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Literature summary extracted from

  • Varin, L.
    Enzymatic synthesis of sulphated flavonois in Flaveria spp. (1988), Bull. Liaison-Groupe Polyphenols, 14, 248-257.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.25 0.0002
-
 Rhamnetin
-
 Flaveria chlorifolia
2.8.2.25 0.0002
-
 3'-phosphoadenylylsulfate cosubstrate rhamnetin Flaveria chlorifolia
2.8.2.26 0.00029
-
 quercetin 3-sulfate
-
 Flaveria chlorifolia
2.8.2.26 0.00035
-
 3'-phosphoadenylylsulfate
-
 Flaveria chlorifolia
2.8.2.27 0.00036
-
 quercetin 3-sulfate
-
 Flaveria chlorifolia
2.8.2.27 0.00038
-
 3'-phosphoadenylylsulfate
-
 Flaveria chlorifolia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.2.25 35000
-
 gel filtration Flaveria chlorifolia
2.8.2.26 35000
-
 gel filtration Flaveria chlorifolia

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.2.25 3'-phosphoadenylylsulfate + quercetin Flaveria chlorifolia
-
 adenosine 3',5'-bisphosphate + quercetin 3-sulfate
-
 ?
2.8.2.25 additional information Flaveria chlorifolia involved in biosynthesis of polysulfated flavonols ?
-
 ?
2.8.2.26 3'-phosphoadenylylsulfate + quercetin 3-sulfate Flaveria chlorifolia
-
 adenosine 3',5'-bisphosphate + quercetin 3,3'-bissulfate
-
 ?
2.8.2.26 additional information Flaveria chlorifolia sulfation at 3'-positions is second step in formation of polysulfated flavonoids ?
-
 ?
2.8.2.27 3'-phosphoadenylylsulfate + quercetin 3-sulfate Flaveria chlorifolia
-
 quercetin 3,4'-disulfate + adenosine 3',5'-bisphosphate
-
 ?
2.8.2.27 additional information Flaveria chlorifolia involved in biosynthesis of polysulfated flavonoids ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.25 Flaveria chlorifolia
-
-
-
2.8.2.26 Flaveria chlorifolia
-
-
-
2.8.2.27 Flaveria chlorifolia
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.25 partial Flaveria chlorifolia
2.8.2.26 partial Flaveria chlorifolia
2.8.2.27 partial Flaveria chlorifolia

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.2.25 plant
-
 Flaveria chlorifolia
-
2.8.2.26 seed
-
 Flaveria chlorifolia
-
2.8.2.27 seed
-
 Flaveria chlorifolia
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.2.25 0.00027
-
-
 Flaveria chlorifolia
2.8.2.27 0.002
-
-
 Flaveria chlorifolia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.25 3'-phosphoadenylylsulfate + eupatin
-
 Flaveria chlorifolia adenosine 3',5'-bisphosphate + eupatin 3-sulfate
-
 ?
2.8.2.25 3'-phosphoadenylylsulfate + isorhamnetin
-
 Flaveria chlorifolia adenosine 3',5'-bisphosphate + isorhamnetin 3-sulfate
-
 ?
2.8.2.25 3'-phosphoadenylylsulfate + kaempferol
-
 Flaveria chlorifolia adenosine 3',5'-bisphosphate + kaempferol 3-sulfate
-
 ?
2.8.2.25 3'-phosphoadenylylsulfate + patuletin
-
 Flaveria chlorifolia adenosine 3',5'-bisphosphate + patuletin 3-sulfate
-
 ?
2.8.2.25 3'-phosphoadenylylsulfate + quercetin
-
 Flaveria chlorifolia adenosine 3',5'-bisphosphate + quercetin 3-sulfate
-
 ?
2.8.2.25 3'-phosphoadenylylsulfate + rhamnetin
-
 Flaveria chlorifolia adenosine 3',5'-bisphosphate + rhamnetin 3-sulfate
-
 ?
2.8.2.25 additional information quercetagetin, gossypetin, myricetin or galangin are no substrates Flaveria chlorifolia ?
-
 ?
2.8.2.25 additional information involved in biosynthesis of polysulfated flavonols Flaveria chlorifolia ?
-
 ?
2.8.2.26 3'-phosphoadenylylsulfate + quercetin 3-sulfate
-
 Flaveria chlorifolia adenosine 3',5'-bisphosphate + quercetin 3,3'-bissulfate
-
 ?
2.8.2.26 3'-phosphoadenylylsulfate + tamarixetin 3-sulfate
-
 Flaveria chlorifolia adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.26 additional information kaempferol 3-sulfate, isorhamnetin 3-sulfate or other flavonol aglycones are no substrates Flaveria chlorifolia ?
-
 ?
2.8.2.26 additional information sulfation at 3'-positions is second step in formation of polysulfated flavonoids Flaveria chlorifolia ?
-
 ?
2.8.2.27 3'-phosphoadenylylsulfate + isorhamnetin 3-sulfate
-
 Flaveria chlorifolia isorhamnetin 3,4'-disulfate + adenosine 3',5'-bisphosphate
-
 ?
2.8.2.27 3'-phosphoadenylylsulfate + kaempferol 3-sulfate
-
 Flaveria chlorifolia kaempferol 3,4'-disulfate + adenosine 3',5'-bisphosphate
-
 ?
2.8.2.27 3'-phosphoadenylylsulfate + quercetin 3-sulfate
-
 Flaveria chlorifolia quercetin 3,4'-disulfate + adenosine 3',5'-bisphosphate
-
 ?
2.8.2.27 additional information tamarixetin 3-sulfate, flavonol aglycones, or adenosine 5'-phosphosulfate are no substrates Flaveria chlorifolia ?
-
 ?
2.8.2.27 additional information involved in biosynthesis of polysulfated flavonoids Flaveria chlorifolia ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.25 6.5
-
 and pH 8.5, two maxima Flaveria chlorifolia
2.8.2.25 8.5
-
 and pH 6.5, two maxima Flaveria chlorifolia
2.8.2.26 7.5
-
-
 Flaveria chlorifolia
2.8.2.27 7.5
-
-
 Flaveria chlorifolia