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Literature summary extracted from

  • Yokota, A.; Enomoto, K.; Tomita, F.
    Purification and properties of inulin fructotransferase (depolymerizing) from Arhrobacter sp. H65-7 (1991), J. Ferment. Bioeng., 72, 262-265.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.2.18 0.8
-
inulin with MW of inulin taken as 5000 Da Arthrobacter sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.2.18 extracellular
-
Arthrobacter sp.
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.18 49000
-
2 * 49000, SDS-PAGE Arthrobacter sp.
4.2.2.18 100000
-
gel filtration Arthrobacter sp.

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.18 Arthrobacter sp.
-
gene ift
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.18 from strain H65-7 Arthrobacter sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.2.18 culture medium
-
Arthrobacter sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.2.18 604
-
purified enzyme Arthrobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.18 inulin other substrates: sucrose, 1-kestose, nystose, 1-F-fructofuranosyl-nystose, raffinose, melibiose, melezitose, stachyose, bacterial levans Arthrobacter sp. 3 di-D-fructofuranose 1,2':2,3' dianhydride + ? after exhaustive digestion of inulin nystose and 1-F-fructofuranosyl-nystose are produced in addition to di-D-fructofuranose 1,2':2,3' dianhydride ?
4.2.2.18 inulin other substrates: sucrose, 1-kestose, nystose, 1-F-fructofuranosyl-nystose, raffinose, melibiose, melezitose, stachyose, bacterial levans Arthrobacter sp. 3 di-D-fructofuranose 1,2':2,3' dianhydride + ? + oligosaccharides, small amount ?

Subunits

EC Number Subunits Comment Organism
4.2.2.18 dimer 2 * 49000, SDS-PAGE Arthrobacter sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.18 60
-
-
Arthrobacter sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.2.18 40
-
about 50% of activity maximum at 40°C and 75°C Arthrobacter sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.18 70
-
stable up to Arthrobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.18 additional information
-
strain H65-7, pI: 4.7 Arthrobacter sp.
4.2.2.18 5.5
-
-
Arthrobacter sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.2.18 4 8 about 50% of activity maximum at pH 4.0 and pH 8.0 Arthrobacter sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.2.18 4.5
-
stable Arthrobacter sp.