BRENDA - Enzyme Database

Purification and characterization of cob(II)yrinic acid a,c-diamide reductase from Pseudomonas denitrificans

Blanche F.; Maton, L.; Debussche, L.; Thibaut, D.; J. Bacteriol. 174, 7452-7454 (1992)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.16.8.1
0.0059
-
FMN
0.2 M Tris-HCl buffer, pH 8.0, 30°C
Pseudomonas denitrificans (nomen rejiciendum)
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.16.8.1
16000
-
2 * 16000, SDS-PAGE
Pseudomonas denitrificans (nomen rejiciendum)
1.16.8.1
30000
-
gel permeation HPLC
Pseudomonas denitrificans (nomen rejiciendum)
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.16.8.1
2 cob(II)yrinic acid a,c-diamide + FMN
Pseudomonas denitrificans (nomen rejiciendum)
-
cob(I)yrinic acid a,c-diamide + FMNH2
-
-
?
1.16.8.1
cob(II)yrinic acid a,c-diamide + FMN
Pseudomonas denitrificans (nomen rejiciendum)
-
?
-
Pseudomonas denitrificans (nomen rejiciendum)
?
6.3.5.10
ATP + adenosylcobyrinic acid a,c-diamide + L-glutamine + H2O
Pseudomonas denitrificans (nomen rejiciendum)
-
ADP + phosphate + adenosylcobyric acid + L-glutamate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.16.8.1
Pseudomonas denitrificans (nomen rejiciendum)
-
-
-
6.3.5.10
Pseudomonas denitrificans (nomen rejiciendum)
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.16.8.1
6,300-fold purification including Mono Q and FMN agarose chromatography
Pseudomonas denitrificans (nomen rejiciendum)
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.16.8.1
2 cob(II)yrinic acid a,c-diamide + FMN
-
644281
Pseudomonas denitrificans (nomen rejiciendum)
cob(I)yrinic acid a,c-diamide + FMNH2
-
-
-
?
1.16.8.1
cob(II)alamin + FMN
41% activity compared to cob(II)yric acid
644281
Pseudomonas denitrificans (nomen rejiciendum)
?
-
644281
Pseudomonas denitrificans (nomen rejiciendum)
?
1.16.8.1
cob(II)inamide + FMN
67-48% activity compared to cob(II)yric acid, percentage depends on method of activity detection
644281
Pseudomonas denitrificans (nomen rejiciendum)
?
-
644281
Pseudomonas denitrificans (nomen rejiciendum)
?
1.16.8.1
cob(II)inamide phosphate + FMN
66% activity compared to cob(II)yric acid
644281
Pseudomonas denitrificans (nomen rejiciendum)
?
-
644281
Pseudomonas denitrificans (nomen rejiciendum)
?
1.16.8.1
cob(II)yriC acid + FMN
-
644281
Pseudomonas denitrificans (nomen rejiciendum)
?
-
644281
Pseudomonas denitrificans (nomen rejiciendum)
?
1.16.8.1
cob(II)yrinic acid a,c-diamide + FMN
-
644281
Pseudomonas denitrificans (nomen rejiciendum)
cob(I)yrinic acid a,c-diamide + FMNH2
-
644281
Pseudomonas denitrificans (nomen rejiciendum)
?
1.16.8.1
cob(II)yrinic acid a,c-diamide + FMN
52-75% activity compared to cob(II)yric acid, percentage depends on method of activity detection
644281
Pseudomonas denitrificans (nomen rejiciendum)
cob(I)yrinic acid a,c-diamide + FMNH2
-
644281
Pseudomonas denitrificans (nomen rejiciendum)
?
1.16.8.1
cob(II)yrinic acid a,c-diamide + FMN
-
644281
Pseudomonas denitrificans (nomen rejiciendum)
?
-
644281
Pseudomonas denitrificans (nomen rejiciendum)
?
1.16.8.1
GDP-cob(II)inamide + FMN
71% activity compared to cob(II)yric acid
644281
Pseudomonas denitrificans (nomen rejiciendum)
?
-
644281
Pseudomonas denitrificans (nomen rejiciendum)
?
6.3.5.10
ATP + adenosylcobyrinic acid a,c-diamide + L-glutamine + H2O
-
644281
Pseudomonas denitrificans (nomen rejiciendum)
ADP + phosphate + adenosylcobyric acid + L-glutamate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.16.8.1
homodimer
2 * 16000, SDS-PAGE
Pseudomonas denitrificans (nomen rejiciendum)
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.16.8.1
0.0059
-
FMN
0.2 M Tris-HCl buffer, pH 8.0, 30°C
Pseudomonas denitrificans (nomen rejiciendum)
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.16.8.1
16000
-
2 * 16000, SDS-PAGE
Pseudomonas denitrificans (nomen rejiciendum)
1.16.8.1
30000
-
gel permeation HPLC
Pseudomonas denitrificans (nomen rejiciendum)
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.16.8.1
2 cob(II)yrinic acid a,c-diamide + FMN
Pseudomonas denitrificans (nomen rejiciendum)
-
cob(I)yrinic acid a,c-diamide + FMNH2
-
-
?
1.16.8.1
cob(II)yrinic acid a,c-diamide + FMN
Pseudomonas denitrificans (nomen rejiciendum)
-
?
-
Pseudomonas denitrificans (nomen rejiciendum)
?
6.3.5.10
ATP + adenosylcobyrinic acid a,c-diamide + L-glutamine + H2O
Pseudomonas denitrificans (nomen rejiciendum)
-
ADP + phosphate + adenosylcobyric acid + L-glutamate
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.16.8.1
6,300-fold purification including Mono Q and FMN agarose chromatography
Pseudomonas denitrificans (nomen rejiciendum)
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.16.8.1
2 cob(II)yrinic acid a,c-diamide + FMN
-
644281
Pseudomonas denitrificans (nomen rejiciendum)
cob(I)yrinic acid a,c-diamide + FMNH2
-
-
-
?
1.16.8.1
cob(II)alamin + FMN
41% activity compared to cob(II)yric acid
644281
Pseudomonas denitrificans (nomen rejiciendum)
?
-
644281
Pseudomonas denitrificans (nomen rejiciendum)
?
1.16.8.1
cob(II)inamide + FMN
67-48% activity compared to cob(II)yric acid, percentage depends on method of activity detection
644281
Pseudomonas denitrificans (nomen rejiciendum)
?
-
644281
Pseudomonas denitrificans (nomen rejiciendum)
?
1.16.8.1
cob(II)inamide phosphate + FMN
66% activity compared to cob(II)yric acid
644281
Pseudomonas denitrificans (nomen rejiciendum)
?
-
644281
Pseudomonas denitrificans (nomen rejiciendum)
?
1.16.8.1
cob(II)yriC acid + FMN
-
644281
Pseudomonas denitrificans (nomen rejiciendum)
?
-
644281
Pseudomonas denitrificans (nomen rejiciendum)
?
1.16.8.1
cob(II)yrinic acid a,c-diamide + FMN
-
644281
Pseudomonas denitrificans (nomen rejiciendum)
cob(I)yrinic acid a,c-diamide + FMNH2
-
644281
Pseudomonas denitrificans (nomen rejiciendum)
?
1.16.8.1
cob(II)yrinic acid a,c-diamide + FMN
52-75% activity compared to cob(II)yric acid, percentage depends on method of activity detection
644281
Pseudomonas denitrificans (nomen rejiciendum)
cob(I)yrinic acid a,c-diamide + FMNH2
-
644281
Pseudomonas denitrificans (nomen rejiciendum)
?
1.16.8.1
cob(II)yrinic acid a,c-diamide + FMN
-
644281
Pseudomonas denitrificans (nomen rejiciendum)
?
-
644281
Pseudomonas denitrificans (nomen rejiciendum)
?
1.16.8.1
GDP-cob(II)inamide + FMN
71% activity compared to cob(II)yric acid
644281
Pseudomonas denitrificans (nomen rejiciendum)
?
-
644281
Pseudomonas denitrificans (nomen rejiciendum)
?
6.3.5.10
ATP + adenosylcobyrinic acid a,c-diamide + L-glutamine + H2O
-
644281
Pseudomonas denitrificans (nomen rejiciendum)
ADP + phosphate + adenosylcobyric acid + L-glutamate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.16.8.1
homodimer
2 * 16000, SDS-PAGE
Pseudomonas denitrificans (nomen rejiciendum)