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Literature summary extracted from

  • Anavoranich, S.; Varin, L.; Gulick, P.; Ibrahim, R.
    Cloning and regulation of flavonol 3-sulfotransferase in cell-suspension cultures of Flaveria bidentis (1994), Plant Physiol., 106, 485-491.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.2.25
-
 Flaveria bidentis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.2.25 quercetin 3-sulfate in cultures, up to 50% decrease in enzymic activity Flaveria bidentis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.25 0.0003
-
 quercetin recombinant enzyme Flaveria bidentis
2.8.2.25 0.0004
-
 3'-phosphoadenylylsulfate cosubstrate quercetin, recombinant enzyme Flaveria bidentis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.2.25 3'-phosphoadenylylsulfate + quercetin Flaveria bidentis
-
 adenosine 3',5'-bisphosphate + quercetin 3-sulfate
-
 ?
2.8.2.25 additional information Flaveria bidentis involved in biosynthesis of polysulfated flavonols ?
-
 ?
2.8.2.25 additional information Flaveria bidentis first step in biosynthesis of flavonol polysulfates ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.25 Flaveria bidentis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.25 partial Flaveria bidentis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.2.25 callus
-
 Flaveria bidentis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.25 3'-phosphoadenylylsulfate + isorhamnetin 10% of activity compared to quercetin Flaveria bidentis adenosine 3',5'-bisphosphate + isorhamnetin 3-sulfate
-
 ?
2.8.2.25 3'-phosphoadenylylsulfate + quercetin
-
 Flaveria bidentis adenosine 3',5'-bisphosphate + quercetin 3-sulfate
-
 ?
2.8.2.25 3'-phosphoadenylylsulfate + quercetin best substrate Flaveria bidentis adenosine 3',5'-bisphosphate + quercetin 3-sulfate
-
 ?
2.8.2.25 3'-phosphoadenylylsulfate + rhamnetin 75% of activity compared to quercetin Flaveria bidentis adenosine 3',5'-bisphosphate + rhamnetin 3-sulfate
-
 ?
2.8.2.25 additional information involved in biosynthesis of polysulfated flavonols Flaveria bidentis ?
-
 ?
2.8.2.25 additional information first step in biosynthesis of flavonol polysulfates Flaveria bidentis ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.25 6.5
-
 and pH 8.5, two maxima Flaveria bidentis
2.8.2.25 8.5
-
 and pH 6.5, two maxima Flaveria bidentis