a,c-diamide + L-glutamate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
6.3.5.9
Pseudomonas denitrificans (nomen rejiciendum)
P21632
-
-
6.3.5.11
Pseudomonas denitrificans (nomen rejiciendum)
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
6.3.5.9
-
Pseudomonas denitrificans (nomen rejiciendum)
6.3.5.11
from recombinant Pseudomonas denitrificans
Pseudomonas denitrificans (nomen rejiciendum)
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.3.5.11
1320
-
pH 7.6, 30°C
Pseudomonas denitrificans (nomen rejiciendum)
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.3.5.9
ATP + (aq)2cobyrinic acid + glutamine + H2O
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
ADP + phosphate + ?
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
?
6.3.5.9
ATP + (CN,aq)cobyrinic acid + glutamine + H2O
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
ADP + phosphate + (CN,aq)cobyrinic acid-c-monoamide + ?
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
?
6.3.5.9
ATP + (CN,aq)cobyrinic acid -monoamide + glutamine + H2O
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
ADP + phosphate + ?
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
?
6.3.5.9
ATP + cobyrinic acid + ammonia + H2O
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
ADP + phosphate + ?
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
?
6.3.5.9
ATP + cobyrinic acid + L-glutamine + H2O
intermediate formation of cobyrinic acid c-monoamide
644524
Pseudomonas denitrificans (nomen rejiciendum)
ADP + phosphate + cobyrinic acid a,c-diamide + L-glutamate
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
?
6.3.5.9
ATP + cobyrinic acid + L-glutamine + H2O
cobalamin pathway from cobyrinic acid
644524
Pseudomonas denitrificans (nomen rejiciendum)
ADP + phosphate + cobyrinic acid a,c-diamide + L-glutamate
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
?
6.3.5.9
ATP + hydrogenobyrinic acid + L-glutamine + H2O
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
ADP + phosphate + hydrogenobyrinic acid a,c-diamide + L-glutamate
-
-
-
?
6.3.5.9
ATP + hydrogenobyrinic acid + L-glutamine + H2O
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
ADP + phosphate + hydrogenobyrinic acid a,c-diamide + L-glutamate
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
?
6.3.5.9
ATP + hydrogenobyrinic acid c-monoamide + L-glutamine + H2O
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
ADP + phosphate + ?
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
?
6.3.5.9
additional information
cobyrinic acid a,c-diamide and cobyrinic acid a,c,g-triamide are no substrates
644524
Pseudomonas denitrificans (nomen rejiciendum)
?
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
?
6.3.5.11
2 ATP + (aq)2cobyrinate + 2 L-glutamine + 2 H2O
76% of the initial velocity with cobyrinate
644524
Pseudomonas denitrificans (nomen rejiciendum)
2 ADP + 2 phosphate + (aq)2cobyrinate a,c-diamide + 2 L-glutamate
-
-
-
?
6.3.5.11
2 ATP + (CN,aq)cobyrinate + 2 L-glutamine + 2 H2O
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
2 ADP + 2 phosphate + (CN,aq)cobyrinate a,c-diamide + 2 L-glutamate
-
-
-
?
6.3.5.11
2 ATP + cobyrinate + 2 ammonia + 2 H2O
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
2 ADP + 2 phosphate + cobyrinate a,c-diamide
-
-
-
?
6.3.5.11
2 ATP + cobyrinate + 2 L-glutamine + 2 H2O
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
2 ADP + 2 phosphate + cobyrinate a,c-diamide + 2 L-glutamate
-
-
-
?
6.3.5.11
2 ATP + cobyrinate g-monoamide + L-glutamine + 2 H2O
145% of the initial velocity with cobyrinate
644524
Pseudomonas denitrificans (nomen rejiciendum)
2 ADP + phosphate + cobyrinate c,g-diamide + L-glutamate
product is 83% cobyrinate c,g-diamide, 17% cobyrinate a,c,g-triamide
-
-
?
6.3.5.11
2 ATP + hydrogenobyrinate + 2 L-glutamine + 2 H2O
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
2 ADP + 2 phosphate + hydrogenobyrinate a,c-diamide + 2 L-glutamate
-
-
-
?
6.3.5.11
ATP + (CN,aq)cobyrinate c-monoamide + L-glutamine + H2O
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
ADP + phosphate + (CN,aq)cobyrinate a,c-diamide + L-glutamate
-
-
-
?
6.3.5.11
ATP + cobyrinate a-monoamide + L-glutamine + H2O
81% of the initial velocity with cobyrinate
644524
Pseudomonas denitrificans (nomen rejiciendum)
ADP + phosphate + cobyrinate a,c-diamide + L-glutamate
product is 100% cobyrinate a,c-diamide
-
-
?
6.3.5.11
ATP + cobyrinate c-monoamide + L-glutamine + H2O
19% of the initial velocity with cobyrinate
644524
Pseudomonas denitrificans (nomen rejiciendum)
ADP + phosphate + cobyrinate a,c-diamide + L-glutamate
product is 100% cobyrinate a,c-diamide
-
-
?
6.3.5.11
ATP + hydrogenobyrinate c-monoamide + L-glutamine + H2O
19% of the initial velocity with cobyrinate
644524
Pseudomonas denitrificans (nomen rejiciendum)
ADP + phosphate + hydrogenobyrinate a,c-diamide + L-glutamate
-
-
-
?
6.3.5.11
additional information
no further amidation of cobyrinate a,c-diamide and cobyrinate a,c, g-triamide
644524
Pseudomonas denitrificans (nomen rejiciendum)
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
6.3.5.9
dimer
2 * 45000, SDS-PAGE
Pseudomonas denitrificans (nomen rejiciendum)
6.3.5.11
dimer
2 * 46000, SDS-PAGE
Pseudomonas denitrificans (nomen rejiciendum)
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.3.5.9
7.3
-
broad optimum with maximum activity from pH 6.8-8.0
Pseudomonas denitrificans (nomen rejiciendum)
6.3.5.11
7.3
-
broad
Pseudomonas denitrificans (nomen rejiciendum)
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
6.3.5.9
plasmid pXL 191 is transferre from Escherichia coli MC1060 to Pseudomonas denitrificans SC510 RifT through conjugation
Pseudomonas denitrificans (nomen rejiciendum)
6.3.5.11
-
Pseudomonas denitrificans (nomen rejiciendum)
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
6.3.5.11
(aq)2cobyrinate
substrate inhibition above 0.3 mM
Pseudomonas denitrificans (nomen rejiciendum)
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.3.5.9
0.00021
-
hydrogenobyrinic acid c-monoamide
pH 7.6, 30°C
Pseudomonas denitrificans (nomen rejiciendum)
6.3.5.9
0.00041
-
Hydrogenobyrinic acid
pH 7.6, 30°C
Pseudomonas denitrificans (nomen rejiciendum)
6.3.5.9
0.012
-
ammonia
pH 7.6, 30°C
Pseudomonas denitrificans (nomen rejiciendum)
6.3.5.9
0.0203
-
glutamine
pH 7.6, 30°C
Pseudomonas denitrificans (nomen rejiciendum)
6.3.5.9
0.071
-
(CN,aq)cobyrinic acid c-monoamide
pH 7.6, 30°C
Pseudomonas denitrificans (nomen rejiciendum)
6.3.5.9
0.16
-
(CN,aq)cobyrinic acid
pH 7.6, 30°C
Pseudomonas denitrificans (nomen rejiciendum)
6.3.5.9
0.25
-
(aq)2cobyrinic acid
pH 7.6, 30°C
Pseudomonas denitrificans (nomen rejiciendum)
6.3.5.11
0.00021
-
hydrogenobyrinate c-monoamide
pH 7.6, 30°C
Pseudomonas denitrificans (nomen rejiciendum)
6.3.5.11
0.00041
-
Hydrogenobyrinate
pH 7.6, 30°C
Pseudomonas denitrificans (nomen rejiciendum)
6.3.5.11
0.0203
-
L-glutamine
pH 7.6, 30°C
Pseudomonas denitrificans (nomen rejiciendum)
6.3.5.11
0.071
-
(CN,aq)cobyrinate c-monoamide
pH 7.6, 30°C
Pseudomonas denitrificans (nomen rejiciendum)
6.3.5.11
0.16
-
(CN,aq)cobyrinate
pH 7.6, 30°C
Pseudomonas denitrificans (nomen rejiciendum)
6.3.5.11
0.25
-
(aq)2cobyrinate
or above, pH 7.6, 30°C
Pseudomonas denitrificans (nomen rejiciendum)
6.3.5.11
12
-
ammonia
pH 7.6, 30°C
Pseudomonas denitrificans (nomen rejiciendum)
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
6.3.5.9
Mg2+
required
Pseudomonas denitrificans (nomen rejiciendum)
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.3.5.9
45000
-
2 * 45000, SDS-PAGE
Pseudomonas denitrificans (nomen rejiciendum)
6.3.5.9
86000
-
gel filtration
Pseudomonas denitrificans (nomen rejiciendum)
6.3.5.11
46000
-
2 * 46000, SDS-PAGE
Pseudomonas denitrificans (nomen rejiciendum)
6.3.5.11
86000
-
gel fitlration
Pseudomonas denitrificans (nomen rejiciendum)
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6.3.5.9
ATP + cobyrinic acid + L-glutamine + H2O
Pseudomonas denitrificans (nomen rejiciendum)
cobalamin pathway from cobyrinic acid
ADP + phosphate + cobyrinic acid a,c-diamide + L-glutamate
-
Pseudomonas denitrificans (nomen rejiciendum)
?
6.3.5.9
ATP + hydrogenobyrinic acid + L-glutamine + H2O
Pseudomonas denitrificans (nomen rejiciendum)
-
ADP + phosphate + hydrogenobyrinic acid a,c-diamide + L-glutamate
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
6.3.5.9
-
Pseudomonas denitrificans (nomen rejiciendum)
6.3.5.11
from recombinant Pseudomonas denitrificans
Pseudomonas denitrificans (nomen rejiciendum)
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.3.5.11
1320
-
pH 7.6, 30°C
Pseudomonas denitrificans (nomen rejiciendum)
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.3.5.9
ATP + (aq)2cobyrinic acid + glutamine + H2O
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
ADP + phosphate + ?
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
?
6.3.5.9
ATP + (CN,aq)cobyrinic acid + glutamine + H2O
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
ADP + phosphate + (CN,aq)cobyrinic acid-c-monoamide + ?
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
?
6.3.5.9
ATP + (CN,aq)cobyrinic acid -monoamide + glutamine + H2O
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
ADP + phosphate + ?
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
?
6.3.5.9
ATP + cobyrinic acid + ammonia + H2O
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
ADP + phosphate + ?
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
?
6.3.5.9
ATP + cobyrinic acid + L-glutamine + H2O
intermediate formation of cobyrinic acid c-monoamide
644524
Pseudomonas denitrificans (nomen rejiciendum)
ADP + phosphate + cobyrinic acid a,c-diamide + L-glutamate
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
?
6.3.5.9
ATP + cobyrinic acid + L-glutamine + H2O
cobalamin pathway from cobyrinic acid
644524
Pseudomonas denitrificans (nomen rejiciendum)
ADP + phosphate + cobyrinic acid a,c-diamide + L-glutamate
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
?
6.3.5.9
ATP + hydrogenobyrinic acid + L-glutamine + H2O
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
ADP + phosphate + hydrogenobyrinic acid a,c-diamide + L-glutamate
-
-
-
?
6.3.5.9
ATP + hydrogenobyrinic acid + L-glutamine + H2O
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
ADP + phosphate + hydrogenobyrinic acid a,c-diamide + L-glutamate
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
?
6.3.5.9
ATP + hydrogenobyrinic acid c-monoamide + L-glutamine + H2O
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
ADP + phosphate + ?
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
?
6.3.5.9
additional information
cobyrinic acid a,c-diamide and cobyrinic acid a,c,g-triamide are no substrates
644524
Pseudomonas denitrificans (nomen rejiciendum)
?
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
?
6.3.5.11
2 ATP + (aq)2cobyrinate + 2 L-glutamine + 2 H2O
76% of the initial velocity with cobyrinate
644524
Pseudomonas denitrificans (nomen rejiciendum)
2 ADP + 2 phosphate + (aq)2cobyrinate a,c-diamide + 2 L-glutamate
-
-
-
?
6.3.5.11
2 ATP + (CN,aq)cobyrinate + 2 L-glutamine + 2 H2O
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
2 ADP + 2 phosphate + (CN,aq)cobyrinate a,c-diamide + 2 L-glutamate
-
-
-
?
6.3.5.11
2 ATP + cobyrinate + 2 ammonia + 2 H2O
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
2 ADP + 2 phosphate + cobyrinate a,c-diamide
-
-
-
?
6.3.5.11
2 ATP + cobyrinate + 2 L-glutamine + 2 H2O
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
2 ADP + 2 phosphate + cobyrinate a,c-diamide + 2 L-glutamate
-
-
-
?
6.3.5.11
2 ATP + cobyrinate g-monoamide + L-glutamine + 2 H2O
145% of the initial velocity with cobyrinate
644524
Pseudomonas denitrificans (nomen rejiciendum)
2 ADP + phosphate + cobyrinate c,g-diamide + L-glutamate
product is 83% cobyrinate c,g-diamide, 17% cobyrinate a,c,g-triamide
-
-
?
6.3.5.11
2 ATP + hydrogenobyrinate + 2 L-glutamine + 2 H2O
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
2 ADP + 2 phosphate + hydrogenobyrinate a,c-diamide + 2 L-glutamate
-
-
-
?
6.3.5.11
ATP + (CN,aq)cobyrinate c-monoamide + L-glutamine + H2O
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
ADP + phosphate + (CN,aq)cobyrinate a,c-diamide + L-glutamate
-
-
-
?
6.3.5.11
ATP + cobyrinate a-monoamide + L-glutamine + H2O
81% of the initial velocity with cobyrinate
644524
Pseudomonas denitrificans (nomen rejiciendum)
ADP + phosphate + cobyrinate a,c-diamide + L-glutamate
product is 100% cobyrinate a,c-diamide
-
-
?
6.3.5.11
ATP + cobyrinate c-monoamide + L-glutamine + H2O
19% of the initial velocity with cobyrinate
644524
Pseudomonas denitrificans (nomen rejiciendum)
ADP + phosphate + cobyrinate a,c-diamide + L-glutamate
product is 100% cobyrinate a,c-diamide
-
-
?
6.3.5.11
ATP + hydrogenobyrinate c-monoamide + L-glutamine + H2O
19% of the initial velocity with cobyrinate
644524
Pseudomonas denitrificans (nomen rejiciendum)
ADP + phosphate + hydrogenobyrinate a,c-diamide + L-glutamate
-
-
-
?
6.3.5.11
additional information
no further amidation of cobyrinate a,c-diamide and cobyrinate a,c, g-triamide
644524
Pseudomonas denitrificans (nomen rejiciendum)
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
6.3.5.9
dimer
2 * 45000, SDS-PAGE
Pseudomonas denitrificans (nomen rejiciendum)
6.3.5.11
dimer
2 * 46000, SDS-PAGE
Pseudomonas denitrificans (nomen rejiciendum)
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.3.5.9
7.3
-
broad optimum with maximum activity from pH 6.8-8.0
Pseudomonas denitrificans (nomen rejiciendum)
6.3.5.11
7.3
-
broad
Pseudomonas denitrificans (nomen rejiciendum)