BRENDA - Enzyme Database show

Solubilization and characterization of the leukotriene C4 synthetase of rat basophil leukemia cells: a novel, particulate glutathione S-transferase

Bach, M.K.; Brashler, J.R.; Morton, D.R.; Arch. Biochem. Biophys. 230, 455-465 (1984)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
4.4.1.20
17-beta-estradiol-3-sulfate
stimulates
Rattus norvegicus
4.4.1.20
17-beta-estradiol-3-sulfate-17 glucuronide
stimulates
Rattus norvegicus
4.4.1.20
estriol-3-sulfate
stimulates
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.4.1.20
estrone-3-sulfate
IC50: 1.9 mM
Rattus norvegicus
4.4.1.20
p-aminohippuric acid
IC50: 0.26 mM
Rattus norvegicus
4.4.1.20
probenecid
IC50: 17 mM
Rattus norvegicus
4.4.1.20
Sulfobromophthalein
IC50: 0.06 mM
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.4.1.20
0.21
-
leukotriene A4
pH 6.5, 30°C
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.4.1.20
particle-bound
-
Rattus norvegicus
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.4.1.20
Rattus norvegicus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.4.1.20
basophilic leukemia cell line
-
Rattus norvegicus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.4.1.20
leukotriene A4 + glutathione
-
644509
Rattus norvegicus
leukotriene C4 + H2O
-
644509
Rattus norvegicus
?
IC50 Value
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
4.4.1.20
0.06
-
IC50: 0.06 mM
Rattus norvegicus
Sulfobromophthalein
4.4.1.20
0.26
-
IC50: 0.26 mM
Rattus norvegicus
p-aminohippuric acid
4.4.1.20
1.9
-
IC50: 1.9 mM
Rattus norvegicus
estrone-3-sulfate
4.4.1.20
17
-
IC50: 17 mM
Rattus norvegicus
probenecid
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
4.4.1.20
17-beta-estradiol-3-sulfate
stimulates
Rattus norvegicus
4.4.1.20
17-beta-estradiol-3-sulfate-17 glucuronide
stimulates
Rattus norvegicus
4.4.1.20
estriol-3-sulfate
stimulates
Rattus norvegicus
IC50 Value (protein specific)
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
4.4.1.20
0.06
-
IC50: 0.06 mM
Rattus norvegicus
Sulfobromophthalein
4.4.1.20
0.26
-
IC50: 0.26 mM
Rattus norvegicus
p-aminohippuric acid
4.4.1.20
1.9
-
IC50: 1.9 mM
Rattus norvegicus
estrone-3-sulfate
4.4.1.20
17
-
IC50: 17 mM
Rattus norvegicus
probenecid
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.4.1.20
estrone-3-sulfate
IC50: 1.9 mM
Rattus norvegicus
4.4.1.20
p-aminohippuric acid
IC50: 0.26 mM
Rattus norvegicus
4.4.1.20
probenecid
IC50: 17 mM
Rattus norvegicus
4.4.1.20
Sulfobromophthalein
IC50: 0.06 mM
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.4.1.20
0.21
-
leukotriene A4
pH 6.5, 30°C
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.4.1.20
particle-bound
-
Rattus norvegicus
-
-
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.4.1.20
basophilic leukemia cell line
-
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.4.1.20
leukotriene A4 + glutathione
-
644509
Rattus norvegicus
leukotriene C4 + H2O
-
644509
Rattus norvegicus
?