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Literature summary extracted from

  • Soederstroem, M.; Morgenstern, R.; Hammarstroem, S.
    Protein-protein interaction affinity chromatography of leukotriene C4 synthase (1995), Protein Expr. Purif., 6, 352-356.
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.20 Homo sapiens
-
-
-
4.4.1.20 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.20 affinity chromatography purification based on specific interaction between leukotriene C4 synthase and microsomal glutathione S-transferase which ocurs in the presence of magnesium ion Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.4.1.20 0.729
-
-
Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.20 leukotriene A4 + glutathione
-
Mus musculus leukotriene C4 + H2O
-
?
4.4.1.20 leukotriene A4 + glutathione
-
Homo sapiens leukotriene C4 + H2O
-
?

Subunits

EC Number Subunits Comment Organism
4.4.1.20 More the major components migrate as 18000 Da, 37000 Da, 48000 Da and 60000 Da proteins in SDS-PAGE Homo sapiens