Literature summary extracted from

  • Coleman, R.; Bell, R.M.
    Phospholipid synthesis in isolated fat cells. Studies of microsomal diacylglycerol cholinephosphotransferase and diacylglycerol ethanolaminephosphotransferase activities (1977), J. Biol. Chem., 252, 3050-3056.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.8.2 ethylene glycol bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid stimulates Rattus norvegicus
2.7.8.2 EGTA 0.5 mM, stimulates Rattus norvegicus
2.7.8.2 Phospholipase C 23°C, 10 min, slight stimulation Rattus norvegicus
2.7.8.1 EGTA stimulates Rattus norvegicus
2.7.8.1 bovine serum albumin 1 mg/ml, 2fold increase of activity Rattus norvegicus
2.7.8.1 pospholipase C 10 min, 23°C, 26% stimulation Rattus norvegicus

General Stability

EC Number General Stability Organism
2.7.8.2 freezing and thawing the microsomal preparation 5times destroys activity by 50% Rattus norvegicus
2.7.8.1 trypsin, 70%, 0.9 min, activity remains stable Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.8.1 acetone
-
Rattus norvegicus
2.7.8.1 Ca2+ CaCl2 Rattus norvegicus
2.7.8.1 CDP
-
Rattus norvegicus
2.7.8.1 CDP-choline
-
Rattus norvegicus
2.7.8.1 CMP
-
Rattus norvegicus
2.7.8.1 CTP
-
Rattus norvegicus
2.7.8.1 dioxane
-
Rattus norvegicus
2.7.8.1 dithiothreitol
-
Rattus norvegicus
2.7.8.1 methanol
-
Rattus norvegicus
2.7.8.1 palmitoyl-CoA
-
Rattus norvegicus
2.7.8.1 Triton WR 1339
-
Rattus norvegicus
2.7.8.1 Tween 20
-
Rattus norvegicus
2.7.8.2 1-butanol
-
Rattus norvegicus
2.7.8.2 acetone
-
Rattus norvegicus
2.7.8.2 Ca2+
-
Rattus norvegicus
2.7.8.2 CDP
-
Rattus norvegicus
2.7.8.2 CDPethanolamine
-
Rattus norvegicus
2.7.8.2 dithiothreitol
-
Rattus norvegicus
2.7.8.2 methanol
-
Rattus norvegicus
2.7.8.2 palmitoyl-CoA
-
Rattus norvegicus
2.7.8.2 Triton X-100 WR 1339; X-100 Rattus norvegicus
2.7.8.2 Trypsin
-
Rattus norvegicus
2.7.8.2 Tween-20
-
Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.8.2 additional information
-
additional information
-
Rattus norvegicus
2.7.8.2 0.0057
-
didodecanoylglycerol 23°C, pH 8.5 Rattus norvegicus
2.7.8.2 0.118
-
dimyristoyl-sn-glycerol 23°C, pH 8.5 Rattus norvegicus
2.7.8.2 0.0141
-
didecanoylglycerol 23°C, pH 8.5 Rattus norvegicus
2.7.8.1 0.0118
-
1,2-dioleoylglycerol 23°C, pH 8.5 Rattus norvegicus
2.7.8.2 0.0239
-
CDP-choline cosubstrate 1,2-dioleoyl-sn-glycerol, 23°C, pH 8.5 Rattus norvegicus
2.7.8.2 0.0681
-
dihexanoylglycerol 23°C, pH 8.5 Rattus norvegicus
2.7.8.1 0.0183
-
CDP-ethanolamine cosubstrate 1,2-dioleoyl-sn-glycerol Rattus norvegicus
2.7.8.2 0.0405
-
bacterial 1,2-diacyl-sn-glycerol 23°C, pH 8.5 Rattus norvegicus
2.7.8.2 0.0509
-
1,2-dioleoyl-sn-glycerol 23°C, pH 8.5 Rattus norvegicus
2.7.8.2 0.0314
-
dihexadecanoylglycerol 23°C, pH 8.5 Rattus norvegicus
2.7.8.2 0.0501
-
dioctadecenoylglycerol 23°C, pH 8.5 Rattus norvegicus
2.7.8.2 0.125 0.126 1-stearoyl-2-oleoyl-sn-glycerol 23°C, pH 8.5 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.8.2 microsome
-
Rattus norvegicus
-
-
2.7.8.1 microsome
-
Rattus norvegicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.8.2 Mg2+ required Rattus norvegicus
2.7.8.2 Mn2+ can partially replace Mg2+ in activation Rattus norvegicus
2.7.8.1 Mg2+ Mn2+ or Mg2+ required Rattus norvegicus
2.7.8.1 Mn2+ Mn2+ or Mg2+ required Rattus norvegicus
2.7.8.1 Mn2+ much more effective than Mg2+ Rattus norvegicus
2.7.8.1 Mg2+ completely dependent on MgCl2 Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.2 Rattus norvegicus
-
-
-
2.7.8.1 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.8.2 liver
-
Rattus norvegicus
-
2.7.8.2 brain
-
Rattus norvegicus
-
2.7.8.2 lung fetal Rattus norvegicus
-
2.7.8.2 adipocyte
-
Rattus norvegicus
-
2.7.8.2 mucosa of intestine Rattus norvegicus
-
2.7.8.1 liver
-
Rattus norvegicus
-
2.7.8.1 brain
-
Rattus norvegicus
-
2.7.8.1 adipocyte
-
Rattus norvegicus
-
2.7.8.1 mucosa of intestine Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.2 additional information specificity overview Rattus norvegicus ?
-
?
2.7.8.2 additional information no substrate: 1,3-diC18:1-glycerol ether Rattus norvegicus ?
-
?
2.7.8.2 additional information no substrate: 1,2-diC18:1-glycerol ether Rattus norvegicus ?
-
?
2.7.8.2 additional information substrates are 1,2-diacylglycerols containing fatty acids 6, 12 and 14 carbons in length Rattus norvegicus ?
-
?
2.7.8.1 additional information 1,2-diacylglycerols containing fatty acids 6, 12 and 14 carbons in length are no substrates Rattus norvegicus ?
-
?
2.7.8.2 CDP-choline + 1,2-diacylglycerol
-
Rattus norvegicus CMP + a phosphatidylcholine
-
?
2.7.8.2 CDP-choline + 1,2-dioleoyl-sn-glycerol
-
Rattus norvegicus CMP + 1,2-dioleoyl-sn-glycero-3-phosphocholine
-
?
2.7.8.2 CDP-choline + dihexanoylglycerol
-
Rattus norvegicus CMP + dihexanoylglycerophosphocholine
-
?
2.7.8.2 CDP-choline + didecanoylglycerol
-
Rattus norvegicus CMP + didecanoylglycerophosphocholine
-
?
2.7.8.1 CDP-ethanolamine + 1,2-diacylglycerol
-
Rattus norvegicus CMP + a phosphatidylethanolamine
-
?
2.7.8.1 CDP-ethanolamine + 1,2-dioleoylglycerol highest activity Rattus norvegicus CMP + dioleoylphosphatidylethanolamine
-
?
2.7.8.1 CDP-ethanolamine + 1-stearoyl-2-oleoylglycerol
-
Rattus norvegicus CMP + 1-stearoyl-2-oleoylphosphatidylethanolamine
-
?
2.7.8.2 CDP-choline + 1-stearoyl-2-oleoyl-sn-glycerol
-
Rattus norvegicus CMP + 1-stearoyl-2-oleoyl-sn-glycero-3-phosphocholine
-
?
2.7.8.2 CDP-choline + bacterial 1,2-diacyl-sn-glycerol
-
Rattus norvegicus CMP + phosphatidylethanolamine
-
?
2.7.8.2 CDP-choline + didodecanoylglycerol
-
Rattus norvegicus CMP + didodecanoylglycerophosphocholine
-
?
2.7.8.2 CDP-choline + dimyristoyl-sn-glycerol
-
Rattus norvegicus CMP + dimyristoyl-sn-glycerophosphocholine
-
?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.8.2 37
-
19 min, stable, microsomal preparation Rattus norvegicus
2.7.8.1 37
-
stable for 19 min, heating of microsomes Rattus norvegicus
2.7.8.2 49
-
15 min, 50% loss of activity, microsomal preparation Rattus norvegicus
2.7.8.1 49
-
4 min, more than 50% loss of activity Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.8.2 8.5 9.3
-
Rattus norvegicus
2.7.8.1 8.5 9.3
-
Rattus norvegicus