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Literature summary extracted from

  • Kanoh, H.; Ohno, K.
    Solubilization and purification of rat liver microsomal 1,2-diacylglycerol: CDP-choline cholinephosphotransferase and 1,2-diacylglycerol: CDP-ethanolamine ethanolaminephosphotransferase (1976), Eur. J. Biochem., 66, 201-210.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.8.1 deoxycholate activates Rattus norvegicus
2.7.8.1 Triton X-100 slightly stimulates with Mn2+, but not with Mg2+ as cofactor Rattus norvegicus
2.7.8.2 deoxycholate stimulates Rattus norvegicus
2.7.8.2 Phospholipids microsomal phospholipids required for maximal activity Rattus norvegicus

Application

EC Number Application Comment Organism
2.7.8.2 medicine exposure of cortical neurons to neurotoxic concentrations of N-methyl-D-aspartate strongly reduces enzymic activity Rattus norvegicus

General Stability

EC Number General Stability Organism
2.7.8.1 Triton X-100, stable to Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.8.1 CDP-choline
-
 Rattus norvegicus
2.7.8.1 ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetracetic acid
-
 Rattus norvegicus
2.7.8.1 Microsomal phospholipids
-
 Rattus norvegicus
2.7.8.2 CDPethanolamine
-
 Rattus norvegicus
2.7.8.2 Triton X-100 X-100 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.8.1 additional information
-
 additional information
-
 Rattus norvegicus
2.7.8.2 additional information
-
 additional information
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.8.1 microsome
-
 Rattus norvegicus
-
-
2.7.8.2 microsome
-
 Rattus norvegicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.8.1 Co2+ negligible effect Rattus norvegicus
2.7.8.1 Mg2+ can partially replace Mn2+ in activation Rattus norvegicus
2.7.8.1 Mn2+ much more effective than Mg2+ Rattus norvegicus
2.7.8.2 Mg2+ required Rattus norvegicus
2.7.8.2 Mg2+ enzyme separated into Mg2+-requiring and Mn2+-requiring components Rattus norvegicus
2.7.8.2 Mn2+ enzyme separated into Mg2+-requiring and Mn2+-requiring components Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.1 Rattus norvegicus
-
-
-
2.7.8.2 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.8.1 partial Rattus norvegicus
2.7.8.2
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.8.1 liver
-
 Rattus norvegicus
-
2.7.8.2 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.8.1 12.1
-
 30°C, pH 8.5 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.1 CDP-ethanolamine + 1,2-diacylglycerol
-
 Rattus norvegicus CMP + a phosphatidylethanolamine
-
 ?
2.7.8.2 CDP-choline + 1,2-diacylglycerol
-
 Rattus norvegicus CMP + a phosphatidylcholine
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.8.1 8 8.5
-
 Rattus norvegicus
2.7.8.2 8 8.5
-
 Rattus norvegicus