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Literature summary extracted from

  • Ueno, S.I.; Morino, H.; Sano, A.; Kakimoto, Y.
    Purification and characterization of D-3-aminoisobutyrate-pyruvate aminotransferase from rat liver (1990), Biochim. Biophys. Acta, 1033, 169-175.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.6.1.40 2-mercaptoethanol activation only in the presence of pyridoxal phosphate Rattus norvegicus
2.6.1.40 DTT activation Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.40 GSH weak Rattus norvegicus
2.6.1.40 L-cysteine
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.1.40 cytoplasm
-
 Rattus norvegicus 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.40 50000
-
 4 * 50000, reductive SDS-PAGE Rattus norvegicus
2.6.1.40 180000
-
 gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.40 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.40
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.40 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.40 1.14
-
 pH 8.8, 37°C Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.6.1.40 -20°C, concentrated enzyme solution, in 5% polyethylene glycol plus 1 mg/ml pyridoxal 5'-phosphate, several months Rattus norvegicus
2.6.1.40 4°C, concentrated enzyme solution, several days Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.40 (R)-3-amino-2-methylpropanoate + 2-oxo-n-butanoate
-
 Rattus norvegicus 2-methyl-3-oxopropanoate + 2-amino-n-butanoate
-
 ?
2.6.1.40 (R)-3-amino-2-methylpropanoate + 2-oxo-n-pentanoate
-
 Rattus norvegicus 2-methyl-3-oxopropanoate + norvaline
-
 ?
2.6.1.40 (R)-3-amino-2-methylpropanoate + glyoxylate
-
 Rattus norvegicus 2-methyl-3-oxopropanoate + glycine
-
 ?
2.6.1.40 (R)-3-amino-2-methylpropanoate + oxaloacetate
-
 Rattus norvegicus 2-methyl-3-oxopropanoate + L-aspartate
-
 ?
2.6.1.40 (R)-3-amino-2-methylpropanoate + pyruvate
-
 Rattus norvegicus 2-methyl-3-oxopropanoate + L-alanine
-
 ?
2.6.1.40 5-aminolevulinic acid + pyruvate
-
 Rattus norvegicus 5-oxolevulinic acid + L-alanine
-
 ?
2.6.1.40 arginine + pyruvate transamination at 40% the rate of (R)-3-amino-2-methylpropanoate Rattus norvegicus 2-oxo-5-guanidinovaleric acid + L-alanine
-
 ?
2.6.1.40 beta-alanine + pyruvate
-
 Rattus norvegicus malonic semialdehyde + L-alanine
-
 ?
2.6.1.40 DL-3-amino-n-butanoate + pyruvate
-
 Rattus norvegicus 3-oxobutanoate + L-alanine
-
 ?
2.6.1.40 glycine + pyruvate
-
 Rattus norvegicus glyoxylate + L-alanine
-
 ?
2.6.1.40 additional information overview Rattus norvegicus ?
-
 ?
2.6.1.40 NG,NG'-dimethyl-(S)-arginine + pyruvate
-
 Rattus norvegicus ? + L-alanine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.40 tetramer 4 * 50000, reductive SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.40 37
-
 assay at Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.40 pyridoxal 5'-phosphate requirement, tightly bound Rattus norvegicus