BRENDA - Enzyme Database show

Purification, characterization and inhibition of D-3-aminoisobutyrate aminotransferase from the rat liver

Tamaki, N.; Kaneko, M.; Mizota, C.; Kikugawa, M.; Fujimoto, S.; Eur. J. Biochem. 189, 39-45 (1990)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.6.1.40
6-Azauracil
weak, kinetics, not reversible by pyridoxal phosphate
Rattus norvegicus
2.6.1.40
6-azauridine
weak
Rattus norvegicus
2.6.1.40
Amino-oxyacetate
strong
Rattus norvegicus
2.6.1.40
D-cycloserine
-
Rattus norvegicus
2.6.1.40
gabaculine
-
Rattus norvegicus
2.6.1.40
additional information
no inhibitor: dihydrothymine, dihydrouracil, N-carbamoyl-beta-alanine, N-carbamoyl-DL-3-aminoisobutyrate; no inhibitor: precursors of beta-alanine or (R)-3-amino-2-methylpropanoate, i.e. orotic acid, uracil, thymine
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.6.1.40
0.45
-
pyruvate
pH 8.8, 37°C
Rattus norvegicus
2.6.1.40
0.81
-
beta-Alanine
-
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.6.1.40
cytoplasm
-
Rattus norvegicus
5737
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.6.1.40
52000
-
4 * 52000, SDS-PAGE
Rattus norvegicus
2.6.1.40
220000
-
gel filtration
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.6.1.40
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.6.1.40
-
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.6.1.40
liver
-
Rattus norvegicus
-
2.6.1.40
additional information
distribution in rat tissue
Rattus norvegicus
-
Storage Stability
EC Number
Storage Stability
Organism
2.6.1.40
4°C, 0.05 mg protein/ml, in 0.1 M potassium phosphate buffer, pH 7, 1 mM EDTA, 2 mM 2-mercaptoethanol, about 50% loss of activity within a few days, 80% loss within 1 week
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.40
(R)-3-amino-2-methylpropanoate + glyoxylate
-
644426
Rattus norvegicus
2-methyl-3-oxopropanoate + glycine
-
-
-
?
2.6.1.40
(R)-3-amino-2-methylpropanoate + oxaloacetate
-
644426
Rattus norvegicus
2-methyl-3-oxopropanoate + L-aspartate
-
-
-
?
2.6.1.40
(R)-3-amino-2-methylpropanoate + pyruvate
-
644426
Rattus norvegicus
2-methyl-3-oxopropanoate + L-alanine
-
644426
Rattus norvegicus
?
2.6.1.40
5-aminolevulinic acid + pyruvate
-
644426
Rattus norvegicus
5-oxolevulinic acid + L-alanine
-
-
-
?
2.6.1.40
beta-alanine + pyruvate
-
644426
Rattus norvegicus
malonic semialdehyde + L-alanine
-
-
-
?
2.6.1.40
additional information
overview
644426
Rattus norvegicus
?
-
-
-
-
2.6.1.40
additional information
no substrate: 2-oxoglutarate
644426
Rattus norvegicus
?
-
-
-
-
2.6.1.40
additional information
no substrate: phenylpyruvate
644426
Rattus norvegicus
?
-
-
-
-
2.6.1.40
NG,NG'-dimethyl-(S)-arginine + pyruvate
-
644426
Rattus norvegicus
? + L-alanine
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.6.1.40
tetramer
4 * 52000, SDS-PAGE; cross-linking experiments: tetramer composed of two dimers
Rattus norvegicus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.6.1.40
37
-
assay at
Rattus norvegicus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.6.1.40
additional information
-
pI: 6.7
Rattus norvegicus
2.6.1.40
9.5
-
-
Rattus norvegicus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.6.1.40
pyridoxal 5'-phosphate
-
Rattus norvegicus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.6.1.40
pyridoxal 5'-phosphate
-
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.6.1.40
6-Azauracil
weak, kinetics, not reversible by pyridoxal phosphate
Rattus norvegicus
2.6.1.40
6-azauridine
weak
Rattus norvegicus
2.6.1.40
Amino-oxyacetate
strong
Rattus norvegicus
2.6.1.40
D-cycloserine
-
Rattus norvegicus
2.6.1.40
gabaculine
-
Rattus norvegicus
2.6.1.40
additional information
no inhibitor: dihydrothymine, dihydrouracil, N-carbamoyl-beta-alanine, N-carbamoyl-DL-3-aminoisobutyrate; no inhibitor: precursors of beta-alanine or (R)-3-amino-2-methylpropanoate, i.e. orotic acid, uracil, thymine
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.6.1.40
0.45
-
pyruvate
pH 8.8, 37°C
Rattus norvegicus
2.6.1.40
0.81
-
beta-Alanine
-
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.6.1.40
cytoplasm
-
Rattus norvegicus
5737
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.6.1.40
52000
-
4 * 52000, SDS-PAGE
Rattus norvegicus
2.6.1.40
220000
-
gel filtration
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.6.1.40
-
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.6.1.40
liver
-
Rattus norvegicus
-
2.6.1.40
additional information
distribution in rat tissue
Rattus norvegicus
-
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.6.1.40
4°C, 0.05 mg protein/ml, in 0.1 M potassium phosphate buffer, pH 7, 1 mM EDTA, 2 mM 2-mercaptoethanol, about 50% loss of activity within a few days, 80% loss within 1 week
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.40
(R)-3-amino-2-methylpropanoate + glyoxylate
-
644426
Rattus norvegicus
2-methyl-3-oxopropanoate + glycine
-
-
-
?
2.6.1.40
(R)-3-amino-2-methylpropanoate + oxaloacetate
-
644426
Rattus norvegicus
2-methyl-3-oxopropanoate + L-aspartate
-
-
-
?
2.6.1.40
(R)-3-amino-2-methylpropanoate + pyruvate
-
644426
Rattus norvegicus
2-methyl-3-oxopropanoate + L-alanine
-
644426
Rattus norvegicus
?
2.6.1.40
5-aminolevulinic acid + pyruvate
-
644426
Rattus norvegicus
5-oxolevulinic acid + L-alanine
-
-
-
?
2.6.1.40
beta-alanine + pyruvate
-
644426
Rattus norvegicus
malonic semialdehyde + L-alanine
-
-
-
?
2.6.1.40
additional information
overview
644426
Rattus norvegicus
?
-
-
-
-
2.6.1.40
additional information
no substrate: 2-oxoglutarate
644426
Rattus norvegicus
?
-
-
-
-
2.6.1.40
additional information
no substrate: phenylpyruvate
644426
Rattus norvegicus
?
-
-
-
-
2.6.1.40
NG,NG'-dimethyl-(S)-arginine + pyruvate
-
644426
Rattus norvegicus
? + L-alanine
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.6.1.40
tetramer
4 * 52000, SDS-PAGE; cross-linking experiments: tetramer composed of two dimers
Rattus norvegicus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.6.1.40
37
-
assay at
Rattus norvegicus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.6.1.40
additional information
-
pI: 6.7
Rattus norvegicus
2.6.1.40
9.5
-
-
Rattus norvegicus