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Literature summary extracted from

  • Dowhan, W.; Hirabayashi, T.
    Phosphatidylglycerophosphate synthase from Escherichia coli (1981), Methods Enzymol., 71, 555-561.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.8.5 Triton X-100 0.2% Escherichia coli
2.7.8.5 Triton X-100 maximal activity is dependent on Triton X-100 Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.8.5 CDP-diacylglycerol uncompetitive at high concentration Escherichia coli
2.7.8.5 Mg2+ required, Km: 50 mM, inhibition above 150 mM Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.8.5 0.034
-
 dCDP-diacylglycerol 37°C, pH 8.0 Escherichia coli
2.7.8.5 0.046
-
 CDP-diacylglycerol 37°C, pH 8.0 Escherichia coli
2.7.8.5 0.32
-
 sn-glycerol 3-phosphate pH 8.0, 37°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.8.5 Mg2+ absolute requirement for a divalent metal, Mg2+ required, Km: 50 mM, inhibition above 150 mM Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.8.5 24000
-
 x * 24000, SDS-PAGE   Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.5 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.8.5
-
 Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.8.5 CDP-diacylglycerol + sn-glycerol 3-phosphate = CMP + 1-(3-sn-phosphatidyl)-sn-glycerol 3-phosphate ordered sequential bi-bi reaction Escherichia coli
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.8.5 18.6
-
 37°C, pH 8.0 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.5 CDP-diacylglycerol + sn-glycero-3-phosphate
-
 Escherichia coli CMP + phosphatidylglycerophosphate
-
 ?
2.7.8.5 dCDP-diacylglycerol + sn-glycero-3-phosphate
-
 Escherichia coli dCMP + phosphatidylglycerophosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.8.5 ? x * 24000, SDS-PAGE   Escherichia coli

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.8.5 37
-
 assay at Escherichia coli