EC Number | Application | Comment | Organism |
---|---|---|---|
4.2.2.17 | nutrition | di-D-fructofuranose-1,2':2,1'-dianhydride is expected to be useful as a low-calorie sweetener | Arthrobacter |
4.2.2.18 | biotechnology | improved enzyme for large scale production of low-calorie sweet food additive | Arthrobacter sp. |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
4.2.2.18 | extracellular | - |
Arthrobacter sp. | - |
- |
4.2.2.18 | extracellular | - |
Streptomyces sp. | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.2.2.17 | 40000 | - |
SDS-PAGE and gel filtration | Arthrobacter |
4.2.2.18 | 36000 | - |
2 * 36000, SDS-PAGE | Streptomyces sp. |
4.2.2.18 | 40000 | - |
gel filtration | Arthrobacter sp. |
4.2.2.18 | 46000 | - |
gel filtration | Arthrobacter globiformis |
4.2.2.18 | 70000 | - |
gel filtration | Streptomyces sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.2.17 | inulin | Arthrobacter | - |
3 di-D-fructofuranose-1,2':2,1'-dianhydride + ? | i.e. DFA I | ? | |
4.2.2.17 | inulin | Arthrobacter MC12493 | - |
3 di-D-fructofuranose-1,2':2,1'-dianhydride + ? | i.e. DFA I | ? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.2.17 | Arthrobacter | - |
MC12493 | - |
4.2.2.17 | Arthrobacter MC12493 | - |
MC12493 | - |
4.2.2.18 | Arthrobacter globiformis | - |
- |
- |
4.2.2.18 | Arthrobacter globiformis S14-3 | - |
- |
- |
4.2.2.18 | Arthrobacter sp. | - |
- |
- |
4.2.2.18 | Arthrobacter sp. MCI-2493 | - |
- |
- |
4.2.2.18 | Streptomyces sp. | - |
- |
- |
4.2.2.18 | Streptomyces sp. MCI-2524 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.2.18 | - |
Arthrobacter globiformis |
4.2.2.18 | - |
Streptomyces sp. |
4.2.2.18 | from strain MCI-2493 | Arthrobacter sp. |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
4.2.2.18 | culture medium | - |
Arthrobacter sp. | - |
4.2.2.18 | culture medium | - |
Streptomyces sp. | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.2.2.18 | 81.5 | - |
purified enzyme | Arthrobacter globiformis |
4.2.2.18 | 256 | - |
purified enzyme | Streptomyces sp. |
4.2.2.18 | 384 | - |
purified enzyme | Arthrobacter sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.2.17 | inulin | - |
Arthrobacter | 3 di-D-fructofuranose-1,2':2,1'-dianhydride + ? | i.e. DFA I | ? | |
4.2.2.17 | inulin | - |
Arthrobacter MC12493 | 3 di-D-fructofuranose-1,2':2,1'-dianhydride + ? | i.e. DFA I | ? | |
4.2.2.18 | inulin | cf. EC 2.4.1.200 | Arthrobacter globiformis | di-D-fructofuranose 1,2':2,1' dianhydride + ? | i.e. DFA I | ? | |
4.2.2.18 | inulin | cf. EC 2.4.1.200 | Arthrobacter sp. | di-D-fructofuranose 1,2':2,1' dianhydride + ? | i.e. DFA I | ? | |
4.2.2.18 | inulin | cf. EC 2.4.1.200 | Streptomyces sp. | di-D-fructofuranose 1,2':2,1' dianhydride + ? | i.e. DFA I | ? | |
4.2.2.18 | inulin | cf. EC 2.4.1.200 | Arthrobacter sp. | di-D-fructofuranose 1,2':2,1' dianhydride + ? | other products are: nystose and 1-F-fructofuranosyl-nystose | ? | |
4.2.2.18 | inulin | cf. EC 2.4.1.200 | Arthrobacter sp. MCI-2493 | di-D-fructofuranose 1,2':2,1' dianhydride + ? | i.e. DFA I | ? | |
4.2.2.18 | inulin | cf. EC 2.4.1.200 | Arthrobacter sp. MCI-2493 | di-D-fructofuranose 1,2':2,1' dianhydride + ? | other products are: nystose and 1-F-fructofuranosyl-nystose | ? | |
4.2.2.18 | inulin | cf. EC 2.4.1.200 | Streptomyces sp. MCI-2524 | di-D-fructofuranose 1,2':2,1' dianhydride + ? | i.e. DFA I | ? | |
4.2.2.18 | inulin | cf. EC 2.4.1.200 | Arthrobacter globiformis S14-3 | di-D-fructofuranose 1,2':2,1' dianhydride + ? | i.e. DFA I | ? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.2.2.17 | monomer | 1 * 40000, gel filtration | Arthrobacter |
4.2.2.18 | dimer | - |
Arthrobacter sp. |
4.2.2.18 | dimer | 2 * 36000, SDS-PAGE | Streptomyces sp. |
4.2.2.18 | monomer | 1 * 40000, SDS-PAGE | Arthrobacter sp. |
4.2.2.18 | monomer | 1 * 46000, SDS-PAGE | Arthrobacter globiformis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.2.17 | 50 | - |
- |
Arthrobacter |
4.2.2.18 | 40 | - |
- |
Arthrobacter globiformis |
4.2.2.18 | 50 | - |
- |
Arthrobacter sp. |
4.2.2.18 | 65 | - |
- |
Streptomyces sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.2.17 | 70 | - |
pH 6, 30 min., 80% activity | Arthrobacter |
4.2.2.17 | 80 | - |
30 min., complete activity loss | Arthrobacter |
4.2.2.18 | 70 | - |
more than 80% activity remains after 30 min, pH 6.0 | Arthrobacter sp. |
4.2.2.18 | 80 | - |
- |
Arthrobacter globiformis |
4.2.2.18 | 80 | - |
most of the activity is lost after 30 min | Arthrobacter sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.2.2.17 | 6 | - |
- |
Arthrobacter |
4.2.2.18 | 6 | - |
- |
Arthrobacter globiformis |
4.2.2.18 | 6 | - |
- |
Arthrobacter sp. |
4.2.2.18 | 6 | - |
- |
Streptomyces sp. |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
4.2.2.17 | 4 | - |
30°C, 30 min., stable | Arthrobacter |
4.2.2.18 | 4 | - |
- |
Arthrobacter globiformis |
4.2.2.18 | 4 | - |
30°C, 30 min, stable | Arthrobacter sp. |