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Literature summary extracted from
- An efficient purification with a high recovery of the inulin fructotransferase of Arthrobacter sp. A-6 from recombinant Escherichia coli (2000), Biotechnol. Lett., 22, 291-293.
No PubMed abstract available
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.2.2.18 | strain A-6, functional expression in Escherichia coli DH5alpha/pDFE | Arthrobacter sp. |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.18 | 46500 | - |
x * 46500, SDS-PAGE | Arthrobacter sp. |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.2.18 | Arthrobacter sp. | Q9FA01 | - |
- |
| 4.2.2.18 | Arthrobacter sp. A-6 | Q9FA01 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.2.18 | enzyme from strain A-6 recombinant in Escherichia coli | Arthrobacter sp. |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.18 | 740 | - |
purified recombinant enzyme | Arthrobacter sp. |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.2.18 | inulin | - |
Arthrobacter sp. | 3 di-D-fructofuranose 1,2':2,3' dianhydride + ? | i.e. DFA III | ? |  |
| 4.2.2.18 | inulin | - |
Arthrobacter sp. A-6 | 3 di-D-fructofuranose 1,2':2,3' dianhydride + ? | i.e. DFA III | ? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.2.2.18 | ? | x * 46500, SDS-PAGE | Arthrobacter sp. |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.2.2.18 | IFTase | - |
Arthrobacter sp. |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.18 | 70 | - |
recombinant enzyme | Arthrobacter sp. |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.18 | 70 | - |
recombinant enzyme, stable for 5 h | Arthrobacter sp. |
| 4.2.2.18 | 80 | - |
recombinant enzyme, 65% remaining activity after 30 min | Arthrobacter sp. |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.18 | 6 | - |
assay at | Arthrobacter sp. |
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Release 2023.1 (January 2023)
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