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Literature summary extracted from

  • Ishidate, K.; Matsuo, R.; Nakazawa, Y.
    CDPcholine:1,2-diacylglycerol cholinephosphotransferase from rat liver microsomes. I. Solubilization and characterization of the partially purified enzyme and the possible existence of an endogenous inhibitor (1993), Lipids, 28, 89-96.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.8.2 CHAPS irreversible inactivation at the solubilization step Rattus norvegicus
2.7.8.2 additional information heat-labile, nondialyzable endogenous inhibitor may act at the binding step of the enzyme to its lipid substrate Rattus norvegicus
2.7.8.2 n-octylglucoside irreversible inactivation at the solubilization step Rattus norvegicus
2.7.8.2 Triton X-100 irreversible inactivation at the solubilization step; X-100 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.8.2 microsome
-
 Rattus norvegicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.8.2 Mg2+ requires Mg2+ rather than Mn2+, particularly at physiological concentrations less than 5 mM Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.2 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.8.2
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.8.2 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.8.2 0.13
-
 37°C, pH 8.5 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.2 CDP-choline + 1,2-diacylglycerol
-
 Rattus norvegicus CMP + a phosphatidylcholine
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.8.2 37
-
 assay at Rattus norvegicus