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Literature summary extracted from

  • Thomas, M.G.; Thompson, T.B.; Rayment, I.; Escalante-Semerena, J.C.
    Analysis of the adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase (CobU) enzyme of Salmonella typhimurium LT2. Identification of residue His-46 as the site of guanylylation (2000), J. Biol. Chem., 275, 27576-27586.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.156 H45A site-directed mutagenesis Salmonella enterica subsp. enterica serovar Typhimurium
2.7.1.156 H45A/H46A site-directed mutagenesis Salmonella enterica subsp. enterica serovar Typhimurium
2.7.1.156 H46A site-directed mutagenesis Salmonella enterica subsp. enterica serovar Typhimurium
2.7.1.156 H46N site-directed mutagenesis Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.62 H45A site-directed mutagenesis Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.62 H45A/H46A site-directed mutagenesis Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.62 H46A site-directed mutagenesis Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.62 H46N site-directed mutagenesis Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.156 adenosylcobinamide-phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.1.156 diphosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.156 ATP + adenosylcobinamide Salmonella enterica subsp. enterica serovar Typhimurium
-
 adenosylcobinamide phosphate + ADP
-
 ?
2.7.1.156 ATP + adenosylcobinamide Salmonella enterica subsp. enterica serovar Typhimurium late steps of cobalamin biosynthesis, involved in de novo synthesis of vitamin B12, nucleotide loop assembly, enzyme functions only if Salmonella typhimurium is assimilating cobinamide from its environment, CobU becomes less active during the kinase reaction due to the formation of a covalent CobU-AMP complex that holds CobU in an altered conformation adenosylcobinamide phosphate + ADP
-
 ?
2.7.1.156 GTP + adenosylcobinamide Salmonella enterica subsp. enterica serovar Typhimurium
-
 adenosylcobinamide phosphate + GDP
-
 ?
2.7.7.62 GTP + adenosylcobinamide phosphate Salmonella enterica subsp. enterica serovar Typhimurium
-
 diphosphate + adenosylcobinamide-GDP late steps of cobalamin biosynthesis, involved in de novo synthesis of vitamin B12, nucleotide loop assembly, required for assimilation of exogenous cobinamide and de novo synthesis of adenosylcobalamin, reaction proceeds via a covalently modified CobU-GMP intermediate ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.156 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-
2.7.7.62 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.156
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.62
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.156 0.00000003
-
 GTP:kinase specific activity, mutant H46A Salmonella enterica subsp. enterica serovar Typhimurium
2.7.1.156 0.00000008
-
 GTP:kinase specific activity, mutant H46N Salmonella enterica subsp. enterica serovar Typhimurium
2.7.1.156 0.000251
-
 GTP:kinase specific activity, mutant H45A Salmonella enterica subsp. enterica serovar Typhimurium
2.7.1.156 0.001166
-
 GTP:kinase specific activity, wild-type Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.62 0.00000002
-
 GTP:guanylyltransferase specific activity, mutant H46A Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.62 0.00000003
-
 GTP:guanylyltransferase specific activity, mutant H46N Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.62 0.000205
-
 GTP:guanylyltransferase specific activity, mutant H45A Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.62 0.00067
-
 GTP:guanylyltransferase specific activity, wild-type Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.156 ATP + 5'-deoxyadenosylcobinamide highest kinase activity, product generation relative to adenosylcobinamide 102% Salmonella enterica subsp. enterica serovar Typhimurium 5'-deoxyadenosylcobinamide phosphate + ADP
-
 ?
2.7.1.156 ATP + 5'-deoxycytosylcobinamide about 65% of the activity with AdoCbi Salmonella enterica subsp. enterica serovar Typhimurium 5'-deoxycytosylcobinamide phosphate + ADP
-
 ?
2.7.1.156 ATP + 5'-deoxyinosylcobinamide about 55% of the activity with AdoCbi Salmonella enterica subsp. enterica serovar Typhimurium 5'-deoxyinosylcobinamide phosphate + ADP
-
 ?
2.7.1.156 ATP + adenosylcobinamide
-
 Salmonella enterica subsp. enterica serovar Typhimurium adenosylcobinamide phosphate + ADP
-
 ?
2.7.1.156 ATP + adenosylcobinamide late steps of cobalamin biosynthesis, involved in de novo synthesis of vitamin B12, nucleotide loop assembly, enzyme functions only if Salmonella typhimurium is assimilating cobinamide from its environment, CobU becomes less active during the kinase reaction due to the formation of a covalent CobU-AMP complex that holds CobU in an altered conformation Salmonella enterica subsp. enterica serovar Typhimurium adenosylcobinamide phosphate + ADP
-
 ?
2.7.1.156 dATP + adenosylcobinamide
-
 Salmonella enterica subsp. enterica serovar Typhimurium adenosylcobinamide phosphate + dADP
-
 ?
2.7.1.156 dGTP + adenosylcobinamide
-
 Salmonella enterica subsp. enterica serovar Typhimurium adenosylcobinamide phosphate + dGDP
-
 ?
2.7.1.156 GTP + adenosylcobinamide
-
 Salmonella enterica subsp. enterica serovar Typhimurium adenosylcobinamide phosphate + GDP
-
 ?
2.7.1.156 additional information (CN)2Cbi is no substrate, no product formation with CTP, UTP or dCTP Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
2.7.7.62 GTP + adenosylcobinamide phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium diphosphate + adenosylcobinamide-GDP
-
 ?
2.7.7.62 GTP + adenosylcobinamide phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium diphosphate + adenosylcobinamide-GDP late steps of cobalamin biosynthesis, involved in de novo synthesis of vitamin B12, nucleotide loop assembly, required for assimilation of exogenous cobinamide and de novo synthesis of adenosylcobalamin, reaction proceeds via a covalently modified CobU-GMP intermediate ?
2.7.7.62 additional information CobU is a bifunctional enzyme that has both kinase EC 27.1.1.156 and guanyltransferase EC 2.7.7.62 activities Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.156 adenosylcobinamide kinase
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.1.156 CobU
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.1.156 CobU AdoCbi kinase
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.62 adenosylcobinamide phosphate guanylyltransferase
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.62 AdoCbi-P guanylyltransferase
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.62 CobU
-
 Salmonella enterica subsp. enterica serovar Typhimurium