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Literature summary extracted from

  • Cornell, R.; MaxLennan, D.H.
    Solubilization and reconstitution of cholinephosphotransferase from sarcoplasmic reticulum: stabilization of solubilized enzyme by diacylglycerol and glycerol (1985), Biochim. Biophys. Acta, 821, 97-105.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.8.2 cholate inactivation at solubilization concentration, full recovery of activity after reconstituting the membrane by adding excess lipid and removing detergent by gel filtration, dialysis or absorption to Bio-Beads, if membrane is solubilized with octylglucoside or cholate at weight ratios of detergent:membrane protein of at least 10, the activity is irreversibly lost unless stabilizers are added with detergent, diacylglycerol and glycerol are effective stabilizers Oryctolagus cuniculus
2.7.8.2 n-octylglucoside inactivation at solubilization concentration, full recovery of activity after reconstituting the membrane by adding excess lipid and removing detergent by gel filtration, dialysis or absorption to Bio-Beads, if membrane is solubilized with octylglucoside or cholate at weight ratios of detergent:membrane protein of at least 10, the activity is irreversibly lost unless stabilizers are added with detergent, diacylglycerol and glycerol are effective stabilizers Oryctolagus cuniculus
2.7.8.2 SDS inactivation at solubilization concentration, full recovery of activity after reconstituting the membrane by adding excess lipid and removing detergent by gel filtration, dialysis or absorption to Bio-Beads Oryctolagus cuniculus
2.7.8.2 Triton X-100 inactivation at solubilization concentration, full recovery of activity after reconstituting the membrane by adding excess lipid (soybean) and removing detergent by gel filtration, dialysis or absorption to Bio-Beads; X-100 Oryctolagus cuniculus
2.7.8.2 Tween-20 inactivation at solubilization concentration, full recovery of activity after reconstituting the membrane by adding excess lipid (soybean) and removing detergent by gel filtration, dialysis or absorption to Bio-Beads Oryctolagus cuniculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.8.2 sarcoplasmic reticulum
-
 Oryctolagus cuniculus 16529
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.8.2 CDP-choline + sn-1,2-diacylglycerol Oryctolagus cuniculus final reaction in synthesis of phosphatidylcholine ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.2 Oryctolagus cuniculus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.8.2 skeletal muscle
-
 Oryctolagus cuniculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.2 CDP-choline + 1,2-diacylglycerol
-
 Oryctolagus cuniculus CMP + a phosphatidylcholine
-
 ?
2.7.8.2 CDP-choline + sn-1,2-diacylglycerol final reaction in synthesis of phosphatidylcholine Oryctolagus cuniculus ?
-
 ?