BRENDA - Enzyme Database show

Purification and characterization of D-3-aminoisobutyrate-pyruvate aminotransferase from rat liver

Ueno, S.I.; Morino, H.; Sano, A.; Kakimoto, Y.; Biochim. Biophys. Acta 1033, 169-175 (1990)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.6.1.40
2-mercaptoethanol
activation only in the presence of pyridoxal phosphate
Rattus norvegicus
2.6.1.40
DTT
activation
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.6.1.40
GSH
weak
Rattus norvegicus
2.6.1.40
L-cysteine
-
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.6.1.40
cytoplasm
-
Rattus norvegicus
5737
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.6.1.40
50000
-
4 * 50000, reductive SDS-PAGE
Rattus norvegicus
2.6.1.40
180000
-
gel filtration
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.6.1.40
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.6.1.40
-
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.6.1.40
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.6.1.40
1.14
-
pH 8.8, 37C
Rattus norvegicus
Storage Stability
EC Number
Storage Stability
Organism
2.6.1.40
-20C, concentrated enzyme solution, in 5% polyethylene glycol plus 1 mg/ml pyridoxal 5'-phosphate, several months
Rattus norvegicus
2.6.1.40
4C, concentrated enzyme solution, several days
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.40
(R)-3-amino-2-methylpropanoate + 2-oxo-n-butanoate
-
644430
Rattus norvegicus
2-methyl-3-oxopropanoate + 2-amino-n-butanoate
-
-
-
?
2.6.1.40
(R)-3-amino-2-methylpropanoate + 2-oxo-n-pentanoate
-
644430
Rattus norvegicus
2-methyl-3-oxopropanoate + norvaline
-
-
-
?
2.6.1.40
(R)-3-amino-2-methylpropanoate + glyoxylate
-
644430
Rattus norvegicus
2-methyl-3-oxopropanoate + glycine
-
-
-
?
2.6.1.40
(R)-3-amino-2-methylpropanoate + oxaloacetate
-
644430
Rattus norvegicus
2-methyl-3-oxopropanoate + L-aspartate
-
-
-
?
2.6.1.40
(R)-3-amino-2-methylpropanoate + pyruvate
-
644430
Rattus norvegicus
2-methyl-3-oxopropanoate + L-alanine
-
644430
Rattus norvegicus
?
2.6.1.40
5-aminolevulinic acid + pyruvate
-
644430
Rattus norvegicus
5-oxolevulinic acid + L-alanine
-
-
-
?
2.6.1.40
arginine + pyruvate
transamination at 40% the rate of (R)-3-amino-2-methylpropanoate
644430
Rattus norvegicus
2-oxo-5-guanidinovaleric acid + L-alanine
-
-
-
?
2.6.1.40
beta-alanine + pyruvate
-
644430
Rattus norvegicus
malonic semialdehyde + L-alanine
-
-
-
?
2.6.1.40
DL-3-amino-n-butanoate + pyruvate
-
644430
Rattus norvegicus
3-oxobutanoate + L-alanine
-
-
-
?
2.6.1.40
glycine + pyruvate
-
644430
Rattus norvegicus
glyoxylate + L-alanine
-
-
-
?
2.6.1.40
additional information
overview
644430
Rattus norvegicus
?
-
-
-
-
2.6.1.40
NG,NG'-dimethyl-(S)-arginine + pyruvate
-
644430
Rattus norvegicus
? + L-alanine
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.6.1.40
tetramer
4 * 50000, reductive SDS-PAGE
Rattus norvegicus
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.6.1.40
37
-
assay at
Rattus norvegicus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.6.1.40
pyridoxal 5'-phosphate
requirement, tightly bound
Rattus norvegicus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.6.1.40
2-mercaptoethanol
activation only in the presence of pyridoxal phosphate
Rattus norvegicus
2.6.1.40
DTT
activation
Rattus norvegicus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.6.1.40
pyridoxal 5'-phosphate
requirement, tightly bound
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.6.1.40
GSH
weak
Rattus norvegicus
2.6.1.40
L-cysteine
-
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.6.1.40
cytoplasm
-
Rattus norvegicus
5737
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.6.1.40
50000
-
4 * 50000, reductive SDS-PAGE
Rattus norvegicus
2.6.1.40
180000
-
gel filtration
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.6.1.40
-
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.6.1.40
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.6.1.40
1.14
-
pH 8.8, 37C
Rattus norvegicus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.6.1.40
-20C, concentrated enzyme solution, in 5% polyethylene glycol plus 1 mg/ml pyridoxal 5'-phosphate, several months
Rattus norvegicus
2.6.1.40
4C, concentrated enzyme solution, several days
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.40
(R)-3-amino-2-methylpropanoate + 2-oxo-n-butanoate
-
644430
Rattus norvegicus
2-methyl-3-oxopropanoate + 2-amino-n-butanoate
-
-
-
?
2.6.1.40
(R)-3-amino-2-methylpropanoate + 2-oxo-n-pentanoate
-
644430
Rattus norvegicus
2-methyl-3-oxopropanoate + norvaline
-
-
-
?
2.6.1.40
(R)-3-amino-2-methylpropanoate + glyoxylate
-
644430
Rattus norvegicus
2-methyl-3-oxopropanoate + glycine
-
-
-
?
2.6.1.40
(R)-3-amino-2-methylpropanoate + oxaloacetate
-
644430
Rattus norvegicus
2-methyl-3-oxopropanoate + L-aspartate
-
-
-
?
2.6.1.40
(R)-3-amino-2-methylpropanoate + pyruvate
-
644430
Rattus norvegicus
2-methyl-3-oxopropanoate + L-alanine
-
644430
Rattus norvegicus
?
2.6.1.40
5-aminolevulinic acid + pyruvate
-
644430
Rattus norvegicus
5-oxolevulinic acid + L-alanine
-
-
-
?
2.6.1.40
arginine + pyruvate
transamination at 40% the rate of (R)-3-amino-2-methylpropanoate
644430
Rattus norvegicus
2-oxo-5-guanidinovaleric acid + L-alanine
-
-
-
?
2.6.1.40
beta-alanine + pyruvate
-
644430
Rattus norvegicus
malonic semialdehyde + L-alanine
-
-
-
?
2.6.1.40
DL-3-amino-n-butanoate + pyruvate
-
644430
Rattus norvegicus
3-oxobutanoate + L-alanine
-
-
-
?
2.6.1.40
glycine + pyruvate
-
644430
Rattus norvegicus
glyoxylate + L-alanine
-
-
-
?
2.6.1.40
additional information
overview
644430
Rattus norvegicus
?
-
-
-
-
2.6.1.40
NG,NG'-dimethyl-(S)-arginine + pyruvate
-
644430
Rattus norvegicus
? + L-alanine
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.6.1.40
tetramer
4 * 50000, reductive SDS-PAGE
Rattus norvegicus
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.6.1.40
37
-
assay at
Rattus norvegicus