EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.156 | GTP | stimulates kinase activity | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.1.156 | overexpressed in strain JE3207 | Salmonella enterica subsp. enterica serovar Typhimurium |
2.7.7.62 | overexpressed in strain JE3207 | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | General Stability | Organism |
---|---|---|
2.7.1.156 | substrates provides little protection from inhibition by DTNB | Salmonella enterica subsp. enterica serovar Typhimurium |
2.7.7.62 | substrates provides little protection from inhibition by DTNB, GTP provides slight protection from inhibition of the guanylyltransferase activity | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.156 | DTNB | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
2.7.7.62 | DTNB | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.156 | 0.066 | - |
5'-deoxyadenosylcobinamide | pH 8.8, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.7.1.156 | 0.206 | - |
ATP | pH 8.8, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.7.7.62 | 0.0213 | - |
GTP | pH 8.8, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.7.7.62 | 0.102 | - |
5'-deoxyadenosylcobinamide phosphate | pH 8.8, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.1.156 | 19700 | - |
predicted mass, based on DNA sequence | Salmonella enterica subsp. enterica serovar Typhimurium |
2.7.1.156 | 22000 | - |
2 * 22000, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
2.7.1.156 | 49000 | - |
gel filtration | Salmonella enterica subsp. enterica serovar Typhimurium |
2.7.7.62 | 19700 | - |
predicted mass, based on DNA sequence | Salmonella enterica subsp. enterica serovar Typhimurium |
2.7.7.62 | 22000 | - |
2 * 22000, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
2.7.7.62 | 49000 | - |
gel filtration | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.156 | ATP + adenosylcobinamide | Salmonella enterica subsp. enterica serovar Typhimurium | - |
adenosylcobinamide phosphate + ADP | - |
? | |
2.7.1.156 | ATP + adenosylcobinamide | Salmonella enterica subsp. enterica serovar Typhimurium | assembly of the nucleotide loop of cobalamin | adenosylcobinamide phosphate + ADP | - |
? | |
2.7.1.156 | GTP + adenosylcobinamide | Salmonella enterica subsp. enterica serovar Typhimurium | - |
adenosylcobinamide phosphate + GDP | - |
? | |
2.7.1.156 | GTP + adenosylcobinamide | Salmonella enterica subsp. enterica serovar Typhimurium | assembly of the nucleotide loop of cobalamin | adenosylcobinamide phosphate + GDP | - |
? | |
2.7.7.62 | GTP + adenosylcobinamide phosphate | Salmonella enterica subsp. enterica serovar Typhimurium | - |
diphosphate + adenosylcobinamide-GDP | - |
? | |
2.7.7.62 | GTP + adenosylcobinamide phosphate | Salmonella enterica subsp. enterica serovar Typhimurium | assembly of the nucleotide loop of cobalamin | diphosphate + adenosylcobinamide-GDP | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.156 | Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
2.7.7.62 | Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
EC Number | Oxidation Stability | Organism |
---|---|---|
2.7.1.156 | in presence of oxygen kinase activity is lost | Salmonella enterica subsp. enterica serovar Typhimurium |
2.7.7.62 | in presence of oxygen guanylyltransferase activity is lost | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.156 | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
2.7.7.62 | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.1.156 | 0.00013 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
2.7.7.62 | 0.000202 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Storage Stability | Organism |
---|---|---|
2.7.1.156 | 4°C, stored under a N2 headspace, retains 98% of its activity for at least 3 weeks | Salmonella enterica subsp. enterica serovar Typhimurium |
2.7.7.62 | 4°C, stored under a N2 headspace, retains 98% of its activity for at least 3 weeks | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.156 | ATP + 5'-deoxyadenosylcobinamide | - |
Salmonella enterica subsp. enterica serovar Typhimurium | 5'-deoxyadenosylcobinamide phosphate + ADP | - |
? | |
2.7.1.156 | ATP + adenosylcobinamide | - |
Salmonella enterica subsp. enterica serovar Typhimurium | adenosylcobinamide phosphate + ADP | - |
? | |
2.7.1.156 | ATP + adenosylcobinamide | assembly of the nucleotide loop of cobalamin | Salmonella enterica subsp. enterica serovar Typhimurium | adenosylcobinamide phosphate + ADP | - |
? | |
2.7.1.156 | GTP + adenosylcobinamide | - |
Salmonella enterica subsp. enterica serovar Typhimurium | adenosylcobinamide phosphate + GDP | - |
? | |
2.7.1.156 | GTP + adenosylcobinamide | assembly of the nucleotide loop of cobalamin | Salmonella enterica subsp. enterica serovar Typhimurium | adenosylcobinamide phosphate + GDP | - |
? | |
2.7.1.156 | additional information | CobU does not have any detectable ATP:adenosyltransferase activity | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
2.7.7.62 | GTP + adenosylcobinamide phosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | diphosphate + adenosylcobinamide-GDP | - |
? | |
2.7.7.62 | GTP + adenosylcobinamide phosphate | assembly of the nucleotide loop of cobalamin | Salmonella enterica subsp. enterica serovar Typhimurium | diphosphate + adenosylcobinamide-GDP | - |
? | |
2.7.7.62 | additional information | CobU is a bifunctional enzyme that has both kinase EC 27.1.1.156 and guanyltransferase EC 2.7.7.62 activities, ATP does not serve as an efficient substrate for the CobU transferase activity, CobU does not have any detectable ATP:adenosyltransferase activity | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.1.156 | dimer | 2 * 22000, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
2.7.7.62 | dimer | 2 * 22000, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.1.156 | bifunctional CobU enzyme | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
2.7.1.156 | CobU | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
2.7.1.156 | CobU protein | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
2.7.7.62 | bifunctional CobU enzyme | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
2.7.7.62 | CobU | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
2.7.7.62 | CobU protein | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.156 | 37 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
2.7.7.62 | 37 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.156 | 25 | 60 | retains 50% activity in this range, temperatures above 60°C results in drastic decrease of activity | Salmonella enterica subsp. enterica serovar Typhimurium |
2.7.7.62 | 25 | 60 | retains 50% activity in this range, temperatures above 60°C results in drastic decrease of activity | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.156 | 0.191 | - |
ATP | pH 8.8, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.7.1.156 | 0.251 | - |
5'-deoxyadenosylcobinamide | pH 8.8, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.7.7.62 | 0.058 | - |
GTP | pH 8.8, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.7.7.62 | 0.093 | - |
5'-deoxyadenosylcobinamide phosphate | pH 8.8, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.156 | 8.8 | 9 | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
2.7.7.62 | 8.8 | 9 | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.156 | 8.5 | 9.5 | 50% activity retains in this range | Salmonella enterica subsp. enterica serovar Typhimurium |
2.7.7.62 | 8.5 | 9.5 | 50% activity retains in this range | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
2.7.1.156 | Salmonella enterica subsp. enterica serovar Typhimurium | predicted pI for the denatured protein | - |
5.3 |
2.7.1.156 | Salmonella enterica subsp. enterica serovar Typhimurium | experimetally determined isoelectric point | - |
7.2 |
2.7.1.156 | Salmonella enterica subsp. enterica serovar Typhimurium | experimetally determined isoelectric point | - |
7.5 |
2.7.7.62 | Salmonella enterica subsp. enterica serovar Typhimurium | predicted pI for the denatured protein | - |
5.3 |
2.7.7.62 | Salmonella enterica subsp. enterica serovar Typhimurium | experimetally determined isoelectric point | - |
7.2 |
2.7.7.62 | Salmonella enterica subsp. enterica serovar Typhimurium | experimetally determined isoelectric point | - |
7.5 |