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Literature summary extracted from

  • Kang, S.I.; Kim, W.P.; Chang, Y.J.; Kim, S.I.
    Purification and properties of inulin fructotransferase (DFA III-producing) from Bacillus sp. snu-7 (1998), Biosci. Biotechnol. Biochem., 62, 628-631.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.18 Ba2+ slight inhibition Bacillus sp. (in: Bacteria)
4.2.2.18 Cu2+ strong inhibition at 1 mM Bacillus sp. (in: Bacteria)
4.2.2.18 Fe2+ strong inhibition at 1 mM Bacillus sp. (in: Bacteria)
4.2.2.18 Hg2+ complete inhibition at 1 mM Bacillus sp. (in: Bacteria)
4.2.2.18 additional information no effect of EDTA and cysteine Bacillus sp. (in: Bacteria)
4.2.2.18 Ni2+ slight inhibition Bacillus sp. (in: Bacteria)

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.2.18 5.4
-
 inulin with MW of inulin taken as 5000 Da Bacillus sp. (in: Bacteria)

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.2.18 extracellular
-
 Bacillus sp. (in: Bacteria)
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.18 additional information no effect of Mg2+ and Co2+ Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.18 additional information
-
 amino acid composition Bacillus sp. (in: Bacteria)
4.2.2.18 additional information
-
 N-terminal amino acid sequence Bacillus sp. (in: Bacteria)
4.2.2.18 62000
-
 x * 62000, SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.18 Bacillus sp. (in: Bacteria)
-
-
-
4.2.2.18 Bacillus sp. (in: Bacteria) snu-7
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.18
-
 Bacillus sp. (in: Bacteria)

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.2.18 culture medium
-
 Bacillus sp. (in: Bacteria)
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.2.18 45.8
-
 purified enzyme Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.18 inulin
-
 Bacillus sp. (in: Bacteria) 3 di-D-fructofuranose 1,2':2,3' dianhydride + ? i.e. DFA III ?
4.2.2.18 inulin
-
 Bacillus sp. (in: Bacteria) 3 di-D-fructofuranose 1,2':2,3' dianhydride + ? other products through exhaustive enzymic digestion of inulin: 1-kestose, 1-nystose, and 1-F-fructofuranosyl-nystose ?
4.2.2.18 inulin
-
 Bacillus sp. (in: Bacteria) snu-7 3 di-D-fructofuranose 1,2':2,3' dianhydride + ? i.e. DFA III ?
4.2.2.18 inulin
-
 Bacillus sp. (in: Bacteria) snu-7 3 di-D-fructofuranose 1,2':2,3' dianhydride + ? other products through exhaustive enzymic digestion of inulin: 1-kestose, 1-nystose, and 1-F-fructofuranosyl-nystose ?

Subunits

EC Number Subunits Comment Organism
4.2.2.18 ? x * 62000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.18 40
-
-
 Bacillus sp. (in: Bacteria)

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.2.18 30
-
 80% of maximal activity at 30°C and 60°C Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.18 60
-
 stable up to Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.18 6
-
-
 Bacillus sp. (in: Bacteria)

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.2.18 4 9
-
 Bacillus sp. (in: Bacteria)

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.2.18 4
-
 80% of maximal activity at pH 4.0 and pH 7.0 Bacillus sp. (in: Bacteria)