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Literature summary extracted from

  • Mäki, M.; Järvinen, N.; Räbinä, J.; Roos, C.; Maaheimo, H.; Mattila, P.; Renkonen, R.
    Functional expression of Pseudomonas aeruginosa GDP-4-keto-6-deoxy-D-mannose reductase which synthesizes GDP-rhamnose (2002), Eur. J. Biochem., 269, 593-601.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.281 synthesis generating GDP-rhamnose for in vitro rhamnosylation of glycoproteins and glycopeptides Pseudomonas aeruginosa
1.1.1.281 synthesis the functional expression of the Pseudomonas aeruginosa enzyme in Saccharomyces cerevisiae will provide a tool for generating GDP-rhamnose for in vitro rhamnosylation of glycoprotein and glycopeptides Pseudomonas aeruginosa
1.1.1.281 synthesis generation of GDP-rhamnose by coexpression of enzyme with GDP-mannose-4,6-dehydratase in Saccharomyces cerevisiae Pseudomonas aeruginosa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.281 expression in Saccharomyces cerevisiae Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.281 33900
-
 x * 34000, SDS-PAGE of FLAG-tagged recombinant enzyme, x * 33900, calculated Pseudomonas aeruginosa
1.1.1.281 34000
-
 x * 34000, recombinant FLAG-tagged enzyme Pseudomonas aeruginosa
1.1.1.281 34000
-
 x * 34000, SDS-PAGE of FLAG-tagged recombinant enzyme, x * 33900, calculated Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.281 GDP-4-dehydro-6-deoxy-D-mannose + NADPH Pseudomonas aeruginosa
-
 GDP-6-deoxy-D-mannose + NADP+
-
 ?
1.1.1.281 GDP-6-deoxy-D-mannose + NAD(P)+ Pseudomonas aeruginosa
-
 GDP-4-dehydro-6-deoxy-D-mannose + NAD(P)H
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.281 Pseudomonas aeruginosa
-
-
-
1.1.1.281 Pseudomonas aeruginosa
-
 expression in Saccharomyces cerevisiae
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.281 GDP-4-dehydro-6-deoxy-D-mannose + NADPH
-
 Pseudomonas aeruginosa GDP-6-deoxy-D-mannose + NADP+
-
 ?
1.1.1.281 GDP-6-deoxy-D-lyxo-4-hexulose + NADH + H+
-
 Pseudomonas aeruginosa GDP-alpha-D-rhamnose + NAD+
-
 ir
1.1.1.281 GDP-6-deoxy-D-lyxo-4-hexulose + NADPH + H+
-
 Pseudomonas aeruginosa GDP-alpha-D-rhamnose + NADP+
-
 ir
1.1.1.281 GDP-6-deoxy-D-mannose + NAD(P)+
-
 Pseudomonas aeruginosa GDP-4-dehydro-6-deoxy-D-mannose + NAD(P)H
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.281 ? x * 34000, recombinant FLAG-tagged enzyme Pseudomonas aeruginosa
1.1.1.281 ? x * 34000, SDS-PAGE of FLAG-tagged recombinant enzyme, x * 33900, calculated Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.281 NAD+
-
 Pseudomonas aeruginosa
1.1.1.281 NADH
-
 Pseudomonas aeruginosa
1.1.1.281 NADP+
-
 Pseudomonas aeruginosa
1.1.1.281 NADPH
-
 Pseudomonas aeruginosa