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Literature summary extracted from

  • Song, K.B.; Bae, K.S.; Lee, Y.B.; Lee, K.Y.; Rhee, S.K.
    Characteristics of levan fructotransferase from Arthrobacter ureafaciens K2032 and difructose anhydride IV formation from levan (2000), Enzyme Microb. Technol., 27, 212-218.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.16 FeCl2 1 mM, 96% inhibition Paenarthrobacter ureafaciens
4.2.2.16 HgCl2 1 mM, complete inhibition Paenarthrobacter ureafaciens
4.2.2.16 KCl 1 mM, 11% inhibition Paenarthrobacter ureafaciens
4.2.2.16 LiCl 1 mM, 8% inhibition Paenarthrobacter ureafaciens
4.2.2.16 MgCl2 1 mM, 37% inhibition Paenarthrobacter ureafaciens
4.2.2.16 MnCl2 1 mM, 91% inhibition Paenarthrobacter ureafaciens
4.2.2.16 NH4Cl 1 mM, 54% inhibition Paenarthrobacter ureafaciens
4.2.2.16 NiCl2 1 mM, 26% inhibition Paenarthrobacter ureafaciens
4.2.2.16 SDS 10 mM, 68% inhibition Paenarthrobacter ureafaciens
4.2.2.16 ZnCl2 1 mM, 76% inhibition Paenarthrobacter ureafaciens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.2.16 extracellular
-
 Paenarthrobacter ureafaciens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.16 CaCl2 1 mM, activation to 156% of control. 10 mM, activation to 181% of control Paenarthrobacter ureafaciens
4.2.2.16 NaCl 1 mM, activation to 125% of control. 10 mM, activation to 142% of control Paenarthrobacter ureafaciens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.16 51000
-
 2 * 51000, SDS-PAGE Paenarthrobacter ureafaciens
4.2.2.16 96000
-
 gel filtration Paenarthrobacter ureafaciens

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.16 Paenarthrobacter ureafaciens
-
-
-
4.2.2.16 Paenarthrobacter ureafaciens K2032
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.16
-
 Paenarthrobacter ureafaciens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.2.16 culture medium
-
 Paenarthrobacter ureafaciens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.2.16 2269
-
-
 Paenarthrobacter ureafaciens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.16 levan
-
 Paenarthrobacter ureafaciens di-D-fructofuranose-2,6':2',6 dianhydride + ? and small amounts of several oligosaccharides and free fructose ?
4.2.2.16 levan
-
 Paenarthrobacter ureafaciens K2032 di-D-fructofuranose-2,6':2',6 dianhydride + ? and small amounts of several oligosaccharides and free fructose ?

Subunits

EC Number Subunits Comment Organism
4.2.2.16 dimer 2 * 51000, SDS-PAGE Paenarthrobacter ureafaciens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.16 additional information
-
-
 Paenarthrobacter ureafaciens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.16 50
-
 30 min, enzyme retains 87% of its activity Paenarthrobacter ureafaciens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.16 5.8
-
-
 Paenarthrobacter ureafaciens